Melatonin protects bovine embryos from heat stress and oxygen tension and improves embryo production in vitro
The objective of this study was to determine melatonin"s ameliorating effects against heat stress and oxygen tension in developing bovine embryos in vitro. The oocytes were collected from ovaries obtained from a local abattoir, followed by in vitro maturation, fertilization, and embryo culture....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2023-09-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=3017 |
| _version_ | 1797403844710236160 |
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| author | Mustafa HITIT Josh SCHINDLER Erdogan MEMILI John J. PARRISH Abdullah KAYA |
| author_facet | Mustafa HITIT Josh SCHINDLER Erdogan MEMILI John J. PARRISH Abdullah KAYA |
| author_sort | Mustafa HITIT |
| collection | DOAJ |
| description | The objective of this study was to determine melatonin"s ameliorating effects against
heat stress and oxygen tension in developing bovine embryos in vitro. The oocytes were
collected from ovaries obtained from a local abattoir, followed by in vitro maturation,
fertilization, and embryo culture. During in vitro culture, embryos were exposed to
5% (Group I) and 20% (Group II) oxygen tension with 10-3, 10-6, and 10-9 molar (M)
melatonin, along with the control group without melatonin (Group III). Compared to the
control group, melatonin at 10-6 and 10-9 concentrations increased in vitro development
rates and decreased caspase 3/7 activity at 5% and 20% oxygen tension (P<0.01). Onehalf
of the zygotes were cultured under normal temperature (38.5ºC) during the culture
period, and the other half of the zygotes were heat stressed at 41ºC for six hours. Then
they transferred into the normal culture conditions for the rest of the period using 0, 10-
6, and 10-9 M of melatonin (Group IV). Under normal temperature (38.5ºC), melatonin
at 10-9 M was beneficial for in vitro development and DNA integrity. Under heat stress at
41ºC, melatonin at 10-6 and 10-9 M was useful for in vitro development and DNA integrity
(P<0.05). Supplementation of melatonin to embryo culture medium did not alter the
caspase 3 and 7 activities (P>0.05). In conclusion, melatonin prevents the adverse effects
of heat stress and O2 tension on preimplantation bovine embryos in vitro. |
| first_indexed | 2024-03-09T02:44:20Z |
| format | Article |
| id | doaj.art-72837d367ce143d3b5d5c83892755908 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-09T02:44:20Z |
| publishDate | 2023-09-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-72837d367ce143d3b5d5c838927559082023-12-05T21:27:25ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512023-09-0129550551110.9775/kvfd.2023.295603017Melatonin protects bovine embryos from heat stress and oxygen tension and improves embryo production in vitroMustafa HITIT0Josh SCHINDLER1Erdogan MEMILI2John J. PARRISH3Abdullah KAYA4Kastamonu University, Faculty of Veterinary Medicine, Department of Genetics, TR-37150 Kastamonu - TÜRKİYEUniversity of Wisconsin, Department of Animal and Dairy Science, Madison, WI, USADepartment of Agriculture, Nutrition and Human Ecology, Cooperative Agricultural Research Center, College of Agriculture, Food and Natural Resources, Prairie View A&M University, Prairie View, TX, USAUniversity of Wisconsin, Department of Animal and Dairy Science, Madison, WI, USAUniversity of Wisconsin, Department of Animal and Dairy Science, Madison, WI, USAThe objective of this study was to determine melatonin"s ameliorating effects against heat stress and oxygen tension in developing bovine embryos in vitro. The oocytes were collected from ovaries obtained from a local abattoir, followed by in vitro maturation, fertilization, and embryo culture. During in vitro culture, embryos were exposed to 5% (Group I) and 20% (Group II) oxygen tension with 10-3, 10-6, and 10-9 molar (M) melatonin, along with the control group without melatonin (Group III). Compared to the control group, melatonin at 10-6 and 10-9 concentrations increased in vitro development rates and decreased caspase 3/7 activity at 5% and 20% oxygen tension (P<0.01). Onehalf of the zygotes were cultured under normal temperature (38.5ºC) during the culture period, and the other half of the zygotes were heat stressed at 41ºC for six hours. Then they transferred into the normal culture conditions for the rest of the period using 0, 10- 6, and 10-9 M of melatonin (Group IV). Under normal temperature (38.5ºC), melatonin at 10-9 M was beneficial for in vitro development and DNA integrity. Under heat stress at 41ºC, melatonin at 10-6 and 10-9 M was useful for in vitro development and DNA integrity (P<0.05). Supplementation of melatonin to embryo culture medium did not alter the caspase 3 and 7 activities (P>0.05). In conclusion, melatonin prevents the adverse effects of heat stress and O2 tension on preimplantation bovine embryos in vitro.https://vetdergikafkas.org/pdf.php?id=3017dna integrity embryoheat stressmelatoninoxygen concentration |
| spellingShingle | Mustafa HITIT Josh SCHINDLER Erdogan MEMILI John J. PARRISH Abdullah KAYA Melatonin protects bovine embryos from heat stress and oxygen tension and improves embryo production in vitro Kafkas Universitesi Veteriner Fakültesi Dergisi dna integrity embryo heat stress melatonin oxygen concentration |
| title | Melatonin protects bovine embryos from heat stress and oxygen tension and improves embryo production in vitro |
| title_full | Melatonin protects bovine embryos from heat stress and oxygen tension and improves embryo production in vitro |
| title_fullStr | Melatonin protects bovine embryos from heat stress and oxygen tension and improves embryo production in vitro |
| title_full_unstemmed | Melatonin protects bovine embryos from heat stress and oxygen tension and improves embryo production in vitro |
| title_short | Melatonin protects bovine embryos from heat stress and oxygen tension and improves embryo production in vitro |
| title_sort | melatonin protects bovine embryos from heat stress and oxygen tension and improves embryo production in vitro |
| topic | dna integrity embryo heat stress melatonin oxygen concentration |
| url | https://vetdergikafkas.org/pdf.php?id=3017 |
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