Surfactin-Loaded ĸ-Carrageenan Oligosaccharides Entangled Cellulose Nanofibers as a Versatile Vehicle Against Periodontal Pathogens
Athira Johnson,1 Jia-Ling He,1 Fanbin Kong,2 Yi-Cheng Huang,1 Sabu Thomas,3 Hong-Ting Victor Lin,1 Zwe-Ling Kong1 1Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan; 2Department of Food Science and Technology, University of Georgia, GA 30602, U.S.A; 3School of Chemi...
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| Format: | Article |
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Dove Medical Press
2020-06-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/surfactin-loaded--carrageenan-oligosaccharides-entangled-cellulose-nan-peer-reviewed-article-IJN |
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| author | Johnson A He JL Kong F Huang YC Thomas S Lin HTV Kong ZL |
| author_facet | Johnson A He JL Kong F Huang YC Thomas S Lin HTV Kong ZL |
| author_sort | Johnson A |
| collection | DOAJ |
| description | Athira Johnson,1 Jia-Ling He,1 Fanbin Kong,2 Yi-Cheng Huang,1 Sabu Thomas,3 Hong-Ting Victor Lin,1 Zwe-Ling Kong1 1Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan; 2Department of Food Science and Technology, University of Georgia, GA 30602, U.S.A; 3School of Chemical Sciences, Mahatma Gandhi University, Kottayam, Kerala 686560, IndiaCorrespondence: Zwe-Ling KongDepartment of Food Science, National Taiwan Ocean University, Pei-Ning Road, Keelung City 20224, Taiwan, People’s Republic of ChinaTel + 886-2-2462-2192Fax + 886-2-2463-4203Email kongzl@mail.ntou.edu.twPurpose: Periodontitis is a chronic inflammatory disease associated with microbial accumulation. The purpose of this study was to reuse the agricultural waste to produce cellulose nanofibers (CNF) and further modification of the CNF with κ-carrageenan oligosaccharides (CO) for drug delivery. In addition, this study is focused on the antimicrobial activity of surfactin-loaded CO-CNF towards periodontal pathogens.Materials and Methods: A chemo-mechanical method was used to extract the CNF and the modification was done by using CO. The studies were further proceeded by adding different quantities of surfactin [50 mg (50 SNPs), 100 mg (100 SNPs), 200 mg (200 SNPs)] into the carrier (CO-CNF). The obtained materials were characterized, and the antimicrobial activity of surfactin-loaded CO-CNF was evaluated.Results: The obtained average size of CNF and CO-CNF after ultrasonication was 263 nm and 330 nm, respectively. Microscopic studies suggested that the CNF has a short diameter with long length and CO became cross-linked to form as beads within the CNF network. The addition of CO improved the degradation temperature, crystallinity, and swelling property of CNF. The material has a controlled drug release, and the entrapment efficiency and loading capacity of the drug were 53.15 ± 2.36% and 36.72 ± 1.24%, respectively. It has antioxidant activity and inhibited the growth of periodontal pathogens such as Streptococcus mutans and Porphyromonas gingivalis by preventing the biofilm formation, reducing the metabolic activity, and promoting the oxidative stress.Conclusion: The study showed the successful extraction of CNF and modification with CO improved the physical parameters of the CNF. In addition, surfactin-loaded CO-CNF has potential antimicrobial activity against periodontal pathogens. The obtained biomaterial is economically valuable and has great potential for biomedical applications.Keywords: periodontitis, cellulose nanofibers, κ-carrageenan oligosaccharides, drug delivery, antimicrobial activity |
| first_indexed | 2024-12-17T10:26:56Z |
| format | Article |
| id | doaj.art-72afe05677b54b4d9857eae44ca2c8c7 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-17T10:26:56Z |
| publishDate | 2020-06-01 |
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| series | International Journal of Nanomedicine |
| spelling | doaj.art-72afe05677b54b4d9857eae44ca2c8c72022-12-21T21:52:38ZengDove Medical PressInternational Journal of Nanomedicine1178-20132020-06-01Volume 154021404754381Surfactin-Loaded ĸ-Carrageenan Oligosaccharides Entangled Cellulose Nanofibers as a Versatile Vehicle Against Periodontal PathogensJohnson AHe JLKong FHuang YCThomas SLin HTVKong ZLAthira Johnson,1 Jia-Ling He,1 Fanbin Kong,2 Yi-Cheng Huang,1 Sabu Thomas,3 Hong-Ting Victor Lin,1 Zwe-Ling Kong1 1Department of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan; 2Department of Food Science and Technology, University of Georgia, GA 30602, U.S.A; 3School of Chemical Sciences, Mahatma Gandhi University, Kottayam, Kerala 686560, IndiaCorrespondence: Zwe-Ling KongDepartment of Food Science, National Taiwan Ocean University, Pei-Ning Road, Keelung City 20224, Taiwan, People’s Republic of ChinaTel + 886-2-2462-2192Fax + 886-2-2463-4203Email kongzl@mail.ntou.edu.twPurpose: Periodontitis is a chronic inflammatory disease associated with microbial accumulation. The purpose of this study was to reuse the agricultural waste to produce cellulose nanofibers (CNF) and further modification of the CNF with κ-carrageenan oligosaccharides (CO) for drug delivery. In addition, this study is focused on the antimicrobial activity of surfactin-loaded CO-CNF towards periodontal pathogens.Materials and Methods: A chemo-mechanical method was used to extract the CNF and the modification was done by using CO. The studies were further proceeded by adding different quantities of surfactin [50 mg (50 SNPs), 100 mg (100 SNPs), 200 mg (200 SNPs)] into the carrier (CO-CNF). The obtained materials were characterized, and the antimicrobial activity of surfactin-loaded CO-CNF was evaluated.Results: The obtained average size of CNF and CO-CNF after ultrasonication was 263 nm and 330 nm, respectively. Microscopic studies suggested that the CNF has a short diameter with long length and CO became cross-linked to form as beads within the CNF network. The addition of CO improved the degradation temperature, crystallinity, and swelling property of CNF. The material has a controlled drug release, and the entrapment efficiency and loading capacity of the drug were 53.15 ± 2.36% and 36.72 ± 1.24%, respectively. It has antioxidant activity and inhibited the growth of periodontal pathogens such as Streptococcus mutans and Porphyromonas gingivalis by preventing the biofilm formation, reducing the metabolic activity, and promoting the oxidative stress.Conclusion: The study showed the successful extraction of CNF and modification with CO improved the physical parameters of the CNF. In addition, surfactin-loaded CO-CNF has potential antimicrobial activity against periodontal pathogens. The obtained biomaterial is economically valuable and has great potential for biomedical applications.Keywords: periodontitis, cellulose nanofibers, κ-carrageenan oligosaccharides, drug delivery, antimicrobial activityhttps://www.dovepress.com/surfactin-loaded--carrageenan-oligosaccharides-entangled-cellulose-nan-peer-reviewed-article-IJNperiodontitiscellulose nanofibersκ-carrageenan oligosaccharidesdrug deliveryantimicrobial activity |
| spellingShingle | Johnson A He JL Kong F Huang YC Thomas S Lin HTV Kong ZL Surfactin-Loaded ĸ-Carrageenan Oligosaccharides Entangled Cellulose Nanofibers as a Versatile Vehicle Against Periodontal Pathogens International Journal of Nanomedicine periodontitis cellulose nanofibers κ-carrageenan oligosaccharides drug delivery antimicrobial activity |
| title | Surfactin-Loaded ĸ-Carrageenan Oligosaccharides Entangled Cellulose Nanofibers as a Versatile Vehicle Against Periodontal Pathogens |
| title_full | Surfactin-Loaded ĸ-Carrageenan Oligosaccharides Entangled Cellulose Nanofibers as a Versatile Vehicle Against Periodontal Pathogens |
| title_fullStr | Surfactin-Loaded ĸ-Carrageenan Oligosaccharides Entangled Cellulose Nanofibers as a Versatile Vehicle Against Periodontal Pathogens |
| title_full_unstemmed | Surfactin-Loaded ĸ-Carrageenan Oligosaccharides Entangled Cellulose Nanofibers as a Versatile Vehicle Against Periodontal Pathogens |
| title_short | Surfactin-Loaded ĸ-Carrageenan Oligosaccharides Entangled Cellulose Nanofibers as a Versatile Vehicle Against Periodontal Pathogens |
| title_sort | surfactin loaded q carrageenan oligosaccharides entangled cellulose nanofibers as a versatile vehicle against periodontal pathogens |
| topic | periodontitis cellulose nanofibers κ-carrageenan oligosaccharides drug delivery antimicrobial activity |
| url | https://www.dovepress.com/surfactin-loaded--carrageenan-oligosaccharides-entangled-cellulose-nan-peer-reviewed-article-IJN |
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