Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research
The increase in bacterial resistance to antibiotics in recent years demands innovative strategies for the detection and combating of biofilms, which are notoriously resilient. Biofilms, particularly those on contact lenses, can lead to biofilm-related infections (e.g., conjunctivitis and keratitis),...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/5/2653 |
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| author | Igor Buzalewicz Aleksandra Kaczorowska Wojciech Fijałkowski Aleksandra Pietrowska Anna Karolina Matczuk Halina Podbielska Alina Wieliczko Wojciech Witkiewicz Natalia Jędruchniewicz |
| author_facet | Igor Buzalewicz Aleksandra Kaczorowska Wojciech Fijałkowski Aleksandra Pietrowska Anna Karolina Matczuk Halina Podbielska Alina Wieliczko Wojciech Witkiewicz Natalia Jędruchniewicz |
| author_sort | Igor Buzalewicz |
| collection | DOAJ |
| description | The increase in bacterial resistance to antibiotics in recent years demands innovative strategies for the detection and combating of biofilms, which are notoriously resilient. Biofilms, particularly those on contact lenses, can lead to biofilm-related infections (e.g., conjunctivitis and keratitis), posing a significant risk to patients. Non-destructive and non-contact sensing techniques are essential in addressing this threat. Digital holographic tomography emerges as a promising solution. This allows for the 3D reconstruction of the refractive index distribution in biological samples, enabling label-free visualization and the quantitative analysis of biofilms. This tool provides insight into the dynamics of biofilm formation and maturation on the surface of transparent materials. Applying digital holographic tomography for biofilm examination has the potential to advance our ability to combat the antibiotic bacterial resistance crisis. A recent study focused on characterizing biofilm formation and maturation on six soft contact lens materials (three silicone hydrogels, three hydrogels), with a particular emphasis on <i>Staphylococcus epidermis</i> and <i>Pseudomonas aeruginosa</i>, both common culprits in ocular infections. The results revealed species- and time-dependent variations in the refractive indexes and volumes of biofilms, shedding light on cell dynamics, cell death, and contact lens material-related factors. The use of digital holographic tomography enables the quantitative analysis of biofilm dynamics, providing us with a better understanding and characterization of bacterial biofilms. |
| first_indexed | 2024-04-25T00:29:01Z |
| format | Article |
| id | doaj.art-7287fd596cbd4ab99cc1c959e9a15ade |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-25T00:29:01Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7287fd596cbd4ab99cc1c959e9a15ade2024-03-12T16:45:49ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01255265310.3390/ijms25052653Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm ResearchIgor Buzalewicz0Aleksandra Kaczorowska1Wojciech Fijałkowski2Aleksandra Pietrowska3Anna Karolina Matczuk4Halina Podbielska5Alina Wieliczko6Wojciech Witkiewicz7Natalia Jędruchniewicz8Department of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology, 50-370 Wroclaw, PolandDepartment of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology, 50-370 Wroclaw, PolandLipoTech Ltd., Liszki 536, 32-060 Liszki, PolandDepartment of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology, 50-370 Wroclaw, PolandDepartment of Pathology, Division of Microbiology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 31 C.K. Norwida St., 51-375 Wroclaw, PolandDepartment of Biomedical Engineering, Faculty of Fundamental Problems of Technology, Wroclaw University of Science and Technology, 50-370 Wroclaw, PolandDepartment of Epizootiology and Veterinary Administration with Clinic of Infectious Diseases, Wroclaw University of Environmental and Life Sciences, 45 Grunwaldzki Square, 50-366 Wroclaw, PolandResearch and Development Centre, Regional Specialist Hospital in Wroclaw, 73A H. M. Kamienskiego St., 51-124 Wroclaw, PolandResearch and Development Centre, Regional Specialist Hospital in Wroclaw, 73A H. M. Kamienskiego St., 51-124 Wroclaw, PolandThe increase in bacterial resistance to antibiotics in recent years demands innovative strategies for the detection and combating of biofilms, which are notoriously resilient. Biofilms, particularly those on contact lenses, can lead to biofilm-related infections (e.g., conjunctivitis and keratitis), posing a significant risk to patients. Non-destructive and non-contact sensing techniques are essential in addressing this threat. Digital holographic tomography emerges as a promising solution. This allows for the 3D reconstruction of the refractive index distribution in biological samples, enabling label-free visualization and the quantitative analysis of biofilms. This tool provides insight into the dynamics of biofilm formation and maturation on the surface of transparent materials. Applying digital holographic tomography for biofilm examination has the potential to advance our ability to combat the antibiotic bacterial resistance crisis. A recent study focused on characterizing biofilm formation and maturation on six soft contact lens materials (three silicone hydrogels, three hydrogels), with a particular emphasis on <i>Staphylococcus epidermis</i> and <i>Pseudomonas aeruginosa</i>, both common culprits in ocular infections. The results revealed species- and time-dependent variations in the refractive indexes and volumes of biofilms, shedding light on cell dynamics, cell death, and contact lens material-related factors. The use of digital holographic tomography enables the quantitative analysis of biofilm dynamics, providing us with a better understanding and characterization of bacterial biofilms.https://www.mdpi.com/1422-0067/25/5/2653digital holographic tomographyquantitative phase imagingbacterial biofilms<i>P. aeruginosa</i><i>S. epidermidis</i>dynamics of biofilm formation |
| spellingShingle | Igor Buzalewicz Aleksandra Kaczorowska Wojciech Fijałkowski Aleksandra Pietrowska Anna Karolina Matczuk Halina Podbielska Alina Wieliczko Wojciech Witkiewicz Natalia Jędruchniewicz Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research International Journal of Molecular Sciences digital holographic tomography quantitative phase imaging bacterial biofilms <i>P. aeruginosa</i> <i>S. epidermidis</i> dynamics of biofilm formation |
| title | Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research |
| title_full | Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research |
| title_fullStr | Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research |
| title_full_unstemmed | Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research |
| title_short | Quantifying the Dynamics of Bacterial Biofilm Formation on the Surface of Soft Contact Lens Materials Using Digital Holographic Tomography to Advance Biofilm Research |
| title_sort | quantifying the dynamics of bacterial biofilm formation on the surface of soft contact lens materials using digital holographic tomography to advance biofilm research |
| topic | digital holographic tomography quantitative phase imaging bacterial biofilms <i>P. aeruginosa</i> <i>S. epidermidis</i> dynamics of biofilm formation |
| url | https://www.mdpi.com/1422-0067/25/5/2653 |
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