Untargeted Metabolomics Approach for the Differentiation between <i>Panax vietnamensis</i> var. <i>vietnamensis</i> and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i>
<i>Panax vietnamensis</i> var. <i>vietnamensis</i> (PVV) and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> (PVF) both belong to <i>Panax vietnamensis</i> species and are chemically and morphologically similar, making it hard to distingu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/6/763 |
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| author | Huy Truong Nguyen Long Ke Phan Kim-Long Vu Huynh Thuc-Huy Duong Huong Thuy Le Nguyen Trang Hai-Yen Nguyen Thi Hai Yen Nguyen Phuoc Long Minh Duc Nguyen |
| author_facet | Huy Truong Nguyen Long Ke Phan Kim-Long Vu Huynh Thuc-Huy Duong Huong Thuy Le Nguyen Trang Hai-Yen Nguyen Thi Hai Yen Nguyen Phuoc Long Minh Duc Nguyen |
| author_sort | Huy Truong Nguyen |
| collection | DOAJ |
| description | <i>Panax vietnamensis</i> var. <i>vietnamensis</i> (PVV) and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> (PVF) both belong to <i>Panax vietnamensis</i> species and are chemically and morphologically similar, making it hard to distinguish for the consumer. Herein, 42 PVF and 12 PVV samples were collected in Quang Nam and Lai Chau Province, respectively, and subsequently characterized by ITSr-DNA sequence data to verify their origins. Next, untargeted metabolomics combined with multivariate statistical analysis was developed to differentiate PVV and PVF. The metabolic profiles of PVV and PVF were found to be distinct and classified well using Partial Least-Squares Discriminant Analysis (PLS-DA) in the training set. Among them, seven ginsenosides were of high abundance in PVV, while six were of high abundance in PVF. Next, the test set was used to validate 13 putative differential markers found in the training set, illustrating a complete match with the expression patterns of these ginsenosides in the training set. Finally, PLS-DA and linear Support Vector Machine models both indicated distinct ginsenoside profiles of PVV and PVF without misclassification in the test set. Conclusively, the developed untargeted metabolomics approach might serve as a powerful tool for the authentication of PVV and PVF at the metabolome level. |
| first_indexed | 2024-03-11T02:10:26Z |
| format | Article |
| id | doaj.art-7282a45f3efc4d26836e21ba8b009075 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T02:10:26Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-7282a45f3efc4d26836e21ba8b0090752023-11-18T11:35:20ZengMDPI AGMetabolites2218-19892023-06-0113676310.3390/metabo13060763Untargeted Metabolomics Approach for the Differentiation between <i>Panax vietnamensis</i> var. <i>vietnamensis</i> and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i>Huy Truong Nguyen0Long Ke Phan1Kim-Long Vu Huynh2Thuc-Huy Duong3Huong Thuy Le4Nguyen Trang Hai-Yen5Nguyen Thi Hai Yen6Nguyen Phuoc Long7Minh Duc Nguyen8Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamVietnam National Museum of Nature, Vietnam Academy of Science and Technology, Hanoi 122300, VietnamFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamDepartment of Chemistry, University of Education, Ho Chi Minh City 72700, VietnamFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, VietnamDepartment of Pharmacology and Pharmaco Genomics Research Center, Inje University College of Medicine, Busan 47392, Republic of KoreaDepartment of Pharmacology and Pharmaco Genomics Research Center, Inje University College of Medicine, Busan 47392, Republic of KoreaFaculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam<i>Panax vietnamensis</i> var. <i>vietnamensis</i> (PVV) and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> (PVF) both belong to <i>Panax vietnamensis</i> species and are chemically and morphologically similar, making it hard to distinguish for the consumer. Herein, 42 PVF and 12 PVV samples were collected in Quang Nam and Lai Chau Province, respectively, and subsequently characterized by ITSr-DNA sequence data to verify their origins. Next, untargeted metabolomics combined with multivariate statistical analysis was developed to differentiate PVV and PVF. The metabolic profiles of PVV and PVF were found to be distinct and classified well using Partial Least-Squares Discriminant Analysis (PLS-DA) in the training set. Among them, seven ginsenosides were of high abundance in PVV, while six were of high abundance in PVF. Next, the test set was used to validate 13 putative differential markers found in the training set, illustrating a complete match with the expression patterns of these ginsenosides in the training set. Finally, PLS-DA and linear Support Vector Machine models both indicated distinct ginsenoside profiles of PVV and PVF without misclassification in the test set. Conclusively, the developed untargeted metabolomics approach might serve as a powerful tool for the authentication of PVV and PVF at the metabolome level.https://www.mdpi.com/2218-1989/13/6/763Vietnamese ginseng<i>Panax vietnamensis</i> var. <i>vietnamensis</i><i>Panax vietnamensis</i> var. <i>fuscidiscus</i>untargeted metabolomicsauthentication |
| spellingShingle | Huy Truong Nguyen Long Ke Phan Kim-Long Vu Huynh Thuc-Huy Duong Huong Thuy Le Nguyen Trang Hai-Yen Nguyen Thi Hai Yen Nguyen Phuoc Long Minh Duc Nguyen Untargeted Metabolomics Approach for the Differentiation between <i>Panax vietnamensis</i> var. <i>vietnamensis</i> and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> Metabolites Vietnamese ginseng <i>Panax vietnamensis</i> var. <i>vietnamensis</i> <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> untargeted metabolomics authentication |
| title | Untargeted Metabolomics Approach for the Differentiation between <i>Panax vietnamensis</i> var. <i>vietnamensis</i> and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> |
| title_full | Untargeted Metabolomics Approach for the Differentiation between <i>Panax vietnamensis</i> var. <i>vietnamensis</i> and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> |
| title_fullStr | Untargeted Metabolomics Approach for the Differentiation between <i>Panax vietnamensis</i> var. <i>vietnamensis</i> and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> |
| title_full_unstemmed | Untargeted Metabolomics Approach for the Differentiation between <i>Panax vietnamensis</i> var. <i>vietnamensis</i> and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> |
| title_short | Untargeted Metabolomics Approach for the Differentiation between <i>Panax vietnamensis</i> var. <i>vietnamensis</i> and <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> |
| title_sort | untargeted metabolomics approach for the differentiation between i panax vietnamensis i var i vietnamensis i and i panax vietnamensis i var i fuscidiscus i |
| topic | Vietnamese ginseng <i>Panax vietnamensis</i> var. <i>vietnamensis</i> <i>Panax vietnamensis</i> var. <i>fuscidiscus</i> untargeted metabolomics authentication |
| url | https://www.mdpi.com/2218-1989/13/6/763 |
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