Anti-Staphylococcal Activity of <i>Cinnamomum zeylanicum</i> Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological Infections
The aim of this study was to evaluate the phytochemical profile of <i>Cinnamomum zeylanicum</i> essential oil (CZEO) and their antimicrobial and antibiofilm activity against <i>Staphylococcus</i> strains isolated from canine otitis. First, the CZEO chemical composition was de...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/1/4 |
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| author | Vinicius de Queiroz Albuquerque Maria Janeila Carvalho Soares Maria Nágila Carneiro Matos Rafaela Mesquita Bastos Cavalcante Jesús Alberto Pérez Guerrero Tigressa Helena Soares Rodrigues Geovany Amorim Gomes Rodrigo Fonseca de Medeiros Guedes Débora de Souza Collares Maia Castelo-Branco Isaac Neto Goes da Silva Victor Alves Carneiro |
| author_facet | Vinicius de Queiroz Albuquerque Maria Janeila Carvalho Soares Maria Nágila Carneiro Matos Rafaela Mesquita Bastos Cavalcante Jesús Alberto Pérez Guerrero Tigressa Helena Soares Rodrigues Geovany Amorim Gomes Rodrigo Fonseca de Medeiros Guedes Débora de Souza Collares Maia Castelo-Branco Isaac Neto Goes da Silva Victor Alves Carneiro |
| author_sort | Vinicius de Queiroz Albuquerque |
| collection | DOAJ |
| description | The aim of this study was to evaluate the phytochemical profile of <i>Cinnamomum zeylanicum</i> essential oil (CZEO) and their antimicrobial and antibiofilm activity against <i>Staphylococcus</i> strains isolated from canine otitis. First, the CZEO chemical composition was determined by gas chromatography-mass spectrometry (CG-MS). External otitis samples collected from dogs were submitted to staphylococcal isolation, followed by MALDI-TOF mass spectrometry identification. The antimicrobial action was tested against the isolates using the disk-diffusion and microdilution methods. The antibiofilm activity was evaluated by CZEO-based concentrations, subMIC for biofilm formation and supraMIC against preformed biofilm, quantified by crystal violet (CV) staining and CFU counting. The chemical analysis revealed that (E)-cinnamaldehyde, eugenol and (E)-cinnamyl acetate were the main compounds in the CZEO, representing 77.42, 8.17 and 4.50%, respectively. Two strains of three different species, <i>S. saprophyticus</i>, <i>S. schleiferi</i> and <i>S. pseudintermedius</i>, were identified. The disk-diffusion test showed an inhibitory zone diameter, ranging from 34.0 to 49.5 mm, while the MIC and MBC values were around 500 and 1000 µg/mL. SubMIC demonstrated an inhibition on biofilm formation against 4 out the 6 strains tested. On mature biofilm, the CZEO-based supraMIC groups had slightly change on biomass, however, the biofilm cell viability decreased the CFU in 3 magnitude orders. |
| first_indexed | 2024-03-10T03:02:45Z |
| format | Article |
| id | doaj.art-729f3a719960432bbe78023ee33aed35 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:02:45Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-729f3a719960432bbe78023ee33aed352023-11-23T12:42:28ZengMDPI AGAntibiotics2079-63822021-12-01111410.3390/antibiotics11010004Anti-Staphylococcal Activity of <i>Cinnamomum zeylanicum</i> Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological InfectionsVinicius de Queiroz Albuquerque0Maria Janeila Carvalho Soares1Maria Nágila Carneiro Matos2Rafaela Mesquita Bastos Cavalcante3Jesús Alberto Pérez Guerrero4Tigressa Helena Soares Rodrigues5Geovany Amorim Gomes6Rodrigo Fonseca de Medeiros Guedes7Débora de Souza Collares Maia Castelo-Branco8Isaac Neto Goes da Silva9Victor Alves Carneiro10Department of Veterinary Sciences, State University of Ceará—Itaperi Campus, Fortaleza 60714-903, BrazilCenter for Bioprospecting and Applied Molecular Experimentation (NUBEM), University Center INTA-UNINTA, Sobral 62050-100, BrazilLaboratory of Biofilms and Antimicrobial Agents (LaBAM), Federal University of Ceará, Sobral 62048-280, BrazilLaboratory of Biofilms and Antimicrobial Agents (LaBAM), Federal University of Ceará, Sobral 62048-280, BrazilLaboratory of Biofilms and Antimicrobial Agents (LaBAM), Federal University of Ceará, Sobral 62048-280, BrazilCenter of Exact Science and Technology, State University of Acaraú Valley, Sobral 62040-370, BrazilCenter of Exact Science and Technology, State University of Acaraú Valley, Sobral 62040-370, BrazilGroup of Applied Medical Microbiology, Microbiology Department, Federal University of Ceará, Fortaleza 60430-160, BrazilGroup of Applied Medical Microbiology, Microbiology Department, Federal University of Ceará, Fortaleza 60430-160, BrazilDepartment of Veterinary Sciences, State University of Ceará—Itaperi Campus, Fortaleza 60714-903, BrazilCenter for Bioprospecting and Applied Molecular Experimentation (NUBEM), University Center INTA-UNINTA, Sobral 62050-100, BrazilThe aim of this study was to evaluate the phytochemical profile of <i>Cinnamomum zeylanicum</i> essential oil (CZEO) and their antimicrobial and antibiofilm activity against <i>Staphylococcus</i> strains isolated from canine otitis. First, the CZEO chemical composition was determined by gas chromatography-mass spectrometry (CG-MS). External otitis samples collected from dogs were submitted to staphylococcal isolation, followed by MALDI-TOF mass spectrometry identification. The antimicrobial action was tested against the isolates using the disk-diffusion and microdilution methods. The antibiofilm activity was evaluated by CZEO-based concentrations, subMIC for biofilm formation and supraMIC against preformed biofilm, quantified by crystal violet (CV) staining and CFU counting. The chemical analysis revealed that (E)-cinnamaldehyde, eugenol and (E)-cinnamyl acetate were the main compounds in the CZEO, representing 77.42, 8.17 and 4.50%, respectively. Two strains of three different species, <i>S. saprophyticus</i>, <i>S. schleiferi</i> and <i>S. pseudintermedius</i>, were identified. The disk-diffusion test showed an inhibitory zone diameter, ranging from 34.0 to 49.5 mm, while the MIC and MBC values were around 500 and 1000 µg/mL. SubMIC demonstrated an inhibition on biofilm formation against 4 out the 6 strains tested. On mature biofilm, the CZEO-based supraMIC groups had slightly change on biomass, however, the biofilm cell viability decreased the CFU in 3 magnitude orders.https://www.mdpi.com/2079-6382/11/1/4natural productantibiofilm activity<i>Staphylococcus</i> spp.canine-ear infections |
| spellingShingle | Vinicius de Queiroz Albuquerque Maria Janeila Carvalho Soares Maria Nágila Carneiro Matos Rafaela Mesquita Bastos Cavalcante Jesús Alberto Pérez Guerrero Tigressa Helena Soares Rodrigues Geovany Amorim Gomes Rodrigo Fonseca de Medeiros Guedes Débora de Souza Collares Maia Castelo-Branco Isaac Neto Goes da Silva Victor Alves Carneiro Anti-Staphylococcal Activity of <i>Cinnamomum zeylanicum</i> Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological Infections Antibiotics natural product antibiofilm activity <i>Staphylococcus</i> spp. canine-ear infections |
| title | Anti-Staphylococcal Activity of <i>Cinnamomum zeylanicum</i> Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological Infections |
| title_full | Anti-Staphylococcal Activity of <i>Cinnamomum zeylanicum</i> Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological Infections |
| title_fullStr | Anti-Staphylococcal Activity of <i>Cinnamomum zeylanicum</i> Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological Infections |
| title_full_unstemmed | Anti-Staphylococcal Activity of <i>Cinnamomum zeylanicum</i> Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological Infections |
| title_short | Anti-Staphylococcal Activity of <i>Cinnamomum zeylanicum</i> Essential Oil against Planktonic and Biofilm Cells Isolated from Canine Otological Infections |
| title_sort | anti staphylococcal activity of i cinnamomum zeylanicum i essential oil against planktonic and biofilm cells isolated from canine otological infections |
| topic | natural product antibiofilm activity <i>Staphylococcus</i> spp. canine-ear infections |
| url | https://www.mdpi.com/2079-6382/11/1/4 |
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