ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6
Background: ADCK2 is a member of the AarF domain-containing kinase family, which consists of five members, and has been shown to play a role in CoQ metabolism. However, ADCKs have also been connected to cancer cell survival, proliferation and motility. In this study, we investigated the role of ADCK...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/14/4/1071 |
| _version_ | 1797482077655924736 |
|---|---|
| author | Marlene Vierthaler Qian Sun Yiman Wang Tamara Steinfass Juliane Poelchen Thomas Hielscher Daniel Novak Viktor Umansky Jochen Utikal |
| author_facet | Marlene Vierthaler Qian Sun Yiman Wang Tamara Steinfass Juliane Poelchen Thomas Hielscher Daniel Novak Viktor Umansky Jochen Utikal |
| author_sort | Marlene Vierthaler |
| collection | DOAJ |
| description | Background: ADCK2 is a member of the AarF domain-containing kinase family, which consists of five members, and has been shown to play a role in CoQ metabolism. However, ADCKs have also been connected to cancer cell survival, proliferation and motility. In this study, we investigated the role of ADCK2 in melanoma. Methods: The effect of ADCK2 on melanoma cell motility was evaluated by a scratch assay and a transwell invasion assay upon siRNA-mediated knockdown or stable overexpression of ADCK2. Results: We found that high levels of intratumoral ADCK2 and MYL6 are associated with a higher survival rate in melanoma patients. Knocking down ADCK2 resulted in enhanced cell migration of melanoma cells. Moreover, ADCK2-knockdown cells adopted a more dedifferentiated phenotype. A gene expression array revealed that the expression of ADCK2 correlated with the expressions of MYL6 and RAB2A. Knocking down MYL6 in ADCK2-overexpressing cells could abrogate the effect of ADCK2 overexpression and thus confirm the functional connection between ADCK2 and MYL6. Conclusion: ADCK2 affects melanoma cell motility, most probably via MYL6. Our results allow the conclusion that ADCK2 could act as a tumor suppressor in melanoma. |
| first_indexed | 2024-03-09T22:23:09Z |
| format | Article |
| id | doaj.art-7299160abc454fd4a611e43652e62608 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-09T22:23:09Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-7299160abc454fd4a611e43652e626082023-11-23T19:10:57ZengMDPI AGCancers2072-66942022-02-01144107110.3390/cancers14041071ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6Marlene Vierthaler0Qian Sun1Yiman Wang2Tamara Steinfass3Juliane Poelchen4Thomas Hielscher5Daniel Novak6Viktor Umansky7Jochen Utikal8Skin Cancer Unit, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanySkin Cancer Unit, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanySkin Cancer Unit, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanySkin Cancer Unit, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanySkin Cancer Unit, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanyBiostatistik, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanySkin Cancer Unit, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanySkin Cancer Unit, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanySkin Cancer Unit, German Cancer Research Center (DKFZ), 69120 Heidelberg, GermanyBackground: ADCK2 is a member of the AarF domain-containing kinase family, which consists of five members, and has been shown to play a role in CoQ metabolism. However, ADCKs have also been connected to cancer cell survival, proliferation and motility. In this study, we investigated the role of ADCK2 in melanoma. Methods: The effect of ADCK2 on melanoma cell motility was evaluated by a scratch assay and a transwell invasion assay upon siRNA-mediated knockdown or stable overexpression of ADCK2. Results: We found that high levels of intratumoral ADCK2 and MYL6 are associated with a higher survival rate in melanoma patients. Knocking down ADCK2 resulted in enhanced cell migration of melanoma cells. Moreover, ADCK2-knockdown cells adopted a more dedifferentiated phenotype. A gene expression array revealed that the expression of ADCK2 correlated with the expressions of MYL6 and RAB2A. Knocking down MYL6 in ADCK2-overexpressing cells could abrogate the effect of ADCK2 overexpression and thus confirm the functional connection between ADCK2 and MYL6. Conclusion: ADCK2 affects melanoma cell motility, most probably via MYL6. Our results allow the conclusion that ADCK2 could act as a tumor suppressor in melanoma.https://www.mdpi.com/2072-6694/14/4/1071melanomaADCK2MYL6motilitycancer |
| spellingShingle | Marlene Vierthaler Qian Sun Yiman Wang Tamara Steinfass Juliane Poelchen Thomas Hielscher Daniel Novak Viktor Umansky Jochen Utikal ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6 Cancers melanoma ADCK2 MYL6 motility cancer |
| title | ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6 |
| title_full | ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6 |
| title_fullStr | ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6 |
| title_full_unstemmed | ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6 |
| title_short | ADCK2 Knockdown Affects the Migration of Melanoma Cells via MYL6 |
| title_sort | adck2 knockdown affects the migration of melanoma cells via myl6 |
| topic | melanoma ADCK2 MYL6 motility cancer |
| url | https://www.mdpi.com/2072-6694/14/4/1071 |
| work_keys_str_mv | AT marlenevierthaler adck2knockdownaffectsthemigrationofmelanomacellsviamyl6 AT qiansun adck2knockdownaffectsthemigrationofmelanomacellsviamyl6 AT yimanwang adck2knockdownaffectsthemigrationofmelanomacellsviamyl6 AT tamarasteinfass adck2knockdownaffectsthemigrationofmelanomacellsviamyl6 AT julianepoelchen adck2knockdownaffectsthemigrationofmelanomacellsviamyl6 AT thomashielscher adck2knockdownaffectsthemigrationofmelanomacellsviamyl6 AT danielnovak adck2knockdownaffectsthemigrationofmelanomacellsviamyl6 AT viktorumansky adck2knockdownaffectsthemigrationofmelanomacellsviamyl6 AT jochenutikal adck2knockdownaffectsthemigrationofmelanomacellsviamyl6 |