Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells
The present study was evaluated the anticancer activity of Radix Pulsatillae ethanol extract at first by using high throughput screening HTS for herb fractions screening in concentration of 100μM to identify the active fraction and then we measured the cytotoxic effects by using morphology changes...
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| Format: | Article |
| Language: | English |
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University of Thi-Qar
2019-05-01
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| Series: | مجلة علوم ذي قار |
| Subjects: | |
| Online Access: | https://www.jsci.utq.edu.iq/index.php/main/article/view/115 |
| _version_ | 1797376495857958912 |
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| author | Ali Al-Shawi |
| author_facet | Ali Al-Shawi |
| author_sort | Ali Al-Shawi |
| collection | DOAJ |
| description |
The present study was evaluated the anticancer activity of Radix Pulsatillae ethanol extract at first by using high
throughput screening HTS for herb fractions screening in concentration of 100μM to identify the active fraction and
then we measured the cytotoxic effects by using morphology changes, and detected apoptosis by Hoechst 33258
staining. Our results showed that Radix Pulsatillae ethanol extract has a strong toxicity against A549 lung cancer cells
and low toxicity with mice normal splenocytes and requires more investigation to identify the small natural molecule
with new anticancer activity and further mechanistic for cancer pathways
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| first_indexed | 2024-03-08T19:39:27Z |
| format | Article |
| id | doaj.art-728121ba2280409a8dc2868c77262f1a |
| institution | Directory Open Access Journal |
| issn | 1991-8690 2709-0256 |
| language | English |
| last_indexed | 2024-03-08T19:39:27Z |
| publishDate | 2019-05-01 |
| publisher | University of Thi-Qar |
| record_format | Article |
| series | مجلة علوم ذي قار |
| spelling | doaj.art-728121ba2280409a8dc2868c77262f1a2023-12-25T10:26:40ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562019-05-0152Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells Ali Al-Shawi The present study was evaluated the anticancer activity of Radix Pulsatillae ethanol extract at first by using high throughput screening HTS for herb fractions screening in concentration of 100μM to identify the active fraction and then we measured the cytotoxic effects by using morphology changes, and detected apoptosis by Hoechst 33258 staining. Our results showed that Radix Pulsatillae ethanol extract has a strong toxicity against A549 lung cancer cells and low toxicity with mice normal splenocytes and requires more investigation to identify the small natural molecule with new anticancer activity and further mechanistic for cancer pathways https://www.jsci.utq.edu.iq/index.php/main/article/view/115Radix PulsatillaeLung cancerHoechst staining, |
| spellingShingle | Ali Al-Shawi Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells مجلة علوم ذي قار Radix Pulsatillae Lung cancer Hoechst staining, |
| title | Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells |
| title_full | Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells |
| title_fullStr | Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells |
| title_full_unstemmed | Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells |
| title_short | Inhibitory Effect of Ethanolic Extract Isolated from Radix Pulasatillae Seeds against Proliferation of A549 Human Lung Cancer Cells |
| title_sort | inhibitory effect of ethanolic extract isolated from radix pulasatillae seeds against proliferation of a549 human lung cancer cells |
| topic | Radix Pulsatillae Lung cancer Hoechst staining, |
| url | https://www.jsci.utq.edu.iq/index.php/main/article/view/115 |
| work_keys_str_mv | AT alialshawi inhibitoryeffectofethanolicextractisolatedfromradixpulasatillaeseedsagainstproliferationofa549humanlungcancercells |