An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue
Mössbauer spectroscopy is not routinely used for the determination of the concentration of iron. However, as this method does not need any pre-treatment of samples before measurements, it may be of extreme importance for the assessment of iron in samples, which can then be used for further investiga...
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| Format: | Article |
| Language: | English |
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Sciendo
2017-06-01
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| Series: | Nukleonika |
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| Online Access: | https://doi.org/10.1515/nuka-2017-0024 |
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| author | Rzepecka Patrycja Duda Przemysław Giebułtowicz Joanna Sochacka Małgorzata Friedman Andrzej Gałązka-Friedman Jolanta |
| author_facet | Rzepecka Patrycja Duda Przemysław Giebułtowicz Joanna Sochacka Małgorzata Friedman Andrzej Gałązka-Friedman Jolanta |
| author_sort | Rzepecka Patrycja |
| collection | DOAJ |
| description | Mössbauer spectroscopy is not routinely used for the determination of the concentration of iron. However, as this method does not need any pre-treatment of samples before measurements, it may be of extreme importance for the assessment of iron in samples, which can then be used for further investigations. Biological samples are a good example, however, as the concentrations of iron are very low in these, it is important to exclude possible artefacts from the background spectrum related to iron present in the counter and cryostat windows. The aim of this study was to compare two methods of determination of the amounts of iron in investigated sample: one, in which the background spectrum was subtracted from the sample spectrum measured, and the other, in which the obtained non-elaborated spectrum was fitted with two doublets - a doublet for the measured sample and a doublet for the background spectrum. Three samples containing known amounts of natural iron (400, 800 and 1600 μg) and a sample of lyophilized human brain tissue obtained from globus pallidus were assessed. Both methods led to the creation of a very good calibration curve with a correlation coefficient of 0.99. Although both methods gave similar results for the concentration of iron in the sample, the subtraction of the background spectrum had a significantly lower error of the final result. |
| first_indexed | 2024-04-11T16:21:01Z |
| format | Article |
| id | doaj.art-7296a7481ae549d9b6c2980e295b6408 |
| institution | Directory Open Access Journal |
| issn | 0029-5922 |
| language | English |
| last_indexed | 2024-04-11T16:21:01Z |
| publishDate | 2017-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Nukleonika |
| spelling | doaj.art-7296a7481ae549d9b6c2980e295b64082022-12-22T04:14:20ZengSciendoNukleonika0029-59222017-06-0162215916310.1515/nuka-2017-0024nuka-2017-0024An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissueRzepecka Patrycja0Duda Przemysław1Giebułtowicz Joanna2Sochacka Małgorzata3Friedman Andrzej4Gałązka-Friedman Jolanta5Faculty of Physics, Warsaw University of Technology, 75 Koszykowa Str., 00-662 Warsaw, PolandFaculty of Physics, Warsaw University of Technology, 75 Koszykowa Str., 00-662 Warsaw, PolandFaculty of Pharmacy, Medical University of Warsaw, Warsaw, PolandFaculty of Pharmacy, Medical University of Warsaw, Warsaw, PolandDepartment of Neurology, Faculty of Health Science, Medical University of Warsaw, Warsaw, PolandFaculty of Physics, Warsaw University of Technology, 75 Koszykowa Str., 00-662 Warsaw, PolandMössbauer spectroscopy is not routinely used for the determination of the concentration of iron. However, as this method does not need any pre-treatment of samples before measurements, it may be of extreme importance for the assessment of iron in samples, which can then be used for further investigations. Biological samples are a good example, however, as the concentrations of iron are very low in these, it is important to exclude possible artefacts from the background spectrum related to iron present in the counter and cryostat windows. The aim of this study was to compare two methods of determination of the amounts of iron in investigated sample: one, in which the background spectrum was subtracted from the sample spectrum measured, and the other, in which the obtained non-elaborated spectrum was fitted with two doublets - a doublet for the measured sample and a doublet for the background spectrum. Three samples containing known amounts of natural iron (400, 800 and 1600 μg) and a sample of lyophilized human brain tissue obtained from globus pallidus were assessed. Both methods led to the creation of a very good calibration curve with a correlation coefficient of 0.99. Although both methods gave similar results for the concentration of iron in the sample, the subtraction of the background spectrum had a significantly lower error of the final result.https://doi.org/10.1515/nuka-2017-0024mössbauer spectroscopybackground spectrumiron concentrationbrain tissuecalibration curve |
| spellingShingle | Rzepecka Patrycja Duda Przemysław Giebułtowicz Joanna Sochacka Małgorzata Friedman Andrzej Gałązka-Friedman Jolanta An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue Nukleonika mössbauer spectroscopy background spectrum iron concentration brain tissue calibration curve |
| title | An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue |
| title_full | An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue |
| title_fullStr | An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue |
| title_full_unstemmed | An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue |
| title_short | An example of the application of Mössbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue |
| title_sort | example of the application of mossbauer spectroscopy for determination of concentration of iron in lyophilized brain tissue |
| topic | mössbauer spectroscopy background spectrum iron concentration brain tissue calibration curve |
| url | https://doi.org/10.1515/nuka-2017-0024 |
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