The effects of inosine on clinical and histological findings after experimental spinal cord injury in rats
Inosine is a naturally occurring purine nucleoside, the effect of which was discovered only in recent decades. It has potential to prevent neuronal and glial death and can stimulate axonal outgrowth. This study evaluated the effect of inosine (400 mg per rat) administered orally two hours after expe...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2015-04-01
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| Series: | Veterinární Medicína |
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| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201504-0003_the-effects-of-inosine-on-clinical-and-histological-findings-after-experimental-spinal-cord-injury-in-rats.php |
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| author | V. Ledecky M. Kuricova T. Liptak D. Cizkova |
| author_facet | V. Ledecky M. Kuricova T. Liptak D. Cizkova |
| author_sort | V. Ledecky |
| collection | DOAJ |
| description | Inosine is a naturally occurring purine nucleoside, the effect of which was discovered only in recent decades. It has potential to prevent neuronal and glial death and can stimulate axonal outgrowth. This study evaluated the effect of inosine (400 mg per rat) administered orally two hours after experimental spinal cord injury and continuously daily for 12 days. We observed the effect of inosine on clinical and histological changes by means of measurement of recovery of hind leg motor function and urinary bladder function, frequency of spasms, neuronal profile and spinal cord tissue sparing. The rats were randomly divided into three groups, SCI-Aqua and SCI-Inosine, with daily administration of aqua for injection or inosine, and SCI-Sham group without spinal injury. The motor function of hind legs and urinary bladder function were evaluated daily for 12 days after the spinal cord injury. In the SCI-Inosine group we recorded lower incidence of spasms due to spinal cord irritation in the early postoperative period when compared to the SCI-Aqua group. We used immunohistochemistry with specific neuronal antibodies to determine the neuronal profiles and the Luxol fast blue staining method to detect the white and grey matter tissue sparing. In our study we recorded significant differences in recovery between the SCI-Aqua and SCI-Inosine group from eighth days after surgery. Moreover, the post mortem investigation of transverse spinal cord sections revealed significantly higher numbers in the SCI-Inosine group (more neurons, greater tissue sparing). According to our findings inosine accelerates the recovery of neurological functions. |
| first_indexed | 2024-04-10T07:59:43Z |
| format | Article |
| id | doaj.art-727e0e8b06b54fe48cab5deb16df5736 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:59:43Z |
| publishDate | 2015-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-727e0e8b06b54fe48cab5deb16df57362023-02-23T03:50:24ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922015-04-0160418619310.17221/8106-VETMEDvet-201504-0003The effects of inosine on clinical and histological findings after experimental spinal cord injury in ratsV. Ledecky0M. Kuricova1T. Liptak2D. Cizkova3University of Veterinary Medicine and Pharmacy, Kosice, Slovak RepublicUniversity of Veterinary Medicine and Pharmacy, Kosice, Slovak RepublicUniversity of Veterinary Medicine and Pharmacy, Kosice, Slovak RepublicInstitute of Neurobiology, Slovak Academy of Sciences, Centre of Excellence for Brain Research, Kosice, Slovak RepublicInosine is a naturally occurring purine nucleoside, the effect of which was discovered only in recent decades. It has potential to prevent neuronal and glial death and can stimulate axonal outgrowth. This study evaluated the effect of inosine (400 mg per rat) administered orally two hours after experimental spinal cord injury and continuously daily for 12 days. We observed the effect of inosine on clinical and histological changes by means of measurement of recovery of hind leg motor function and urinary bladder function, frequency of spasms, neuronal profile and spinal cord tissue sparing. The rats were randomly divided into three groups, SCI-Aqua and SCI-Inosine, with daily administration of aqua for injection or inosine, and SCI-Sham group without spinal injury. The motor function of hind legs and urinary bladder function were evaluated daily for 12 days after the spinal cord injury. In the SCI-Inosine group we recorded lower incidence of spasms due to spinal cord irritation in the early postoperative period when compared to the SCI-Aqua group. We used immunohistochemistry with specific neuronal antibodies to determine the neuronal profiles and the Luxol fast blue staining method to detect the white and grey matter tissue sparing. In our study we recorded significant differences in recovery between the SCI-Aqua and SCI-Inosine group from eighth days after surgery. Moreover, the post mortem investigation of transverse spinal cord sections revealed significantly higher numbers in the SCI-Inosine group (more neurons, greater tissue sparing). According to our findings inosine accelerates the recovery of neurological functions.https://vetmed.agriculturejournals.cz/artkey/vet-201504-0003_the-effects-of-inosine-on-clinical-and-histological-findings-after-experimental-spinal-cord-injury-in-rats.phpspinal compressionsecondary injurylocomotor functionurinary bladder functionaxonal rewiring |
| spellingShingle | V. Ledecky M. Kuricova T. Liptak D. Cizkova The effects of inosine on clinical and histological findings after experimental spinal cord injury in rats Veterinární Medicína spinal compression secondary injury locomotor function urinary bladder function axonal rewiring |
| title | The effects of inosine on clinical and histological findings after experimental spinal cord injury in rats |
| title_full | The effects of inosine on clinical and histological findings after experimental spinal cord injury in rats |
| title_fullStr | The effects of inosine on clinical and histological findings after experimental spinal cord injury in rats |
| title_full_unstemmed | The effects of inosine on clinical and histological findings after experimental spinal cord injury in rats |
| title_short | The effects of inosine on clinical and histological findings after experimental spinal cord injury in rats |
| title_sort | effects of inosine on clinical and histological findings after experimental spinal cord injury in rats |
| topic | spinal compression secondary injury locomotor function urinary bladder function axonal rewiring |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201504-0003_the-effects-of-inosine-on-clinical-and-histological-findings-after-experimental-spinal-cord-injury-in-rats.php |
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