Pluripotency of Wolbachia against Arboviruses: the case of yellow fever [version 2; peer review: 2 approved]
Background: Yellow fever outbreaks have re-emerged in Brazil during 2016-18, with mortality rates up to 30%. Although urban transmission has not been reported since 1942, the risk of re-urbanization of yellow fever is significant, as Aedes aegypti is present in most tropical and sub-tropical cities...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2019-04-01
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| Series: | Gates Open Research |
| Online Access: | https://gatesopenresearch.org/articles/3-161/v2 |
| _version_ | 1818118528960561152 |
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| author | Marcele Neves Rocha Myrian Morato Duarte Simone Brutman Mansur Bianca Daoud Mafra e Silva Thiago Nunes Pereira Talita Émile Ribeiro Adelino Marta Giovanetti Luis Carlos Junior Alcantara Franciele Martins Santos Victor Rodrigues de Melo Costa Mauro Martins Teixeira Felipe Campos de Melo Iani Vivian Vasconcelos Costa Luciano Andrade Moreira |
| author_facet | Marcele Neves Rocha Myrian Morato Duarte Simone Brutman Mansur Bianca Daoud Mafra e Silva Thiago Nunes Pereira Talita Émile Ribeiro Adelino Marta Giovanetti Luis Carlos Junior Alcantara Franciele Martins Santos Victor Rodrigues de Melo Costa Mauro Martins Teixeira Felipe Campos de Melo Iani Vivian Vasconcelos Costa Luciano Andrade Moreira |
| author_sort | Marcele Neves Rocha |
| collection | DOAJ |
| description | Background: Yellow fever outbreaks have re-emerged in Brazil during 2016-18, with mortality rates up to 30%. Although urban transmission has not been reported since 1942, the risk of re-urbanization of yellow fever is significant, as Aedes aegypti is present in most tropical and sub-tropical cities in the World and still remains the main vector of urban YFV. Although the YFV vaccine is safe and effective, it does not always reach populations at greatest risk of infection and there is an acknowledged global shortage of vaccine supply. The introgression of Wolbachia bacteria into Ae. aegypti mosquito populations is being trialed in several countries (www.worldmosquito.org) as a biocontrol method against dengue, Zika and chikungunya. Here, we studied the ability of Wolbachia to reduce the transmission potential of Ae. aegypti mosquitoes for Yellow fever virus (YFV). Methods: Two recently isolated YFV (primate and human) were used to challenge field-derived wild-type and Wolbachia-infected (wMel +) Ae. aegypti mosquitoes. The YFV infection status was followed for 7, 14 and 21 days post-oral feeding (dpf). The YFV transmission potential of mosquitoes was evaluated via nano-injection of saliva into uninfected mosquitoes or by inoculation in mice. Results: We found that Wolbachia was able to significantly reduce the prevalence of mosquitoes with YFV infected heads and thoraces for both viral isolates. Furthermore, analyses of mosquito saliva, through indirect injection into naïve mosquitoes or via interferon-deficient mouse model, indicated Wolbachia was associated with profound reduction in the YFV transmission potential of mosquitoes (14dpf). Conclusions: Our results suggest that Wolbachia introgression could be used as a complementary strategy for prevention of urban yellow fever transmission, along with the human vaccination program. |
| first_indexed | 2024-12-11T04:55:45Z |
| format | Article |
| id | doaj.art-72843c76fcff48c7ab134246e0a08c8f |
| institution | Directory Open Access Journal |
| issn | 2572-4754 |
| language | English |
| last_indexed | 2024-12-11T04:55:45Z |
| publishDate | 2019-04-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | Gates Open Research |
| spelling | doaj.art-72843c76fcff48c7ab134246e0a08c8f2022-12-22T01:20:16ZengF1000 Research LtdGates Open Research2572-47542019-04-01310.12688/gatesopenres.12903.214075Pluripotency of Wolbachia against Arboviruses: the case of yellow fever [version 2; peer review: 2 approved]Marcele Neves Rocha0Myrian Morato Duarte1Simone Brutman Mansur2Bianca Daoud Mafra e Silva3Thiago Nunes Pereira4Talita Émile Ribeiro Adelino5Marta Giovanetti6Luis Carlos Junior Alcantara7Franciele Martins Santos8Victor Rodrigues de Melo Costa9Mauro Martins Teixeira10Felipe Campos de Melo Iani11Vivian Vasconcelos Costa12Luciano Andrade Moreira13Mosquitos Vetores, IRR, Fundação Oswaldo Cruz, Belo Horizonte, MG, BrazilServiço de Virologia e Riquetsioses, Fundação Ezequiel Dias-LACEN, Belo Horizonte, MG, BrazilMosquitos Vetores, IRR, Fundação Oswaldo Cruz, Belo Horizonte, MG, BrazilMosquitos Vetores, IRR, Fundação Oswaldo Cruz, Belo Horizonte, MG, BrazilMosquitos Vetores, IRR, Fundação Oswaldo Cruz, Belo Horizonte, MG, BrazilServiço de Virologia e Riquetsioses, Fundação Ezequiel Dias-LACEN, Belo Horizonte, MG, BrazilLaboratório de Flavivírus, IOC, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, BrazilLaboratório de Flavivírus, IOC, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, BrazilCentro de Pesquisa e Desenvolvimento de Fármacos, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilCentro de Pesquisa e Desenvolvimento de Fármacos, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilCentro de Pesquisa e Desenvolvimento de Fármacos, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilServiço de Virologia e Riquetsioses, Fundação Ezequiel Dias-LACEN, Belo Horizonte, MG, BrazilCentro de Pesquisa e Desenvolvimento de Fármacos, Universidade Federal de Minas Gerais, Belo Horizonte, MG, BrazilMosquitos Vetores, IRR, Fundação Oswaldo Cruz, Belo Horizonte, MG, BrazilBackground: Yellow fever outbreaks have re-emerged in Brazil during 2016-18, with mortality rates up to 30%. Although urban transmission has not been reported since 1942, the risk of re-urbanization of yellow fever is significant, as Aedes aegypti is present in most tropical and sub-tropical cities in the World and still remains the main vector of urban YFV. Although the YFV vaccine is safe and effective, it does not always reach populations at greatest risk of infection and there is an acknowledged global shortage of vaccine supply. The introgression of Wolbachia bacteria into Ae. aegypti mosquito populations is being trialed in several countries (www.worldmosquito.org) as a biocontrol method against dengue, Zika and chikungunya. Here, we studied the ability of Wolbachia to reduce the transmission potential of Ae. aegypti mosquitoes for Yellow fever virus (YFV). Methods: Two recently isolated YFV (primate and human) were used to challenge field-derived wild-type and Wolbachia-infected (wMel +) Ae. aegypti mosquitoes. The YFV infection status was followed for 7, 14 and 21 days post-oral feeding (dpf). The YFV transmission potential of mosquitoes was evaluated via nano-injection of saliva into uninfected mosquitoes or by inoculation in mice. Results: We found that Wolbachia was able to significantly reduce the prevalence of mosquitoes with YFV infected heads and thoraces for both viral isolates. Furthermore, analyses of mosquito saliva, through indirect injection into naïve mosquitoes or via interferon-deficient mouse model, indicated Wolbachia was associated with profound reduction in the YFV transmission potential of mosquitoes (14dpf). Conclusions: Our results suggest that Wolbachia introgression could be used as a complementary strategy for prevention of urban yellow fever transmission, along with the human vaccination program.https://gatesopenresearch.org/articles/3-161/v2 |
| spellingShingle | Marcele Neves Rocha Myrian Morato Duarte Simone Brutman Mansur Bianca Daoud Mafra e Silva Thiago Nunes Pereira Talita Émile Ribeiro Adelino Marta Giovanetti Luis Carlos Junior Alcantara Franciele Martins Santos Victor Rodrigues de Melo Costa Mauro Martins Teixeira Felipe Campos de Melo Iani Vivian Vasconcelos Costa Luciano Andrade Moreira Pluripotency of Wolbachia against Arboviruses: the case of yellow fever [version 2; peer review: 2 approved] Gates Open Research |
| title | Pluripotency of Wolbachia against Arboviruses: the case of yellow fever [version 2; peer review: 2 approved] |
| title_full | Pluripotency of Wolbachia against Arboviruses: the case of yellow fever [version 2; peer review: 2 approved] |
| title_fullStr | Pluripotency of Wolbachia against Arboviruses: the case of yellow fever [version 2; peer review: 2 approved] |
| title_full_unstemmed | Pluripotency of Wolbachia against Arboviruses: the case of yellow fever [version 2; peer review: 2 approved] |
| title_short | Pluripotency of Wolbachia against Arboviruses: the case of yellow fever [version 2; peer review: 2 approved] |
| title_sort | pluripotency of wolbachia against arboviruses the case of yellow fever version 2 peer review 2 approved |
| url | https://gatesopenresearch.org/articles/3-161/v2 |
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