<i>Streptomycetaceae</i> and <i>Promicromonosporaceae</i>: Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural Phosphate
Soil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four different rhizospheric Moroccan oat soils and to inves...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/6/1116 |
| _version_ | 1797484269436665856 |
|---|---|
| author | Meriam Bousselham Sanaa Lemriss Driss Dhiba Yassine Aallam Amal Souiri Younes Abbas Nezha Saïdi Hassan Boukcim Hanane Hamdali |
| author_facet | Meriam Bousselham Sanaa Lemriss Driss Dhiba Yassine Aallam Amal Souiri Younes Abbas Nezha Saïdi Hassan Boukcim Hanane Hamdali |
| author_sort | Meriam Bousselham |
| collection | DOAJ |
| description | Soil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four different rhizospheric Moroccan oat soils and to investigate their potential for P solubilization. The distribution of actinomycetes was significantly more abundant in Settat (9.68%), Tangier (7.38%), and Beni Mellal (6.87%) than in the Merchouch-Rabat (4.90%) region. A total of 235 actinomycete strains were isolated from all sites and tested for their ability to grow on a synthetic minimum medium (SMM) containing insoluble natural rock phosphate (RP) or synthetic tricalcium phosphate (TCP) as the unique P source. One hundred forty-three isolates (60.8%) had the ability to grow in the SMM with RP whereas only twenty-five isolates (17%) had the most active growth using the SMM with TCP. Eight isolates with the most active growth in solid SMM were selected for their P solubilization abilities in liquid SMM cultures. The highest amount of P solubilized was 163.8 µg/mL for RP and 110.27 µg/mL for TCP after 5 days of culture. The biosolubilization process of AM2, the most efficient RP and TCP solubilizing strain, probably implied the highest excretion of siderophore substances. Eight of these strains were shown to belong to the <i>Streptomyces</i> genus and one to the <i>Promicromonospora</i> genus. These findings bolster the phosphate biosolubilization abilities of actinomycetes and may participate in increasing agricultural yields in an eco-efficient and environmentally friendly manner. |
| first_indexed | 2024-03-09T23:00:05Z |
| format | Article |
| id | doaj.art-72739030505c4a6da7bd1bd309278172 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:00:05Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-72739030505c4a6da7bd1bd3092781722023-11-23T18:03:15ZengMDPI AGMicroorganisms2076-26072022-05-01106111610.3390/microorganisms10061116<i>Streptomycetaceae</i> and <i>Promicromonosporaceae</i>: Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural PhosphateMeriam Bousselham0Sanaa Lemriss1Driss Dhiba2Yassine Aallam3Amal Souiri4Younes Abbas5Nezha Saïdi6Hassan Boukcim7Hanane Hamdali8Laboratory of Agro-Industrial and Medical Biotechnologies, Faculty of Sciences and Technology, University of Sultan Moulay Slimane, Mghila BP 523, Beni-Mellal 23000, MoroccoLaboratory of Agro-Industrial and Medical Biotechnologies, Faculty of Sciences and Technology, University of Sultan Moulay Slimane, Mghila BP 523, Beni-Mellal 23000, MoroccoInternational Water Research Institute, University Mohammed 6 Polytechnic (UM6P), Hay Moulay Rachid, Ben Guerir 43150, MoroccoLaboratory of Agro-Industrial and Medical Biotechnologies, Faculty of Sciences and Technology, University of Sultan Moulay Slimane, Mghila BP 523, Beni-Mellal 23000, MoroccoLaboratory of Agro-Industrial and Medical Biotechnologies, Faculty of Sciences and Technology, University of Sultan Moulay Slimane, Mghila BP 523, Beni-Mellal 23000, MoroccoPolydisciplinary Faculty of Beni Mellal, University of Sultan Moulay Slimane, Mghila BP 523, Beni-Mellal 23000, MoroccoCRRA Rabat, Plant Breeding, Conservation and Valorization of Plant Genetic Resources Research Unit, Rabat Institutes, P.O. Box 6570, Rabat 10101, MoroccoScientific Parc Agropolis, 1900 la Lironde, PSIII Boulevard, F34980 Montferrier sur Lez, FranceLaboratory of Agro-Industrial and Medical Biotechnologies, Faculty of Sciences and Technology, University of Sultan Moulay Slimane, Mghila BP 523, Beni-Mellal 23000, MoroccoSoil actinomycetes explorations appear to be an efficient alternative as biofertilizers to optimize the use of phosphorus (P) resources and enhance plant growth. This research aimed to explore the distribution of actinomycetes isolated from four different rhizospheric Moroccan oat soils and to investigate their potential for P solubilization. The distribution of actinomycetes was significantly more abundant in Settat (9.68%), Tangier (7.38%), and Beni Mellal (6.87%) than in the Merchouch-Rabat (4.90%) region. A total of 235 actinomycete strains were isolated from all sites and tested for their ability to grow on a synthetic minimum medium (SMM) containing insoluble natural rock phosphate (RP) or synthetic tricalcium phosphate (TCP) as the unique P source. One hundred forty-three isolates (60.8%) had the ability to grow in the SMM with RP whereas only twenty-five isolates (17%) had the most active growth using the SMM with TCP. Eight isolates with the most active growth in solid SMM were selected for their P solubilization abilities in liquid SMM cultures. The highest amount of P solubilized was 163.8 µg/mL for RP and 110.27 µg/mL for TCP after 5 days of culture. The biosolubilization process of AM2, the most efficient RP and TCP solubilizing strain, probably implied the highest excretion of siderophore substances. Eight of these strains were shown to belong to the <i>Streptomyces</i> genus and one to the <i>Promicromonospora</i> genus. These findings bolster the phosphate biosolubilization abilities of actinomycetes and may participate in increasing agricultural yields in an eco-efficient and environmentally friendly manner.https://www.mdpi.com/2076-2607/10/6/1116<i>Streptomyces</i><i>Promicromonospora</i>biosolubilizationbiofertilizersrock phosphateMoroccan oat soils |
| spellingShingle | Meriam Bousselham Sanaa Lemriss Driss Dhiba Yassine Aallam Amal Souiri Younes Abbas Nezha Saïdi Hassan Boukcim Hanane Hamdali <i>Streptomycetaceae</i> and <i>Promicromonosporaceae</i>: Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural Phosphate Microorganisms <i>Streptomyces</i> <i>Promicromonospora</i> biosolubilization biofertilizers rock phosphate Moroccan oat soils |
| title | <i>Streptomycetaceae</i> and <i>Promicromonosporaceae</i>: Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural Phosphate |
| title_full | <i>Streptomycetaceae</i> and <i>Promicromonosporaceae</i>: Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural Phosphate |
| title_fullStr | <i>Streptomycetaceae</i> and <i>Promicromonosporaceae</i>: Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural Phosphate |
| title_full_unstemmed | <i>Streptomycetaceae</i> and <i>Promicromonosporaceae</i>: Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural Phosphate |
| title_short | <i>Streptomycetaceae</i> and <i>Promicromonosporaceae</i>: Two Actinomycetes Families from Moroccan Oat Soils Enhancing Solubilization of Natural Phosphate |
| title_sort | i streptomycetaceae i and i promicromonosporaceae i two actinomycetes families from moroccan oat soils enhancing solubilization of natural phosphate |
| topic | <i>Streptomyces</i> <i>Promicromonospora</i> biosolubilization biofertilizers rock phosphate Moroccan oat soils |
| url | https://www.mdpi.com/2076-2607/10/6/1116 |
| work_keys_str_mv | AT meriambousselham istreptomycetaceaeiandipromicromonosporaceaeitwoactinomycetesfamiliesfrommoroccanoatsoilsenhancingsolubilizationofnaturalphosphate AT sanaalemriss istreptomycetaceaeiandipromicromonosporaceaeitwoactinomycetesfamiliesfrommoroccanoatsoilsenhancingsolubilizationofnaturalphosphate AT drissdhiba istreptomycetaceaeiandipromicromonosporaceaeitwoactinomycetesfamiliesfrommoroccanoatsoilsenhancingsolubilizationofnaturalphosphate AT yassineaallam istreptomycetaceaeiandipromicromonosporaceaeitwoactinomycetesfamiliesfrommoroccanoatsoilsenhancingsolubilizationofnaturalphosphate AT amalsouiri istreptomycetaceaeiandipromicromonosporaceaeitwoactinomycetesfamiliesfrommoroccanoatsoilsenhancingsolubilizationofnaturalphosphate AT younesabbas istreptomycetaceaeiandipromicromonosporaceaeitwoactinomycetesfamiliesfrommoroccanoatsoilsenhancingsolubilizationofnaturalphosphate AT nezhasaidi istreptomycetaceaeiandipromicromonosporaceaeitwoactinomycetesfamiliesfrommoroccanoatsoilsenhancingsolubilizationofnaturalphosphate AT hassanboukcim istreptomycetaceaeiandipromicromonosporaceaeitwoactinomycetesfamiliesfrommoroccanoatsoilsenhancingsolubilizationofnaturalphosphate AT hananehamdali istreptomycetaceaeiandipromicromonosporaceaeitwoactinomycetesfamiliesfrommoroccanoatsoilsenhancingsolubilizationofnaturalphosphate |