Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.
<h4>Objective</h4>The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates.<h4>Methods</h4>MTBC isolates were identified using standard biochemical procedures, IS6110 PCR,...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21779354/?tool=EBI |
| _version_ | 1819126703892463616 |
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| author | Dorothy Yeboah-Manu Adwoa Asante-Poku Thomas Bodmer David Stucki Kwadwo Koram Frank Bonsu Gerd Pluschke Sebastien Gagneux |
| author_facet | Dorothy Yeboah-Manu Adwoa Asante-Poku Thomas Bodmer David Stucki Kwadwo Koram Frank Bonsu Gerd Pluschke Sebastien Gagneux |
| author_sort | Dorothy Yeboah-Manu |
| collection | DOAJ |
| description | <h4>Objective</h4>The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates.<h4>Methods</h4>MTBC isolates were identified using standard biochemical procedures, IS6110 PCR, and large sequence polymorphisms. Isolates were further typed using spoligotyping, and the phenotypic drug susceptibility patterns were determined by the proportion method.<h4>Result</h4>One hundred and sixty-two isolates were characterised by LSP typing. Of these, 130 (80.25%) were identified as Mycobacterium tuberculosis sensu stricto (MTBss), with the Cameroon sub-lineage being dominant (N = 59/130, 45.38%). Thirty-two (19.75%) isolates were classified as Mycobacterium africanum type 1, and of these 26 (81.25%) were identified as West-Africa I, and 6 (18.75%) as West-Africa II. Spoligotyping sub-lineages identified among the MTBss included Haarlem (N = 15, 11.53%), Ghana (N = 22, 16.92%), Beijing (4, 3.08%), EAI (4, 3.08%), Uganda I (4, 3.08%), LAM (2, 1.54%), X (N = 1, 0.77%) and S (2, 1.54%). Nine isolates had SIT numbers with no identified sub-lineages while 17 had no SIT numbers. MTBss isolates were more likely to be resistant to streptomycin (p<0.008) and to any drug resistance (p<0.03) when compared to M. africanum.<h4>Conclusion</h4>This study demonstrated that overall 36.4% of TB in South-Western Ghana is caused by the Cameroon sub-lineage of MTBC and 20% by M. africanum type 1, including both the West-Africa 1 and West-Africa 2 lineages. The diversity of MTBC in Ghana should be considered when evaluating new TB vaccines. |
| first_indexed | 2024-12-22T08:00:15Z |
| format | Article |
| id | doaj.art-727324ebd1e44f6784e3cbfa84567a9c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T08:00:15Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-727324ebd1e44f6784e3cbfa84567a9c2022-12-21T18:33:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0167e2190610.1371/journal.pone.0021906Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana.Dorothy Yeboah-ManuAdwoa Asante-PokuThomas BodmerDavid StuckiKwadwo KoramFrank BonsuGerd PluschkeSebastien Gagneux<h4>Objective</h4>The aim of this study was to use spoligotyping and large sequence polymorphism (LSP) to study the population structure of M. tuberculosis complex (MTBC) isolates.<h4>Methods</h4>MTBC isolates were identified using standard biochemical procedures, IS6110 PCR, and large sequence polymorphisms. Isolates were further typed using spoligotyping, and the phenotypic drug susceptibility patterns were determined by the proportion method.<h4>Result</h4>One hundred and sixty-two isolates were characterised by LSP typing. Of these, 130 (80.25%) were identified as Mycobacterium tuberculosis sensu stricto (MTBss), with the Cameroon sub-lineage being dominant (N = 59/130, 45.38%). Thirty-two (19.75%) isolates were classified as Mycobacterium africanum type 1, and of these 26 (81.25%) were identified as West-Africa I, and 6 (18.75%) as West-Africa II. Spoligotyping sub-lineages identified among the MTBss included Haarlem (N = 15, 11.53%), Ghana (N = 22, 16.92%), Beijing (4, 3.08%), EAI (4, 3.08%), Uganda I (4, 3.08%), LAM (2, 1.54%), X (N = 1, 0.77%) and S (2, 1.54%). Nine isolates had SIT numbers with no identified sub-lineages while 17 had no SIT numbers. MTBss isolates were more likely to be resistant to streptomycin (p<0.008) and to any drug resistance (p<0.03) when compared to M. africanum.<h4>Conclusion</h4>This study demonstrated that overall 36.4% of TB in South-Western Ghana is caused by the Cameroon sub-lineage of MTBC and 20% by M. africanum type 1, including both the West-Africa 1 and West-Africa 2 lineages. The diversity of MTBC in Ghana should be considered when evaluating new TB vaccines.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21779354/?tool=EBI |
| spellingShingle | Dorothy Yeboah-Manu Adwoa Asante-Poku Thomas Bodmer David Stucki Kwadwo Koram Frank Bonsu Gerd Pluschke Sebastien Gagneux Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana. PLoS ONE |
| title | Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana. |
| title_full | Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana. |
| title_fullStr | Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana. |
| title_full_unstemmed | Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana. |
| title_short | Genotypic diversity and drug susceptibility patterns among M. tuberculosis complex isolates from South-Western Ghana. |
| title_sort | genotypic diversity and drug susceptibility patterns among m tuberculosis complex isolates from south western ghana |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21779354/?tool=EBI |
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