A Novel View of the Diversity of Anoxygenic Phototrophic Bacteria Inhabiting the Chemocline of Meromictic Karst Lakes
The rates of oxygenic and anoxygenic photosynthesis, the microorganisms responsible for these processes, and the hydrochemical characteristics of the sulfide-containing karst lakes, Black Kichier and Big Kichier (Mari El Republic), were investigated. In these lakes, a plate of anoxygenic phototrophi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/1/13 |
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| author | Vladimir Gorlenko Alexander Savvichev Vitaly Kadnikov Igor Rusanov Alexey Beletsky Elena Zakharova Nadezhda Kostrikina Pavel Sigalevich Elena Veslopolova Nikolay Pimenov |
| author_facet | Vladimir Gorlenko Alexander Savvichev Vitaly Kadnikov Igor Rusanov Alexey Beletsky Elena Zakharova Nadezhda Kostrikina Pavel Sigalevich Elena Veslopolova Nikolay Pimenov |
| author_sort | Vladimir Gorlenko |
| collection | DOAJ |
| description | The rates of oxygenic and anoxygenic photosynthesis, the microorganisms responsible for these processes, and the hydrochemical characteristics of the sulfide-containing karst lakes, Black Kichier and Big Kichier (Mari El Republic), were investigated. In these lakes, a plate of anoxygenic phototrophic bacteria (APB) is formed at the upper boundary of sulfide occurrence in the water. The phototrophic community of the chemocline zone was analyzed using a combination of high-throughput sequencing of the 16S rRNA gene fragments and light and electron microscopic techniques. Green-colored <i>Chlorobium clathratiforme</i> were absolutely predominant in both lakes. The minor components included green sulfur bacteria (GSB) <i>Chlorobium</i> spp., symbiotic consortia <i>Chlorochromatium magnum</i> and <i>Pelochromatium roseum</i>, purple sulfur bacteria (PSB) <i>Chromatium okenii</i>, and unidentified phylotypes of the family <i>Chromatiaceae</i>, as well as members of the <i>Chloroflexota</i>: <i>Chloronema</i> sp. and <i>Oscillochloris</i> sp. Based on the results of the molecular analysis, the taxonomic status of <i>Ancalochloris perfilievii</i> and other prosthecate GSB, as well as of the PSB <i>Thiopedia rosea</i>, which were visually revealed in the studied freshwater lakes, is discussed. |
| first_indexed | 2024-03-08T09:50:54Z |
| format | Article |
| id | doaj.art-72a269dfdd524c06bc3f148966dcf04f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T09:50:54Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-72a269dfdd524c06bc3f148966dcf04f2024-01-29T14:05:29ZengMDPI AGMicroorganisms2076-26072023-12-011211310.3390/microorganisms12010013A Novel View of the Diversity of Anoxygenic Phototrophic Bacteria Inhabiting the Chemocline of Meromictic Karst LakesVladimir Gorlenko0Alexander Savvichev1Vitaly Kadnikov2Igor Rusanov3Alexey Beletsky4Elena Zakharova5Nadezhda Kostrikina6Pavel Sigalevich7Elena Veslopolova8Nikolay Pimenov9Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaK.G. Skryabin Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaK.G. Skryabin Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaWinogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 117312 Moscow, RussiaThe rates of oxygenic and anoxygenic photosynthesis, the microorganisms responsible for these processes, and the hydrochemical characteristics of the sulfide-containing karst lakes, Black Kichier and Big Kichier (Mari El Republic), were investigated. In these lakes, a plate of anoxygenic phototrophic bacteria (APB) is formed at the upper boundary of sulfide occurrence in the water. The phototrophic community of the chemocline zone was analyzed using a combination of high-throughput sequencing of the 16S rRNA gene fragments and light and electron microscopic techniques. Green-colored <i>Chlorobium clathratiforme</i> were absolutely predominant in both lakes. The minor components included green sulfur bacteria (GSB) <i>Chlorobium</i> spp., symbiotic consortia <i>Chlorochromatium magnum</i> and <i>Pelochromatium roseum</i>, purple sulfur bacteria (PSB) <i>Chromatium okenii</i>, and unidentified phylotypes of the family <i>Chromatiaceae</i>, as well as members of the <i>Chloroflexota</i>: <i>Chloronema</i> sp. and <i>Oscillochloris</i> sp. Based on the results of the molecular analysis, the taxonomic status of <i>Ancalochloris perfilievii</i> and other prosthecate GSB, as well as of the PSB <i>Thiopedia rosea</i>, which were visually revealed in the studied freshwater lakes, is discussed.https://www.mdpi.com/2076-2607/12/1/13anoxygenic phototrophic bacteriameromictic karst lakeslake Kichiermicrobial communitybiodiversity<i>Chlorobium clathratiforme</i> |
| spellingShingle | Vladimir Gorlenko Alexander Savvichev Vitaly Kadnikov Igor Rusanov Alexey Beletsky Elena Zakharova Nadezhda Kostrikina Pavel Sigalevich Elena Veslopolova Nikolay Pimenov A Novel View of the Diversity of Anoxygenic Phototrophic Bacteria Inhabiting the Chemocline of Meromictic Karst Lakes Microorganisms anoxygenic phototrophic bacteria meromictic karst lakes lake Kichier microbial community biodiversity <i>Chlorobium clathratiforme</i> |
| title | A Novel View of the Diversity of Anoxygenic Phototrophic Bacteria Inhabiting the Chemocline of Meromictic Karst Lakes |
| title_full | A Novel View of the Diversity of Anoxygenic Phototrophic Bacteria Inhabiting the Chemocline of Meromictic Karst Lakes |
| title_fullStr | A Novel View of the Diversity of Anoxygenic Phototrophic Bacteria Inhabiting the Chemocline of Meromictic Karst Lakes |
| title_full_unstemmed | A Novel View of the Diversity of Anoxygenic Phototrophic Bacteria Inhabiting the Chemocline of Meromictic Karst Lakes |
| title_short | A Novel View of the Diversity of Anoxygenic Phototrophic Bacteria Inhabiting the Chemocline of Meromictic Karst Lakes |
| title_sort | novel view of the diversity of anoxygenic phototrophic bacteria inhabiting the chemocline of meromictic karst lakes |
| topic | anoxygenic phototrophic bacteria meromictic karst lakes lake Kichier microbial community biodiversity <i>Chlorobium clathratiforme</i> |
| url | https://www.mdpi.com/2076-2607/12/1/13 |
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