A test of metabarcoding for Early Detection and Rapid Response monitoring for non-native forest pest beetles (Coleoptera)
In response to the threat of introductions of non-native forest insects, the Early Detection and Rapid Response (EDRR) program in Alaska monitors for arrivals of non-native insects, an effort that is limited by the time required to process samples using morphological methods. We compared conventiona...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2019-11-01
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| Series: | Research Ideas and Outcomes |
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| Online Access: | https://riojournal.com/article/48536/download/pdf/ |
| _version_ | 1811247631519711232 |
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| author | Matthew Bowser Stephen Burr Isaac Davis Garret Dubois Elizabeth Graham Jason Moan Steven Swenson |
| author_facet | Matthew Bowser Stephen Burr Isaac Davis Garret Dubois Elizabeth Graham Jason Moan Steven Swenson |
| author_sort | Matthew Bowser |
| collection | DOAJ |
| description | In response to the threat of introductions of non-native forest insects, the Early Detection and Rapid Response (EDRR) program in Alaska monitors for arrivals of non-native insects, an effort that is limited by the time required to process samples using morphological methods. We compared conventional methods of processing EDRR traps with metabarcoding methods for processing the same samples.
We deployed Lindgren funnel traps at three points of entry in Alaska using standard EDRR methods and trap samples were later processed using routine sorting and identification based on morphology. Samples were then processed using High Throughput Sequencing (HTS) metabarcoding methods. In three samples bycatch was included and in three samples non-native species were added.
Morophological and HTS methods yielded generally similar results for scolytine and cerambycid beetle assemblages, but HTS provided more species-resolution identifications (46 species) than morphological methods (4 species plus the 3 non-native species known a priori). None of the non-native species were detected by HTS. Including bycatch did not appear to hinder identifications of scolytine and cerambycid beetles by HTS. From among the bycatch, two Palearctic species adventive to North America, Placusa incompleta Sjöberg, 1934 and Hydrophoria lancifer (Harris, 1780), are newly reported from Alaska.
We do not recommend replacing our current morphological monitoring methods with HTS methods because we believe that we would be more likely to detect known non-native pest species using morphology. However, we would use HTS to increase our sample size without greatly increasing time required to process samples. We would also recommend HTS methods for surveillance monitoring where the set of target taxa is not limited to known pest species. |
| first_indexed | 2024-04-12T15:13:10Z |
| format | Article |
| id | doaj.art-7294aba303fd49a790d040ea35882a8f |
| institution | Directory Open Access Journal |
| issn | 2367-7163 |
| language | English |
| last_indexed | 2024-04-12T15:13:10Z |
| publishDate | 2019-11-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Research Ideas and Outcomes |
| spelling | doaj.art-7294aba303fd49a790d040ea35882a8f2022-12-22T03:27:42ZengPensoft PublishersResearch Ideas and Outcomes2367-71632019-11-01511910.3897/rio.5.e4853648536A test of metabarcoding for Early Detection and Rapid Response monitoring for non-native forest pest beetles (Coleoptera)Matthew Bowser0Stephen Burr1Isaac Davis2Garret Dubois3Elizabeth Graham4Jason Moan5Steven Swenson6U.S. Fish & Wildlife Service, Kenai National Wildlife RefugeUSDA Forest ServiceUSDA Forest ServiceUSDA Forest ServiceUSDA Forest ServiceAlaska Division of ForestryUSDA Forest ServiceIn response to the threat of introductions of non-native forest insects, the Early Detection and Rapid Response (EDRR) program in Alaska monitors for arrivals of non-native insects, an effort that is limited by the time required to process samples using morphological methods. We compared conventional methods of processing EDRR traps with metabarcoding methods for processing the same samples. We deployed Lindgren funnel traps at three points of entry in Alaska using standard EDRR methods and trap samples were later processed using routine sorting and identification based on morphology. Samples were then processed using High Throughput Sequencing (HTS) metabarcoding methods. In three samples bycatch was included and in three samples non-native species were added. Morophological and HTS methods yielded generally similar results for scolytine and cerambycid beetle assemblages, but HTS provided more species-resolution identifications (46 species) than morphological methods (4 species plus the 3 non-native species known a priori). None of the non-native species were detected by HTS. Including bycatch did not appear to hinder identifications of scolytine and cerambycid beetles by HTS. From among the bycatch, two Palearctic species adventive to North America, Placusa incompleta Sjöberg, 1934 and Hydrophoria lancifer (Harris, 1780), are newly reported from Alaska. We do not recommend replacing our current morphological monitoring methods with HTS methods because we believe that we would be more likely to detect known non-native pest species using morphology. However, we would use HTS to increase our sample size without greatly increasing time required to process samples. We would also recommend HTS methods for surveillance monitoring where the set of target taxa is not limited to known pest species.https://riojournal.com/article/48536/download/pdf/ScolytinaeCerambycidaebiomonitoringHigh-T |
| spellingShingle | Matthew Bowser Stephen Burr Isaac Davis Garret Dubois Elizabeth Graham Jason Moan Steven Swenson A test of metabarcoding for Early Detection and Rapid Response monitoring for non-native forest pest beetles (Coleoptera) Research Ideas and Outcomes Scolytinae Cerambycidae biomonitoring High-T |
| title | A test of metabarcoding for Early Detection and Rapid Response monitoring for non-native forest pest beetles (Coleoptera) |
| title_full | A test of metabarcoding for Early Detection and Rapid Response monitoring for non-native forest pest beetles (Coleoptera) |
| title_fullStr | A test of metabarcoding for Early Detection and Rapid Response monitoring for non-native forest pest beetles (Coleoptera) |
| title_full_unstemmed | A test of metabarcoding for Early Detection and Rapid Response monitoring for non-native forest pest beetles (Coleoptera) |
| title_short | A test of metabarcoding for Early Detection and Rapid Response monitoring for non-native forest pest beetles (Coleoptera) |
| title_sort | test of metabarcoding for early detection and rapid response monitoring for non native forest pest beetles coleoptera |
| topic | Scolytinae Cerambycidae biomonitoring High-T |
| url | https://riojournal.com/article/48536/download/pdf/ |
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