In vitro Hypolipidemic and Antioxidant Effects of Leaf and Root Extracts of Taraxacum Officinale
Adipose tissue dysfunction constitutes a primary defect in obesity and might link this disease to severe chronic health problems. We aimed to evaluate the antioxidant activity of three extracts from Taraxacum officinale (dandelion) as well as their effects on mature 3T3-L1 adipocytes concerning intr...
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MDPI AG
2015-06-01
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| author | Belén García-Carrasco Raquel Fernandez-Dacosta Alberto Dávalos José M. Ordovás Arantxa Rodriguez-Casado |
| author_facet | Belén García-Carrasco Raquel Fernandez-Dacosta Alberto Dávalos José M. Ordovás Arantxa Rodriguez-Casado |
| author_sort | Belén García-Carrasco |
| collection | DOAJ |
| description | Adipose tissue dysfunction constitutes a primary defect in obesity and might link this disease to severe chronic health problems. We aimed to evaluate the antioxidant activity of three extracts from Taraxacum officinale (dandelion) as well as their effects on mature 3T3-L1 adipocytes concerning intracellular lipid accumulation and cytotoxicity, this would give indications regarding therapeutic interest of dandelion as potential anti-obesity candidate. Antioxidant activities of extracts from dandelion roots and leaves were evaluated in vitro using 1,1-diphenyl-2-picrylhyorazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) methods at the concentration range used in cellular assays (300–600 µg/mL). The influence of the extracts on mature 3T3-L1 adipocyte viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Lipid content was determined by Oil-red-O staining. The extracts showed effective antioxidant activity correlating with total flavonoid and polyphenol contents. However, the functionality level was weakly associated with the antioxidant activity. Further, our data demonstrated that mature 3T3-L1 adipocytes reduced in size and number when incubated with the extracts, which suggests a significant increase in lipolysis activity. Particularly, leaf extract and crude powdered root of dandelion reduced triglyceride accumulation in mature 3T3-L1 adipocytes to a greater extent that the extract from the root. Our study shows anti-lipogenic effects of dandelion extracts on adipocytes as well as radical scavenging and reducing activity. Importantly, along with previous results indicating that cell populations cultivated in the presence of the dandelion extracts decrease in 3T3-L1 adipogenesis capacity, these results suggests that these extracts might represent a treatment option for obesity-related diseases by affecting different processes during the adipocyte life cycle. |
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| last_indexed | 2024-12-12T22:13:26Z |
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| series | Medical Sciences |
| spelling | doaj.art-727c9471ab91406b9e25ea37a1642ee42022-12-22T00:10:10ZengMDPI AGMedical Sciences2076-32712015-06-0132385410.3390/medsci3020038medsci3020038In vitro Hypolipidemic and Antioxidant Effects of Leaf and Root Extracts of Taraxacum OfficinaleBelén García-Carrasco0Raquel Fernandez-Dacosta1Alberto Dávalos2José M. Ordovás3Arantxa Rodriguez-Casado4IMDEA Food Institute, C/Carretera de Cantoblanco 8, 28049 Madrid, SpainIMDEA Food Institute, C/Carretera de Cantoblanco 8, 28049 Madrid, SpainIMDEA Food Institute, C/Carretera de Cantoblanco 8, 28049 Madrid, SpainIMDEA Food Institute, C/Carretera de Cantoblanco 8, 28049 Madrid, SpainIMDEA Food Institute, C/Carretera de Cantoblanco 8, 28049 Madrid, SpainAdipose tissue dysfunction constitutes a primary defect in obesity and might link this disease to severe chronic health problems. We aimed to evaluate the antioxidant activity of three extracts from Taraxacum officinale (dandelion) as well as their effects on mature 3T3-L1 adipocytes concerning intracellular lipid accumulation and cytotoxicity, this would give indications regarding therapeutic interest of dandelion as potential anti-obesity candidate. Antioxidant activities of extracts from dandelion roots and leaves were evaluated in vitro using 1,1-diphenyl-2-picrylhyorazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) methods at the concentration range used in cellular assays (300–600 µg/mL). The influence of the extracts on mature 3T3-L1 adipocyte viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Lipid content was determined by Oil-red-O staining. The extracts showed effective antioxidant activity correlating with total flavonoid and polyphenol contents. However, the functionality level was weakly associated with the antioxidant activity. Further, our data demonstrated that mature 3T3-L1 adipocytes reduced in size and number when incubated with the extracts, which suggests a significant increase in lipolysis activity. Particularly, leaf extract and crude powdered root of dandelion reduced triglyceride accumulation in mature 3T3-L1 adipocytes to a greater extent that the extract from the root. Our study shows anti-lipogenic effects of dandelion extracts on adipocytes as well as radical scavenging and reducing activity. Importantly, along with previous results indicating that cell populations cultivated in the presence of the dandelion extracts decrease in 3T3-L1 adipogenesis capacity, these results suggests that these extracts might represent a treatment option for obesity-related diseases by affecting different processes during the adipocyte life cycle.http://www.mdpi.com/2076-3271/3/2/38adipocyteantioxidantcholesteroldandelionpolyphenolstriglycerides |
| spellingShingle | Belén García-Carrasco Raquel Fernandez-Dacosta Alberto Dávalos José M. Ordovás Arantxa Rodriguez-Casado In vitro Hypolipidemic and Antioxidant Effects of Leaf and Root Extracts of Taraxacum Officinale Medical Sciences adipocyte antioxidant cholesterol dandelion polyphenols triglycerides |
| title | In vitro Hypolipidemic and Antioxidant Effects of Leaf and Root Extracts of Taraxacum Officinale |
| title_full | In vitro Hypolipidemic and Antioxidant Effects of Leaf and Root Extracts of Taraxacum Officinale |
| title_fullStr | In vitro Hypolipidemic and Antioxidant Effects of Leaf and Root Extracts of Taraxacum Officinale |
| title_full_unstemmed | In vitro Hypolipidemic and Antioxidant Effects of Leaf and Root Extracts of Taraxacum Officinale |
| title_short | In vitro Hypolipidemic and Antioxidant Effects of Leaf and Root Extracts of Taraxacum Officinale |
| title_sort | in vitro hypolipidemic and antioxidant effects of leaf and root extracts of taraxacum officinale |
| topic | adipocyte antioxidant cholesterol dandelion polyphenols triglycerides |
| url | http://www.mdpi.com/2076-3271/3/2/38 |
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