Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae.
Upon glucose starvation, S. cerevisiae shows a dramatic alteration in transcription, resulting in wide-scale repression of most genes and activation of some others. This coincides with an arrest of cellular proliferation. A subset of such cells enters quiescence, a reversible non-dividing state. Her...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-12-01
|
| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1010559 |
| _version_ | 1811167241241100288 |
|---|---|
| author | Thomas B Bailey Phaedra A Whitty Eric U Selker Jeffrey N McKnight Laura E McKnight |
| author_facet | Thomas B Bailey Phaedra A Whitty Eric U Selker Jeffrey N McKnight Laura E McKnight |
| author_sort | Thomas B Bailey |
| collection | DOAJ |
| description | Upon glucose starvation, S. cerevisiae shows a dramatic alteration in transcription, resulting in wide-scale repression of most genes and activation of some others. This coincides with an arrest of cellular proliferation. A subset of such cells enters quiescence, a reversible non-dividing state. Here, we demonstrate that the conserved transcriptional corepressor Tup1 is critical for transcriptional repression after glucose depletion. We show that Tup1-Ssn6 binds new targets upon glucose depletion, where it remains as the cells enter the G0 phase of the cell cycle. In addition, we show that Tup1 represses a variety of glucose metabolism and transport genes. We explored how Tup1 mediated repression is accomplished and demonstrated that Tup1 coordinates with the Rpd3L complex to deacetylate H3K23. We found that Tup1 coordinates with Isw2 to affect nucleosome positions at glucose transporter HXT family genes during G0. Finally, microscopy revealed that a quarter of cells with a Tup1 deletion contain multiple DAPI puncta. Taken together, these findings demonstrate the role of Tup1 in transcriptional reprogramming in response to environmental cues leading to the quiescent state. |
| first_indexed | 2024-04-10T16:05:58Z |
| format | Article |
| id | doaj.art-72794eba61354e779721f234b88cc24d |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-10T16:05:58Z |
| publishDate | 2022-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-72794eba61354e779721f234b88cc24d2023-02-10T05:31:18ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042022-12-011812e101055910.1371/journal.pgen.1010559Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae.Thomas B BaileyPhaedra A WhittyEric U SelkerJeffrey N McKnightLaura E McKnightUpon glucose starvation, S. cerevisiae shows a dramatic alteration in transcription, resulting in wide-scale repression of most genes and activation of some others. This coincides with an arrest of cellular proliferation. A subset of such cells enters quiescence, a reversible non-dividing state. Here, we demonstrate that the conserved transcriptional corepressor Tup1 is critical for transcriptional repression after glucose depletion. We show that Tup1-Ssn6 binds new targets upon glucose depletion, where it remains as the cells enter the G0 phase of the cell cycle. In addition, we show that Tup1 represses a variety of glucose metabolism and transport genes. We explored how Tup1 mediated repression is accomplished and demonstrated that Tup1 coordinates with the Rpd3L complex to deacetylate H3K23. We found that Tup1 coordinates with Isw2 to affect nucleosome positions at glucose transporter HXT family genes during G0. Finally, microscopy revealed that a quarter of cells with a Tup1 deletion contain multiple DAPI puncta. Taken together, these findings demonstrate the role of Tup1 in transcriptional reprogramming in response to environmental cues leading to the quiescent state.https://doi.org/10.1371/journal.pgen.1010559 |
| spellingShingle | Thomas B Bailey Phaedra A Whitty Eric U Selker Jeffrey N McKnight Laura E McKnight Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae. PLoS Genetics |
| title | Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae. |
| title_full | Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae. |
| title_fullStr | Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae. |
| title_full_unstemmed | Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae. |
| title_short | Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae. |
| title_sort | tup1 is critical for transcriptional repression in quiescence in s cerevisiae |
| url | https://doi.org/10.1371/journal.pgen.1010559 |
| work_keys_str_mv | AT thomasbbailey tup1iscriticalfortranscriptionalrepressioninquiescenceinscerevisiae AT phaedraawhitty tup1iscriticalfortranscriptionalrepressioninquiescenceinscerevisiae AT ericuselker tup1iscriticalfortranscriptionalrepressioninquiescenceinscerevisiae AT jeffreynmcknight tup1iscriticalfortranscriptionalrepressioninquiescenceinscerevisiae AT lauraemcknight tup1iscriticalfortranscriptionalrepressioninquiescenceinscerevisiae |