Sperm Motility of Mice under Simulated Microgravity and Hypergravity
For deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse...
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/14/5054 |
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| author | Irina V. Ogneva Maria A. Usik Nikolay S. Biryukov Yuliya S. Zhdankina |
| author_facet | Irina V. Ogneva Maria A. Usik Nikolay S. Biryukov Yuliya S. Zhdankina |
| author_sort | Irina V. Ogneva |
| collection | DOAJ |
| description | For deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse sperm motility and the mechanisms of this change. For 1, 3 and 6 h, mouse sperm samples isolated from the caudal epididymis were subjected to simulated microgravity using a random position machine and 2g hypergravity using a centrifuge. The experimental samples were compared with static and dynamic controls. The sperm motility and the percentage of motile sperm were determined using microscopy and video analysis, cell respiration was determined by polarography, the protein content was assessed by Western blotting and the mRNA levels were determined using qRT-PCR. The results indicated that hypergravity conditions led to more significant changes than simulated microgravity conditions: after 1 h, the speed of sperm movement decreased, and after 3 h, the number of motile cells began to decrease. Under the microgravity model, the speed of movement did not change, but the motile spermatozoa decreased after 6 h of exposure. These changes are likely associated with a change in the structure of the microtubule cytoskeleton, and changes in the energy supply are an adaptive reaction to changes in sperm motility. |
| first_indexed | 2024-03-10T18:24:44Z |
| format | Article |
| id | doaj.art-72792e48fa7444b1b1a18683479ded9c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:24:44Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-72792e48fa7444b1b1a18683479ded9c2023-11-20T07:06:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012114505410.3390/ijms21145054Sperm Motility of Mice under Simulated Microgravity and HypergravityIrina V. Ogneva0Maria A. Usik1Nikolay S. Biryukov2Yuliya S. Zhdankina3Cell Biophysics Laboratory, State Scientific Center of the Russian Federation, Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe Shosse, Moscow 123007, RussiaCell Biophysics Laboratory, State Scientific Center of the Russian Federation, Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe Shosse, Moscow 123007, RussiaCell Biophysics Laboratory, State Scientific Center of the Russian Federation, Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe Shosse, Moscow 123007, RussiaCell Biophysics Laboratory, State Scientific Center of the Russian Federation, Institute of Biomedical Problems of the Russian Academy of Sciences, 76a, Khoroshevskoyoe Shosse, Moscow 123007, RussiaFor deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse sperm motility and the mechanisms of this change. For 1, 3 and 6 h, mouse sperm samples isolated from the caudal epididymis were subjected to simulated microgravity using a random position machine and 2g hypergravity using a centrifuge. The experimental samples were compared with static and dynamic controls. The sperm motility and the percentage of motile sperm were determined using microscopy and video analysis, cell respiration was determined by polarography, the protein content was assessed by Western blotting and the mRNA levels were determined using qRT-PCR. The results indicated that hypergravity conditions led to more significant changes than simulated microgravity conditions: after 1 h, the speed of sperm movement decreased, and after 3 h, the number of motile cells began to decrease. Under the microgravity model, the speed of movement did not change, but the motile spermatozoa decreased after 6 h of exposure. These changes are likely associated with a change in the structure of the microtubule cytoskeleton, and changes in the energy supply are an adaptive reaction to changes in sperm motility.https://www.mdpi.com/1422-0067/21/14/5054sperm motilitycell respirationcytoskeletonsimulated microgravityhypergravity |
| spellingShingle | Irina V. Ogneva Maria A. Usik Nikolay S. Biryukov Yuliya S. Zhdankina Sperm Motility of Mice under Simulated Microgravity and Hypergravity International Journal of Molecular Sciences sperm motility cell respiration cytoskeleton simulated microgravity hypergravity |
| title | Sperm Motility of Mice under Simulated Microgravity and Hypergravity |
| title_full | Sperm Motility of Mice under Simulated Microgravity and Hypergravity |
| title_fullStr | Sperm Motility of Mice under Simulated Microgravity and Hypergravity |
| title_full_unstemmed | Sperm Motility of Mice under Simulated Microgravity and Hypergravity |
| title_short | Sperm Motility of Mice under Simulated Microgravity and Hypergravity |
| title_sort | sperm motility of mice under simulated microgravity and hypergravity |
| topic | sperm motility cell respiration cytoskeleton simulated microgravity hypergravity |
| url | https://www.mdpi.com/1422-0067/21/14/5054 |
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