Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes model
<b>AIM:</b> To evaluate the influence of an intravitreal injection of bevacizumab and fasudil on the retinal vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNFα), and caspase 3 levels in a diabetic rabbit model.<b>RESULTS:</b> There was a statisticall...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2014-02-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949459/ |
| _version_ | 1819016677246894080 |
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| author | Fatih Çelik Fatih Ulaş Zeynep Güneş Özünal Tülin Fırat Serdal Çelebi Ümit Doğan |
| author_facet | Fatih Çelik Fatih Ulaş Zeynep Güneş Özünal Tülin Fırat Serdal Çelebi Ümit Doğan |
| author_sort | Fatih Çelik |
| collection | DOAJ |
| description | <b>AIM:</b> To evaluate the influence of an intravitreal injection of bevacizumab and fasudil on the retinal vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNFα), and caspase 3 levels in a diabetic rabbit model.<b>RESULTS:</b> There was a statistically significant difference in the VEGF and caspase 3 levels between groups (<i>P</i>=0.005 and <i>P</i> =0.013, respectively), but the TNFα level did not differ significantly between groups (<i>P</i>=0.792). It was found that VEGF levels were significantly lower in Group 1 and Group 3 than in Group 2 using the Mann-Whitney <i>U</i> test with the Bonferroni correction (<i>P</i>=0.004 for both comparison). There was no statistically significant difference between other groups with regard to VEGF levels (the <i>P</i> value ranged between 0.015 and 0.886). Although the <i>P</i> values of the caspase 3 levels were 0.015 for Group 1 and Group 4, 0.038 for Group 2 and Group 3, and 0.018 for Group 3 and Group 4, these <i>P</i> values remained above the threshold <i>P</i> value of 0.0083, which was the statistically significant level for post hoc tests.<b>CONCLUSION:</b> An intravitreal injection of bevacizumab decreased both the VEGF level, which plays a role in angiogenesis, and the caspase 3 level, which plays a role in apoptosis. Although not as effective as bevacizumab, fasudil had a beneficial effect on the VEGF levels but significantly increased the caspase 3 levels. |
| first_indexed | 2024-12-21T02:51:26Z |
| format | Article |
| id | doaj.art-729ad5673ef14e6689b54f0939373c7c |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-21T02:51:26Z |
| publishDate | 2014-02-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-729ad5673ef14e6689b54f0939373c7c2022-12-21T19:18:25ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982014-02-0171576110.3980/j.issn.2222-3959.2014.01.10Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes modelFatih Çelik0Fatih Ulaş1Zeynep Güneş Özünal2Tülin Fırat3Serdal Çelebi4Ümit Doğan5Department of Ophthalmology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey<br>Department of Ophthalmology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey<br>Department of Medical Pharmacology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey<br>Department of Histology and Embryology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, TurkeyDepartment of Ophthalmology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey<br>Department of Ophthalmology, Faculty of Medicine, Abant Izzet Baysal University, Bolu 14280, Turkey<br><b>AIM:</b> To evaluate the influence of an intravitreal injection of bevacizumab and fasudil on the retinal vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNFα), and caspase 3 levels in a diabetic rabbit model.<b>RESULTS:</b> There was a statistically significant difference in the VEGF and caspase 3 levels between groups (<i>P</i>=0.005 and <i>P</i> =0.013, respectively), but the TNFα level did not differ significantly between groups (<i>P</i>=0.792). It was found that VEGF levels were significantly lower in Group 1 and Group 3 than in Group 2 using the Mann-Whitney <i>U</i> test with the Bonferroni correction (<i>P</i>=0.004 for both comparison). There was no statistically significant difference between other groups with regard to VEGF levels (the <i>P</i> value ranged between 0.015 and 0.886). Although the <i>P</i> values of the caspase 3 levels were 0.015 for Group 1 and Group 4, 0.038 for Group 2 and Group 3, and 0.018 for Group 3 and Group 4, these <i>P</i> values remained above the threshold <i>P</i> value of 0.0083, which was the statistically significant level for post hoc tests.<b>CONCLUSION:</b> An intravitreal injection of bevacizumab decreased both the VEGF level, which plays a role in angiogenesis, and the caspase 3 level, which plays a role in apoptosis. Although not as effective as bevacizumab, fasudil had a beneficial effect on the VEGF levels but significantly increased the caspase 3 levels.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949459/bevacizumabcaspase 3experimental diabetesfasudiltumor necrosis factor alphavascular endothelial growth factorvascular endothelial growth factor |
| spellingShingle | Fatih Çelik Fatih Ulaş Zeynep Güneş Özünal Tülin Fırat Serdal Çelebi Ümit Doğan Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes model International Journal of Ophthalmology bevacizumab caspase 3 experimental diabetes fasudil tumor necrosis factor alpha vascular endothelial growth factor vascular endothelial growth factor |
| title | Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes model |
| title_full | Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes model |
| title_fullStr | Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes model |
| title_full_unstemmed | Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes model |
| title_short | Comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal VEGF, TNFα, and caspase 3 levels in an experimental diabetes model |
| title_sort | comparison of the effect of intravitreal bevacizumab and intravitreal fasudil on retinal vegf tnfα and caspase 3 levels in an experimental diabetes model |
| topic | bevacizumab caspase 3 experimental diabetes fasudil tumor necrosis factor alpha vascular endothelial growth factor vascular endothelial growth factor |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3949459/ |
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