Phylogenetic analysis highlights the role of older people in the transmission of HIV-1 in Fuyang, Anhui Province, China
Abstract Background The proportion of older HIV-1 infected people in China has increased rapidly in recent years. Elucidation of the transmission characteristics of this high-risk population subgroup is helpful for the development of tailored interventions. Methods A phylogenetic analysis was perfor...
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| Language: | English |
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BMC
2019-06-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | http://link.springer.com/article/10.1186/s12879-019-4187-9 |
| _version_ | 1818846408317337600 |
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| author | Jianjun Wu Yu Zhang Yuelan Shen Xiaolin Wang Hui Xing Xiaohui Yang Xinping Ding Bing Hu Hanping Li Jingwan Han Jingyun Li Bin Su Yongjian Liu Lin Li |
| author_facet | Jianjun Wu Yu Zhang Yuelan Shen Xiaolin Wang Hui Xing Xiaohui Yang Xinping Ding Bing Hu Hanping Li Jingwan Han Jingyun Li Bin Su Yongjian Liu Lin Li |
| author_sort | Jianjun Wu |
| collection | DOAJ |
| description | Abstract Background The proportion of older HIV-1 infected people in China has increased rapidly in recent years. Elucidation of the transmission characteristics of this high-risk population subgroup is helpful for the development of tailored interventions. Methods A phylogenetic analysis was performed that uses available HIV-1 pol sequences amplified with nested RT-PCR from plasma samples of all newly diagnosed participants spanning from October 2017 to September 2018 in Fuyang, Anhui Province. Transmission clusters were identified as two or more sequences that shared a corresponding node with an aLRT-SH value ≥90 in the maximum-likelihood phylogenetic tree and had an overall mean genetic distance of ≤1.5%. A local transmission cluster was defined as a cluster that had more than 80% of its sequences from Fuyang. The role of older people in local HIV-1 transmission was determined using an integration of molecular and demographic data. Results Of 362 available sequences, 14 subtypes, and 28 local transmission clusters were identified. It was found that the proportion of older people in the local transmission cluster (69/77, 89.61%) was much higher than that of younger people (46/114, 40.35%) (χ2 test, P < 0.001). In the pretreatment drug resistance analysis, the proportion of sequences with PDRMs in the local transmission cluster was not significantly different between the older people group (57.14%, 4/7) and non-old-aged group (11.11%, 1/9) (Fisher’s exact test, P > 0.05). Conclusion By combining phylogenetic analyses with demographic data, more detailed information was provided about the local transmission structure in Fuyang. These findings suggested that older people play an important role in local transmission, and more tailored interventions for this population subgroup are urgently needed. |
| first_indexed | 2024-12-19T05:45:05Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-19T05:45:05Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-728b04a835aa469c893d03acc47342712022-12-21T20:33:52ZengBMCBMC Infectious Diseases1471-23342019-06-011911910.1186/s12879-019-4187-9Phylogenetic analysis highlights the role of older people in the transmission of HIV-1 in Fuyang, Anhui Province, ChinaJianjun Wu0Yu Zhang1Yuelan Shen2Xiaolin Wang3Hui Xing4Xiaohui Yang5Xinping Ding6Bing Hu7Hanping Li8Jingwan Han9Jingyun Li10Bin Su11Yongjian Liu12Lin Li13Anhui Provincial Center for Disease Control and PreventionDepartment of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyAnhui Provincial Center for Disease Control and PreventionDepartment of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyState Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and PreventionFuyang Center for Disease Control and PreventionFuyang Center for Disease Control and PreventionFuyang Center for Disease Control and PreventionDepartment of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyDepartment of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyDepartment of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyAnhui Provincial Center for Disease Control and PreventionDepartment of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyDepartment of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and EpidemiologyAbstract Background The proportion of older HIV-1 infected people in China has increased rapidly in recent years. Elucidation of the transmission characteristics of this high-risk population subgroup is helpful for the development of tailored interventions. Methods A phylogenetic analysis was performed that uses available HIV-1 pol sequences amplified with nested RT-PCR from plasma samples of all newly diagnosed participants spanning from October 2017 to September 2018 in Fuyang, Anhui Province. Transmission clusters were identified as two or more sequences that shared a corresponding node with an aLRT-SH value ≥90 in the maximum-likelihood phylogenetic tree and had an overall mean genetic distance of ≤1.5%. A local transmission cluster was defined as a cluster that had more than 80% of its sequences from Fuyang. The role of older people in local HIV-1 transmission was determined using an integration of molecular and demographic data. Results Of 362 available sequences, 14 subtypes, and 28 local transmission clusters were identified. It was found that the proportion of older people in the local transmission cluster (69/77, 89.61%) was much higher than that of younger people (46/114, 40.35%) (χ2 test, P < 0.001). In the pretreatment drug resistance analysis, the proportion of sequences with PDRMs in the local transmission cluster was not significantly different between the older people group (57.14%, 4/7) and non-old-aged group (11.11%, 1/9) (Fisher’s exact test, P > 0.05). Conclusion By combining phylogenetic analyses with demographic data, more detailed information was provided about the local transmission structure in Fuyang. These findings suggested that older people play an important role in local transmission, and more tailored interventions for this population subgroup are urgently needed.http://link.springer.com/article/10.1186/s12879-019-4187-9HIV-1Older peopleTransmission clustersPhylogeneticEpidemiology |
| spellingShingle | Jianjun Wu Yu Zhang Yuelan Shen Xiaolin Wang Hui Xing Xiaohui Yang Xinping Ding Bing Hu Hanping Li Jingwan Han Jingyun Li Bin Su Yongjian Liu Lin Li Phylogenetic analysis highlights the role of older people in the transmission of HIV-1 in Fuyang, Anhui Province, China BMC Infectious Diseases HIV-1 Older people Transmission clusters Phylogenetic Epidemiology |
| title | Phylogenetic analysis highlights the role of older people in the transmission of HIV-1 in Fuyang, Anhui Province, China |
| title_full | Phylogenetic analysis highlights the role of older people in the transmission of HIV-1 in Fuyang, Anhui Province, China |
| title_fullStr | Phylogenetic analysis highlights the role of older people in the transmission of HIV-1 in Fuyang, Anhui Province, China |
| title_full_unstemmed | Phylogenetic analysis highlights the role of older people in the transmission of HIV-1 in Fuyang, Anhui Province, China |
| title_short | Phylogenetic analysis highlights the role of older people in the transmission of HIV-1 in Fuyang, Anhui Province, China |
| title_sort | phylogenetic analysis highlights the role of older people in the transmission of hiv 1 in fuyang anhui province china |
| topic | HIV-1 Older people Transmission clusters Phylogenetic Epidemiology |
| url | http://link.springer.com/article/10.1186/s12879-019-4187-9 |
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