Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury
Our previous study showed that cell cycle exit and neuronal differentiation 1 (CEND1) may participate in neural stem cell cycle exit and oriented differentiation. However, whether CEND1-transfected neural stem cells can improve the prognosis of traumatic brain injury remained unclear. In this study,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=1;spage=130;epage=136;aulast=Wang |
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| author | Ren Wang Dian-Xu Yang Ying-Liang Liu Jun Ding Yan Guo Wan-Hai Ding Heng-Li Tian Fang Yuan |
| author_facet | Ren Wang Dian-Xu Yang Ying-Liang Liu Jun Ding Yan Guo Wan-Hai Ding Heng-Li Tian Fang Yuan |
| author_sort | Ren Wang |
| collection | DOAJ |
| description | Our previous study showed that cell cycle exit and neuronal differentiation 1 (CEND1) may participate in neural stem cell cycle exit and oriented differentiation. However, whether CEND1-transfected neural stem cells can improve the prognosis of traumatic brain injury remained unclear. In this study, we performed quantitative proteomic analysis and found that after traumatic brain injury, CEND1 expression was downregulated in mouse brain tissue. Three days after traumatic brain injury, we transplanted CEND1-transfected neural stem cells into the area surrounding the injury site. We found that at 5 weeks after traumatic brain injury, transplantation of CEND1-transfected neural stem cells markedly alleviated brain atrophy and greatly improved neurological function. In vivo and in vitro results indicate that CEND1 overexpression inhibited the proliferation of neural stem cells, but significantly promoted their neuronal differentiation. Additionally, CEND1 overexpression reduced protein levels of Notch1 and cyclin D1, but increased levels of p21 in CEND1-transfected neural stem cells. Treatment with CEND1-transfected neural stem cells was superior to similar treatment without CEND1 transfection. These findings suggest that transplantation of CEND1-transfected neural stem cells is a promising cell therapy for traumatic brain injury. This study was approved by the Animal Ethics Committee of the School of Biomedical Engineering of Shanghai Jiao Tong University, China (approval No. 2016034) on November 25, 2016. |
| first_indexed | 2024-12-19T06:01:21Z |
| format | Article |
| id | doaj.art-726fa642f4e94f23993f82a4276c7181 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-19T06:01:21Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-726fa642f4e94f23993f82a4276c71812022-12-21T20:33:18ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742022-01-0117113013610.4103/1673-5374.314316Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injuryRen WangDian-Xu YangYing-Liang LiuJun DingYan GuoWan-Hai DingHeng-Li TianFang YuanOur previous study showed that cell cycle exit and neuronal differentiation 1 (CEND1) may participate in neural stem cell cycle exit and oriented differentiation. However, whether CEND1-transfected neural stem cells can improve the prognosis of traumatic brain injury remained unclear. In this study, we performed quantitative proteomic analysis and found that after traumatic brain injury, CEND1 expression was downregulated in mouse brain tissue. Three days after traumatic brain injury, we transplanted CEND1-transfected neural stem cells into the area surrounding the injury site. We found that at 5 weeks after traumatic brain injury, transplantation of CEND1-transfected neural stem cells markedly alleviated brain atrophy and greatly improved neurological function. In vivo and in vitro results indicate that CEND1 overexpression inhibited the proliferation of neural stem cells, but significantly promoted their neuronal differentiation. Additionally, CEND1 overexpression reduced protein levels of Notch1 and cyclin D1, but increased levels of p21 in CEND1-transfected neural stem cells. Treatment with CEND1-transfected neural stem cells was superior to similar treatment without CEND1 transfection. These findings suggest that transplantation of CEND1-transfected neural stem cells is a promising cell therapy for traumatic brain injury. This study was approved by the Animal Ethics Committee of the School of Biomedical Engineering of Shanghai Jiao Tong University, China (approval No. 2016034) on November 25, 2016.http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=1;spage=130;epage=136;aulast=Wangcell cycle exit and neuronal differentiation 1; cyclin d1; embryonic neural stem cells; neuronal differentiation; genetic engineering; overexpression; mice; notch1; p21; traumatic brain injury |
| spellingShingle | Ren Wang Dian-Xu Yang Ying-Liang Liu Jun Ding Yan Guo Wan-Hai Ding Heng-Li Tian Fang Yuan Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury Neural Regeneration Research cell cycle exit and neuronal differentiation 1; cyclin d1; embryonic neural stem cells; neuronal differentiation; genetic engineering; overexpression; mice; notch1; p21; traumatic brain injury |
| title | Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury |
| title_full | Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury |
| title_fullStr | Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury |
| title_full_unstemmed | Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury |
| title_short | Cell cycle exit and neuronal differentiation 1-engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury |
| title_sort | cell cycle exit and neuronal differentiation 1 engineered embryonic neural stem cells enhance neuronal differentiation and neurobehavioral recovery after experimental traumatic brain injury |
| topic | cell cycle exit and neuronal differentiation 1; cyclin d1; embryonic neural stem cells; neuronal differentiation; genetic engineering; overexpression; mice; notch1; p21; traumatic brain injury |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=1;spage=130;epage=136;aulast=Wang |
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