A Novel Selenium Polysaccharide Alleviates the Manganese (Mn)-Induced Toxicity in Hep G2 Cells and <i>Caenorhabditis elegans</i>
Manganese (Mn) is now known to have a variety of toxicities, particularly when exposed to it in the workplace. However, there are still ineffective methods for reducing Mn’s hazardous effects. In this study, a new selenium polysaccharide (Se-PCS) was developed from the shell of <i>Camellia ole...
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/8/4097 |
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| author | Tao Chen Xiaoju Wang Xinchen Yan Yali Dai Tao Liang Lijun Zhou Shiling Feng Ming Yuan Hongyu Yang Chunbang Ding |
| author_facet | Tao Chen Xiaoju Wang Xinchen Yan Yali Dai Tao Liang Lijun Zhou Shiling Feng Ming Yuan Hongyu Yang Chunbang Ding |
| author_sort | Tao Chen |
| collection | DOAJ |
| description | Manganese (Mn) is now known to have a variety of toxicities, particularly when exposed to it in the workplace. However, there are still ineffective methods for reducing Mn’s hazardous effects. In this study, a new selenium polysaccharide (Se-PCS) was developed from the shell of <i>Camellia oleifera</i> to reduce Mn toxicity in vitro and in vivo. The results revealed that Se-PCS may boost cell survival in Hep G2 cells exposed to Mn and activate antioxidant enzyme activity, lowering ROS and cell apoptosis. Furthermore, after being treated with Se-PCS, <i>Caenorhabditis elegans</i> survived longer under Mn stress. <i>daf-16</i>, a tolerant critical gene, was turned on. Moreover, the antioxidant system was enhanced as the increase in strong antioxidant enzyme activity and high expression of the <i>sod-3</i>, <i>ctl-2</i>, and <i>gst-1</i> genes. A variety of mutations were also used to confirm that Se-PCS downregulated the insulin signaling pathway. These findings showed that Se-PCS protected Hep G2 cells and <i>C. elegans</i> via the insulin/IGF-1 signaling pathway and that it could be developed into a promising medication to treat Mn toxicity. |
| first_indexed | 2024-03-09T10:34:10Z |
| format | Article |
| id | doaj.art-729a34e7d78649e2bce9b88d40c8b684 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:34:10Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-729a34e7d78649e2bce9b88d40c8b6842023-12-01T21:01:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01238409710.3390/ijms23084097A Novel Selenium Polysaccharide Alleviates the Manganese (Mn)-Induced Toxicity in Hep G2 Cells and <i>Caenorhabditis elegans</i>Tao Chen0Xiaoju Wang1Xinchen Yan2Yali Dai3Tao Liang4Lijun Zhou5Shiling Feng6Ming Yuan7Hongyu Yang8Chunbang Ding9College of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaManganese (Mn) is now known to have a variety of toxicities, particularly when exposed to it in the workplace. However, there are still ineffective methods for reducing Mn’s hazardous effects. In this study, a new selenium polysaccharide (Se-PCS) was developed from the shell of <i>Camellia oleifera</i> to reduce Mn toxicity in vitro and in vivo. The results revealed that Se-PCS may boost cell survival in Hep G2 cells exposed to Mn and activate antioxidant enzyme activity, lowering ROS and cell apoptosis. Furthermore, after being treated with Se-PCS, <i>Caenorhabditis elegans</i> survived longer under Mn stress. <i>daf-16</i>, a tolerant critical gene, was turned on. Moreover, the antioxidant system was enhanced as the increase in strong antioxidant enzyme activity and high expression of the <i>sod-3</i>, <i>ctl-2</i>, and <i>gst-1</i> genes. A variety of mutations were also used to confirm that Se-PCS downregulated the insulin signaling pathway. These findings showed that Se-PCS protected Hep G2 cells and <i>C. elegans</i> via the insulin/IGF-1 signaling pathway and that it could be developed into a promising medication to treat Mn toxicity.https://www.mdpi.com/1422-0067/23/8/4097selenium polysaccharide<i>Caenorhabditis elegans</i><i>Camellia oleifera</i>manganese toxicityinsulin-like signaling pathway |
| spellingShingle | Tao Chen Xiaoju Wang Xinchen Yan Yali Dai Tao Liang Lijun Zhou Shiling Feng Ming Yuan Hongyu Yang Chunbang Ding A Novel Selenium Polysaccharide Alleviates the Manganese (Mn)-Induced Toxicity in Hep G2 Cells and <i>Caenorhabditis elegans</i> International Journal of Molecular Sciences selenium polysaccharide <i>Caenorhabditis elegans</i> <i>Camellia oleifera</i> manganese toxicity insulin-like signaling pathway |
| title | A Novel Selenium Polysaccharide Alleviates the Manganese (Mn)-Induced Toxicity in Hep G2 Cells and <i>Caenorhabditis elegans</i> |
| title_full | A Novel Selenium Polysaccharide Alleviates the Manganese (Mn)-Induced Toxicity in Hep G2 Cells and <i>Caenorhabditis elegans</i> |
| title_fullStr | A Novel Selenium Polysaccharide Alleviates the Manganese (Mn)-Induced Toxicity in Hep G2 Cells and <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | A Novel Selenium Polysaccharide Alleviates the Manganese (Mn)-Induced Toxicity in Hep G2 Cells and <i>Caenorhabditis elegans</i> |
| title_short | A Novel Selenium Polysaccharide Alleviates the Manganese (Mn)-Induced Toxicity in Hep G2 Cells and <i>Caenorhabditis elegans</i> |
| title_sort | novel selenium polysaccharide alleviates the manganese mn induced toxicity in hep g2 cells and i caenorhabditis elegans i |
| topic | selenium polysaccharide <i>Caenorhabditis elegans</i> <i>Camellia oleifera</i> manganese toxicity insulin-like signaling pathway |
| url | https://www.mdpi.com/1422-0067/23/8/4097 |
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