Transcriptome Profiling of m<sup>6</sup>A mRNA Modification in Bovine Mammary Epithelial Cells Treated with <i>Escherichia coli</i>
Mastitis is a common disease in dairy cows that is mostly caused by <i>E. coli</i>, and it brings massive losses to the dairy industry. <i>N</i><sup>6</sup>-Methyladenosine (m<sup>6</sup>A), a methylation at the <i>N</i><sup>6</sup...
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MDPI AG
2021-06-01
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| Online Access: | https://www.mdpi.com/1422-0067/22/12/6254 |
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| author | Ting Li Changjie Lin Yifan Zhu Haojun Xu Yiya Yin Chaohao Wang Xin Tang Tongxing Song Aizhen Guo Yingyu Chen Changmin Hu |
| author_facet | Ting Li Changjie Lin Yifan Zhu Haojun Xu Yiya Yin Chaohao Wang Xin Tang Tongxing Song Aizhen Guo Yingyu Chen Changmin Hu |
| author_sort | Ting Li |
| collection | DOAJ |
| description | Mastitis is a common disease in dairy cows that is mostly caused by <i>E. coli</i>, and it brings massive losses to the dairy industry. <i>N</i><sup>6</sup>-Methyladenosine (m<sup>6</sup>A), a methylation at the <i>N</i><sup>6</sup> position of RNA adenine, is a type of modification strongly associated with many diseases. However, the role of m<sup>6</sup>A in mastitis has not been investigated. In this study, we used MeRIP-seq to sequence the RNA of bovine mammary epithelial cells treated with inactivated <i>E. coli</i> for 24 h. In this in vitro infection model, there were 16,691 m<sup>6</sup>A peaks within 7066 mRNA transcripts in the Con group and 10,029 peaks within 4891 transcripts in the <i>E. coli</i> group. Compared with the Con group, 474 mRNAs were hypermethylated and 2101 mRNAs were hypomethylated in the <i>E. coli</i> group. Biological function analyses revealed differential m<sup>6</sup>A-modified genes mainly enriched in the MAPK, NF-κB, and TGF-β signaling pathways. In order to explore the relationship between m<sup>6</sup>A and mRNA expression, combined MeRIP-seq and mRNA-seq analyses revealed 212 genes with concomitant changes in the mRNA expression and m<sup>6</sup>A modification. This study is the first to present a map of RNA m<sup>6</sup>A modification in mastitis treated with <i>E. coli</i>, providing a basis for future research. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:32:17Z |
| publishDate | 2021-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-72791ba761e248d0b9ea2de3751680f82023-11-21T23:34:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212625410.3390/ijms22126254Transcriptome Profiling of m<sup>6</sup>A mRNA Modification in Bovine Mammary Epithelial Cells Treated with <i>Escherichia coli</i>Ting Li0Changjie Lin1Yifan Zhu2Haojun Xu3Yiya Yin4Chaohao Wang5Xin Tang6Tongxing Song7Aizhen Guo8Yingyu Chen9Changmin Hu10Department of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Animal Nutrition and Feed Science College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Clinical Veterinary Medicine, Faculty of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, ChinaMastitis is a common disease in dairy cows that is mostly caused by <i>E. coli</i>, and it brings massive losses to the dairy industry. <i>N</i><sup>6</sup>-Methyladenosine (m<sup>6</sup>A), a methylation at the <i>N</i><sup>6</sup> position of RNA adenine, is a type of modification strongly associated with many diseases. However, the role of m<sup>6</sup>A in mastitis has not been investigated. In this study, we used MeRIP-seq to sequence the RNA of bovine mammary epithelial cells treated with inactivated <i>E. coli</i> for 24 h. In this in vitro infection model, there were 16,691 m<sup>6</sup>A peaks within 7066 mRNA transcripts in the Con group and 10,029 peaks within 4891 transcripts in the <i>E. coli</i> group. Compared with the Con group, 474 mRNAs were hypermethylated and 2101 mRNAs were hypomethylated in the <i>E. coli</i> group. Biological function analyses revealed differential m<sup>6</sup>A-modified genes mainly enriched in the MAPK, NF-κB, and TGF-β signaling pathways. In order to explore the relationship between m<sup>6</sup>A and mRNA expression, combined MeRIP-seq and mRNA-seq analyses revealed 212 genes with concomitant changes in the mRNA expression and m<sup>6</sup>A modification. This study is the first to present a map of RNA m<sup>6</sup>A modification in mastitis treated with <i>E. coli</i>, providing a basis for future research.https://www.mdpi.com/1422-0067/22/12/6254mastitisMeRIP-seq<i>E. coli</i>m<sup>6</sup>A modification<i>N</i><sup>6</sup>-methyladenosine |
| spellingShingle | Ting Li Changjie Lin Yifan Zhu Haojun Xu Yiya Yin Chaohao Wang Xin Tang Tongxing Song Aizhen Guo Yingyu Chen Changmin Hu Transcriptome Profiling of m<sup>6</sup>A mRNA Modification in Bovine Mammary Epithelial Cells Treated with <i>Escherichia coli</i> International Journal of Molecular Sciences mastitis MeRIP-seq <i>E. coli</i> m<sup>6</sup>A modification <i>N</i><sup>6</sup>-methyladenosine |
| title | Transcriptome Profiling of m<sup>6</sup>A mRNA Modification in Bovine Mammary Epithelial Cells Treated with <i>Escherichia coli</i> |
| title_full | Transcriptome Profiling of m<sup>6</sup>A mRNA Modification in Bovine Mammary Epithelial Cells Treated with <i>Escherichia coli</i> |
| title_fullStr | Transcriptome Profiling of m<sup>6</sup>A mRNA Modification in Bovine Mammary Epithelial Cells Treated with <i>Escherichia coli</i> |
| title_full_unstemmed | Transcriptome Profiling of m<sup>6</sup>A mRNA Modification in Bovine Mammary Epithelial Cells Treated with <i>Escherichia coli</i> |
| title_short | Transcriptome Profiling of m<sup>6</sup>A mRNA Modification in Bovine Mammary Epithelial Cells Treated with <i>Escherichia coli</i> |
| title_sort | transcriptome profiling of m sup 6 sup a mrna modification in bovine mammary epithelial cells treated with i escherichia coli i |
| topic | mastitis MeRIP-seq <i>E. coli</i> m<sup>6</sup>A modification <i>N</i><sup>6</sup>-methyladenosine |
| url | https://www.mdpi.com/1422-0067/22/12/6254 |
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