Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detection
The ongoing coronavirus pandemic responsible for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly increased the rate of global death and infections due to variant mutations (such as Delta and Omicron). While specifically developed and approved vaccines can limit the spread of...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-11-01
|
| Series: | Sensors and Actuators Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053922000510 |
| _version_ | 1811201791769968640 |
|---|---|
| author | Qingfeng Zhai Xichu Wang Chuangang Hu Lin Zhu Chenhao Zhang Liming Dai |
| author_facet | Qingfeng Zhai Xichu Wang Chuangang Hu Lin Zhu Chenhao Zhang Liming Dai |
| author_sort | Qingfeng Zhai |
| collection | DOAJ |
| description | The ongoing coronavirus pandemic responsible for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly increased the rate of global death and infections due to variant mutations (such as Delta and Omicron). While specifically developed and approved vaccines can limit the spread of disease in a population and severity of resulting symptoms, none have been demonstrated to effectively prevent infection altogether. Thus, reliable early diagnosis of COVID-19 is critical to identify positive cases to help contain the outbreak. Herein we report a label-free electrochemical immunosensor for rapid diagnosis of COVID-19 by using nitrogen-doped holey graphene (N-HRGO) as a nanocarrier decorated with thionine (TH) molecules as electrochemical indicators. With the spike protein located on the surface of the COVID-19 particles as the model target, the as-prepared electrochemical immunosensor could detect the presence of the COVID-19 spike protein over a wide linear range (1 pg mL−1−10 ng mL−1) with a low detection limit (0.3 pg mL−1). In addition, the developed electrochemical immunosensor exhibited an excellent selectivity (with insignificant current changes towards interfering proteins comparing with COVID-19 spike protein), a good reproducibility and long-term storage stability. Importantly, the electrochemical immunosensor thus developed could successfully and reliably detect the spike protein of COVID-19 in saliva and human serum complex samples. Thus, the as-prepared label-free electrochemical immunosensor can achieve rapid and sensitive detection of the COVID-19 spike protein, as a promising clinical diagnosis tool in monitoring the progression of COVID-19. |
| first_indexed | 2024-04-12T02:26:34Z |
| format | Article |
| id | doaj.art-72af000cd9be4d0ab51f524e4b584494 |
| institution | Directory Open Access Journal |
| issn | 2666-0539 |
| language | English |
| last_indexed | 2024-04-12T02:26:34Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj.art-72af000cd9be4d0ab51f524e4b5844942022-12-22T03:51:56ZengElsevierSensors and Actuators Reports2666-05392022-11-014100124Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detectionQingfeng Zhai0Xichu Wang1Chuangang Hu2Lin Zhu3Chenhao Zhang4Liming Dai5Australian Carbon Materials Centre (A-CMC), School of Chemical Engineering, University of New South Wales, Sydney, New South Wales 2052, AustraliaAustralian Carbon Materials Centre (A-CMC), School of Chemical Engineering, University of New South Wales, Sydney, New South Wales 2052, AustraliaAustralian Carbon Materials Centre (A-CMC), School of Chemical Engineering, University of New South Wales, Sydney, New South Wales 2052, AustraliaAustralian Carbon Materials Centre (A-CMC), School of Chemical Engineering, University of New South Wales, Sydney, New South Wales 2052, AustraliaAustralian Carbon Materials Centre (A-CMC), School of Chemical Engineering, University of New South Wales, Sydney, New South Wales 2052, AustraliaCorrespondence author.; Australian Carbon Materials Centre (A-CMC), School of Chemical Engineering, University of New South Wales, Sydney, New South Wales 2052, AustraliaThe ongoing coronavirus pandemic responsible for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly increased the rate of global death and infections due to variant mutations (such as Delta and Omicron). While specifically developed and approved vaccines can limit the spread of disease in a population and severity of resulting symptoms, none have been demonstrated to effectively prevent infection altogether. Thus, reliable early diagnosis of COVID-19 is critical to identify positive cases to help contain the outbreak. Herein we report a label-free electrochemical immunosensor for rapid diagnosis of COVID-19 by using nitrogen-doped holey graphene (N-HRGO) as a nanocarrier decorated with thionine (TH) molecules as electrochemical indicators. With the spike protein located on the surface of the COVID-19 particles as the model target, the as-prepared electrochemical immunosensor could detect the presence of the COVID-19 spike protein over a wide linear range (1 pg mL−1−10 ng mL−1) with a low detection limit (0.3 pg mL−1). In addition, the developed electrochemical immunosensor exhibited an excellent selectivity (with insignificant current changes towards interfering proteins comparing with COVID-19 spike protein), a good reproducibility and long-term storage stability. Importantly, the electrochemical immunosensor thus developed could successfully and reliably detect the spike protein of COVID-19 in saliva and human serum complex samples. Thus, the as-prepared label-free electrochemical immunosensor can achieve rapid and sensitive detection of the COVID-19 spike protein, as a promising clinical diagnosis tool in monitoring the progression of COVID-19.http://www.sciencedirect.com/science/article/pii/S2666053922000510Label-freeElectrochemical biosensorN-doped holey grapheneCovid-19 spike protein |
| spellingShingle | Qingfeng Zhai Xichu Wang Chuangang Hu Lin Zhu Chenhao Zhang Liming Dai Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detection Sensors and Actuators Reports Label-free Electrochemical biosensor N-doped holey graphene Covid-19 spike protein |
| title | Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detection |
| title_full | Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detection |
| title_fullStr | Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detection |
| title_full_unstemmed | Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detection |
| title_short | Label-free electrochemical immunosensor for highly sensitive COVID-19 spike protein detection |
| title_sort | label free electrochemical immunosensor for highly sensitive covid 19 spike protein detection |
| topic | Label-free Electrochemical biosensor N-doped holey graphene Covid-19 spike protein |
| url | http://www.sciencedirect.com/science/article/pii/S2666053922000510 |
| work_keys_str_mv | AT qingfengzhai labelfreeelectrochemicalimmunosensorforhighlysensitivecovid19spikeproteindetection AT xichuwang labelfreeelectrochemicalimmunosensorforhighlysensitivecovid19spikeproteindetection AT chuanganghu labelfreeelectrochemicalimmunosensorforhighlysensitivecovid19spikeproteindetection AT linzhu labelfreeelectrochemicalimmunosensorforhighlysensitivecovid19spikeproteindetection AT chenhaozhang labelfreeelectrochemicalimmunosensorforhighlysensitivecovid19spikeproteindetection AT limingdai labelfreeelectrochemicalimmunosensorforhighlysensitivecovid19spikeproteindetection |