Quality Assessment of Mycobacterium tuberculosis Genotyping in a Large Laboratory Network
Quality assessment exercises were conducted to evaluate the reproducibility of IS6110 DNA fingerprinting performed by eight laboratories in the National Tuberculosis Genotyping and Surveillance Network. Three panels, each with 8 to 16 isolates, were typed at all laboratories, resulting in 280 images...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2002-11-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/8/11/02-0401_article |
| _version_ | 1818176289497939968 |
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| author | Christopher R. Braden Jack T. Crawford Barbara A. Schable |
| author_facet | Christopher R. Braden Jack T. Crawford Barbara A. Schable |
| author_sort | Christopher R. Braden |
| collection | DOAJ |
| description | Quality assessment exercises were conducted to evaluate the reproducibility of IS6110 DNA fingerprinting performed by eight laboratories in the National Tuberculosis Genotyping and Surveillance Network. Three panels, each with 8 to 16 isolates, were typed at all laboratories, resulting in 280 images. When the pattern obtained by the majority for each isolate was used as the standard, exact matches were obtained for 73% of patterns; 90% and 97% of patterns matched within one- and two-band differences, respectively. A second approach involved retyping of randomly selected isolates at the Centers for Disease Control and Prevention. Retyping was done for 8–19 isolates per laboratory (76 total). Paired images matched exactly for 54% of isolates and within one and two band differences, 78% and 93%, respectively. We evaluated reasons for mismatching. We also evaluated the reproducibility of spoligotyping using a test panel of 13 isolates; a discrepancy of 1 in 91 results was noted. |
| first_indexed | 2024-12-11T20:13:49Z |
| format | Article |
| id | doaj.art-7274a9e683114873b586c16b3cc7b057 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-11T20:13:49Z |
| publishDate | 2002-11-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-7274a9e683114873b586c16b3cc7b0572022-12-22T00:52:14ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592002-11-018111210121510.3201/eid0811.020401Quality Assessment of Mycobacterium tuberculosis Genotyping in a Large Laboratory NetworkChristopher R. BradenJack T. CrawfordBarbara A. SchableQuality assessment exercises were conducted to evaluate the reproducibility of IS6110 DNA fingerprinting performed by eight laboratories in the National Tuberculosis Genotyping and Surveillance Network. Three panels, each with 8 to 16 isolates, were typed at all laboratories, resulting in 280 images. When the pattern obtained by the majority for each isolate was used as the standard, exact matches were obtained for 73% of patterns; 90% and 97% of patterns matched within one- and two-band differences, respectively. A second approach involved retyping of randomly selected isolates at the Centers for Disease Control and Prevention. Retyping was done for 8–19 isolates per laboratory (76 total). Paired images matched exactly for 54% of isolates and within one and two band differences, 78% and 93%, respectively. We evaluated reasons for mismatching. We also evaluated the reproducibility of spoligotyping using a test panel of 13 isolates; a discrepancy of 1 in 91 results was noted.https://wwwnc.cdc.gov/eid/article/8/11/02-0401_articleMycobacterium tuberculosisDNA fingerprintingquality assessmentmolecular epidemiologyUnited States |
| spellingShingle | Christopher R. Braden Jack T. Crawford Barbara A. Schable Quality Assessment of Mycobacterium tuberculosis Genotyping in a Large Laboratory Network Emerging Infectious Diseases Mycobacterium tuberculosis DNA fingerprinting quality assessment molecular epidemiology United States |
| title | Quality Assessment of Mycobacterium tuberculosis Genotyping in a Large Laboratory Network |
| title_full | Quality Assessment of Mycobacterium tuberculosis Genotyping in a Large Laboratory Network |
| title_fullStr | Quality Assessment of Mycobacterium tuberculosis Genotyping in a Large Laboratory Network |
| title_full_unstemmed | Quality Assessment of Mycobacterium tuberculosis Genotyping in a Large Laboratory Network |
| title_short | Quality Assessment of Mycobacterium tuberculosis Genotyping in a Large Laboratory Network |
| title_sort | quality assessment of mycobacterium tuberculosis genotyping in a large laboratory network |
| topic | Mycobacterium tuberculosis DNA fingerprinting quality assessment molecular epidemiology United States |
| url | https://wwwnc.cdc.gov/eid/article/8/11/02-0401_article |
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