Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacum
Studies on the mechanics of plant cells usually focus on understanding the effects of turgor pressure and properties of the cell wall (CW). While the functional roles of the underlying cytoskeleton have been studied, the extent to which it contributes to the mechanical properties of cells is not elu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cambridge University Press
2022-01-01
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| Series: | Quantitative Plant Biology |
| Subjects: | |
| Online Access: | https://www.cambridge.org/core/product/identifier/S2632882821000151/type/journal_article |
| _version_ | 1811155739104772096 |
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| author | Leah Ginsberg Robin McDonald Qinchen Lin Rodinde Hendrickx Giada Spigolon Guruswami Ravichandran Chiara Daraio Eleftheria Roumeli |
| author_facet | Leah Ginsberg Robin McDonald Qinchen Lin Rodinde Hendrickx Giada Spigolon Guruswami Ravichandran Chiara Daraio Eleftheria Roumeli |
| author_sort | Leah Ginsberg |
| collection | DOAJ |
| description | Studies on the mechanics of plant cells usually focus on understanding the effects of turgor pressure and properties of the cell wall (CW). While the functional roles of the underlying cytoskeleton have been studied, the extent to which it contributes to the mechanical properties of cells is not elucidated. Here, we study the contributions of the CW, microtubules (MTs) and actin filaments (AFs), in the mechanical properties of Nicotiana tabacum cells. We use a multiscale biomechanical assay comprised of atomic force microscopy and micro-indentation in solutions that (i) remove MTs and AFs and (ii) alter osmotic pressures in the cells. To compare measurements obtained by the two mechanical tests, we develop two generative statistical models to describe the cell’s behaviour using one or both datasets. Our results illustrate that MTs and AFs contribute significantly to cell stiffness and dissipated energy, while confirming the dominant role of turgor pressure. |
| first_indexed | 2024-04-10T04:38:38Z |
| format | Article |
| id | doaj.art-72a90f31f33246c188a943ca1f92b05f |
| institution | Directory Open Access Journal |
| issn | 2632-8828 |
| language | English |
| last_indexed | 2024-04-10T04:38:38Z |
| publishDate | 2022-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Quantitative Plant Biology |
| spelling | doaj.art-72a90f31f33246c188a943ca1f92b05f2023-03-09T12:43:35ZengCambridge University PressQuantitative Plant Biology2632-88282022-01-01310.1017/qpb.2021.15Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacumLeah Ginsberg0Robin McDonald1Qinchen Lin2Rodinde Hendrickx3Giada Spigolon4Guruswami Ravichandran5Chiara Daraio6Eleftheria Roumeli7https://orcid.org/0000-0002-2828-1428Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USADivision of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USADepartment of Materials Science and Engineering, University of Washington, Seattle, WA 98195, USADivision of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USABiological Imaging Facility, California Institute of Technology, Pasadena, CA 91125, USADivision of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USADivision of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USADepartment of Materials Science and Engineering, University of Washington, Seattle, WA 98195, USAStudies on the mechanics of plant cells usually focus on understanding the effects of turgor pressure and properties of the cell wall (CW). While the functional roles of the underlying cytoskeleton have been studied, the extent to which it contributes to the mechanical properties of cells is not elucidated. Here, we study the contributions of the CW, microtubules (MTs) and actin filaments (AFs), in the mechanical properties of Nicotiana tabacum cells. We use a multiscale biomechanical assay comprised of atomic force microscopy and micro-indentation in solutions that (i) remove MTs and AFs and (ii) alter osmotic pressures in the cells. To compare measurements obtained by the two mechanical tests, we develop two generative statistical models to describe the cell’s behaviour using one or both datasets. Our results illustrate that MTs and AFs contribute significantly to cell stiffness and dissipated energy, while confirming the dominant role of turgor pressure.https://www.cambridge.org/core/product/identifier/S2632882821000151/type/journal_articlecell wallcytoskeletonmicro-indentationnano-indentation Nicotiana tabacum statistical modeling |
| spellingShingle | Leah Ginsberg Robin McDonald Qinchen Lin Rodinde Hendrickx Giada Spigolon Guruswami Ravichandran Chiara Daraio Eleftheria Roumeli Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacum Quantitative Plant Biology cell wall cytoskeleton micro-indentation nano-indentation Nicotiana tabacum statistical modeling |
| title | Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacum |
| title_full | Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacum |
| title_fullStr | Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacum |
| title_full_unstemmed | Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacum |
| title_short | Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacum |
| title_sort | cell wall and cytoskeletal contributions in single cell biomechanics of nicotiana tabacum |
| topic | cell wall cytoskeleton micro-indentation nano-indentation Nicotiana tabacum statistical modeling |
| url | https://www.cambridge.org/core/product/identifier/S2632882821000151/type/journal_article |
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