Morphological characterization and genetic analysis of Drechslera teres isolates
Net blotch, caused by the phytopathogen Drechslera teres, is a common disease of barley (Hordeum vulgare L) and is responsible for large economic losses in some barley growing areas. In this study the morphology and genetic variability of eight D. teres isolates from different regions of the Brazili...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2002-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200019 |
| _version_ | 1828779794139971584 |
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| author | A.P.G. Frazzon A.T.S. Matsumura S.T. Van Der Sand |
| author_facet | A.P.G. Frazzon A.T.S. Matsumura S.T. Van Der Sand |
| author_sort | A.P.G. Frazzon |
| collection | DOAJ |
| description | Net blotch, caused by the phytopathogen Drechslera teres, is a common disease of barley (Hordeum vulgare L) and is responsible for large economic losses in some barley growing areas. In this study the morphology and genetic variability of eight D. teres isolates from different regions of the Brazilian state of Rio Grande do Sul were investigated. Colony morphology was studied on potato-dextrose-agar (PDA) and genetic variability investigated using the random amplified polymorphic-DNA (RAPD) technique. 27 commercially available primers were tested of which 16 were selected for use in polymorphic analysis due to their good resolution and reproducibility. Similarity coefficients were used to construct dendrograms based on colony morphology and RAPD data showing the relationship between the eight isolates studied. Colony morphology showed variability between the isolates while RAPD assays showed high similarity coefficients, but grouping of the isolates according to the geographic origins of the seeds from which they were isolated was not possible. |
| first_indexed | 2024-12-11T17:07:30Z |
| format | Article |
| id | doaj.art-72aa16ec73704df2afa22550dbb7f105 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-11T17:07:30Z |
| publishDate | 2002-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-72aa16ec73704df2afa22550dbb7f1052022-12-22T00:57:38ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852002-01-0125223524110.1590/S1415-47572002000200019Morphological characterization and genetic analysis of Drechslera teres isolatesA.P.G. FrazzonA.T.S. MatsumuraS.T. Van Der SandNet blotch, caused by the phytopathogen Drechslera teres, is a common disease of barley (Hordeum vulgare L) and is responsible for large economic losses in some barley growing areas. In this study the morphology and genetic variability of eight D. teres isolates from different regions of the Brazilian state of Rio Grande do Sul were investigated. Colony morphology was studied on potato-dextrose-agar (PDA) and genetic variability investigated using the random amplified polymorphic-DNA (RAPD) technique. 27 commercially available primers were tested of which 16 were selected for use in polymorphic analysis due to their good resolution and reproducibility. Similarity coefficients were used to construct dendrograms based on colony morphology and RAPD data showing the relationship between the eight isolates studied. Colony morphology showed variability between the isolates while RAPD assays showed high similarity coefficients, but grouping of the isolates according to the geographic origins of the seeds from which they were isolated was not possible.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200019Drechslera teresbarleynet blotchDNA polymorphismRAPD |
| spellingShingle | A.P.G. Frazzon A.T.S. Matsumura S.T. Van Der Sand Morphological characterization and genetic analysis of Drechslera teres isolates Genetics and Molecular Biology Drechslera teres barley net blotch DNA polymorphism RAPD |
| title | Morphological characterization and genetic analysis of Drechslera teres isolates |
| title_full | Morphological characterization and genetic analysis of Drechslera teres isolates |
| title_fullStr | Morphological characterization and genetic analysis of Drechslera teres isolates |
| title_full_unstemmed | Morphological characterization and genetic analysis of Drechslera teres isolates |
| title_short | Morphological characterization and genetic analysis of Drechslera teres isolates |
| title_sort | morphological characterization and genetic analysis of drechslera teres isolates |
| topic | Drechslera teres barley net blotch DNA polymorphism RAPD |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200019 |
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