Lateral Root Development in Potato Is Mediated by Stu-mi164 Regulation of NAC Transcription Factor
The NAC designation is derived from petunia (Petunia hybrida) gene NO APICAL MERISTEM (NAM) and Arabidopsis genes ATAF1/ATAF2 and CUP-SHAPED COTYLEDON2 (CUC2), which belongs to the family of plant-specific transcription factors (TFs), and plays important role in plant development processes, such as...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00383/full |
| _version_ | 1828806886910066688 |
|---|---|
| author | Li Zhang Li Zhang Lei Yao Lei Yao Ning Zhang Ning Zhang Jiangwei Yang Jiangwei Yang Xi Zhu Xi Zhu Xun Tang Xun Tang Alejandro Calderón-Urrea Alejandro Calderón-Urrea Huaijun Si Huaijun Si |
| author_facet | Li Zhang Li Zhang Lei Yao Lei Yao Ning Zhang Ning Zhang Jiangwei Yang Jiangwei Yang Xi Zhu Xi Zhu Xun Tang Xun Tang Alejandro Calderón-Urrea Alejandro Calderón-Urrea Huaijun Si Huaijun Si |
| author_sort | Li Zhang |
| collection | DOAJ |
| description | The NAC designation is derived from petunia (Petunia hybrida) gene NO APICAL MERISTEM (NAM) and Arabidopsis genes ATAF1/ATAF2 and CUP-SHAPED COTYLEDON2 (CUC2), which belongs to the family of plant-specific transcription factors (TFs), and plays important role in plant development processes, such as response to biotic and abiotic stress, and hormone signaling. MicroRNAs (miRNAs) are a class of small, non-coding endogenous RNAs which play versatile and significant role in plant stress response and development via negatively affecting gene expression at a post-transcriptional level. Here, we showed that Stu-mi164 had a complementary sequence in the CDS sequence of potato NAC TFs, and that NAC expression exhibited significant differences under osmotic stress. We measured expression levels of the Stu-mi164 target gene StNAC262 between control and PEG-treated plants using real-time PCR, and the results demonstrated that they had inverse relationship. We suggested that Stu-miR164 might drive overexpression of NAC gene under osmotic stress in potato. To confirm the regulation of NAC TFs by Stu-mi164, we developed transgenic plants, using Agrobacterium tumefaciens–mediated transformation, of the potato cultivars “Gannongshu 2” and “Kexin 3” overexpressing the Stu-mi164 or the TF StNAC262. Real-time PCR analysis of transgenic potato plants under osmotic (PEG) stress, showed that potato plants overexpressing Stu-mi164 had reduced expression of StNAC262 and their osmotic resistance decreased. Furthermore, these plants had low number of lateral roots although the same length as the control. Our findings support the regulatory role of Stu-miRNAs in controlling plant response to osmotic stress via StNAC262. |
| first_indexed | 2024-12-12T08:17:10Z |
| format | Article |
| id | doaj.art-72777fac3f6e44baba26a53b13a90d12 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T08:17:10Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-72777fac3f6e44baba26a53b13a90d122022-12-22T00:31:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-03-01910.3389/fpls.2018.00383336941Lateral Root Development in Potato Is Mediated by Stu-mi164 Regulation of NAC Transcription FactorLi Zhang0Li Zhang1Lei Yao2Lei Yao3Ning Zhang4Ning Zhang5Jiangwei Yang6Jiangwei Yang7Xi Zhu8Xi Zhu9Xun Tang10Xun Tang11Alejandro Calderón-Urrea12Alejandro Calderón-Urrea13Huaijun Si14Huaijun Si15Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaCollege of Plant Protection, Gansu Agricultural University, Lanzhou, ChinaDepartment of Biology, California State University, Fresno, CA, United StatesGansu Provincial Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement, Gansu Agricultural University, Lanzhou, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou, ChinaThe NAC designation is derived from petunia (Petunia hybrida) gene NO APICAL MERISTEM (NAM) and Arabidopsis genes ATAF1/ATAF2 and CUP-SHAPED COTYLEDON2 (CUC2), which belongs to the family of plant-specific transcription factors (TFs), and plays important role in plant development processes, such as response to biotic and abiotic stress, and hormone signaling. MicroRNAs (miRNAs) are a class of small, non-coding endogenous RNAs which play versatile and significant role in plant stress response and development via negatively affecting gene expression at a post-transcriptional level. Here, we showed that Stu-mi164 had a complementary sequence in the CDS sequence of potato NAC TFs, and that NAC expression exhibited significant differences under osmotic stress. We measured expression levels of the Stu-mi164 target gene StNAC262 between control and PEG-treated plants using real-time PCR, and the results demonstrated that they had inverse relationship. We suggested that Stu-miR164 might drive overexpression of NAC gene under osmotic stress in potato. To confirm the regulation of NAC TFs by Stu-mi164, we developed transgenic plants, using Agrobacterium tumefaciens–mediated transformation, of the potato cultivars “Gannongshu 2” and “Kexin 3” overexpressing the Stu-mi164 or the TF StNAC262. Real-time PCR analysis of transgenic potato plants under osmotic (PEG) stress, showed that potato plants overexpressing Stu-mi164 had reduced expression of StNAC262 and their osmotic resistance decreased. Furthermore, these plants had low number of lateral roots although the same length as the control. Our findings support the regulatory role of Stu-miRNAs in controlling plant response to osmotic stress via StNAC262.http://journal.frontiersin.org/article/10.3389/fpls.2018.00383/fullmiRNANACosmotic stresslateral rootpotato |
| spellingShingle | Li Zhang Li Zhang Lei Yao Lei Yao Ning Zhang Ning Zhang Jiangwei Yang Jiangwei Yang Xi Zhu Xi Zhu Xun Tang Xun Tang Alejandro Calderón-Urrea Alejandro Calderón-Urrea Huaijun Si Huaijun Si Lateral Root Development in Potato Is Mediated by Stu-mi164 Regulation of NAC Transcription Factor Frontiers in Plant Science miRNA NAC osmotic stress lateral root potato |
| title | Lateral Root Development in Potato Is Mediated by Stu-mi164 Regulation of NAC Transcription Factor |
| title_full | Lateral Root Development in Potato Is Mediated by Stu-mi164 Regulation of NAC Transcription Factor |
| title_fullStr | Lateral Root Development in Potato Is Mediated by Stu-mi164 Regulation of NAC Transcription Factor |
| title_full_unstemmed | Lateral Root Development in Potato Is Mediated by Stu-mi164 Regulation of NAC Transcription Factor |
| title_short | Lateral Root Development in Potato Is Mediated by Stu-mi164 Regulation of NAC Transcription Factor |
| title_sort | lateral root development in potato is mediated by stu mi164 regulation of nac transcription factor |
| topic | miRNA NAC osmotic stress lateral root potato |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00383/full |
| work_keys_str_mv | AT lizhang lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT lizhang lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT leiyao lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT leiyao lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT ningzhang lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT ningzhang lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT jiangweiyang lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT jiangweiyang lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT xizhu lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT xizhu lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT xuntang lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT xuntang lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT alejandrocalderonurrea lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT alejandrocalderonurrea lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT huaijunsi lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor AT huaijunsi lateralrootdevelopmentinpotatoismediatedbystumi164regulationofnactranscriptionfactor |