EGb 761 reduces Ca2+ influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell line
BackgroundTransient receptor potential (TRP) channels have been found to have significant implications in neuronal outgrowth, survival, inflammatory neurogenic pain, and various epileptogenic processes. Moreover, there is a growing body of evidence indicating that transient receptor potential (TRP)...
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| Format: | Article |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2023.1195303/full |
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| author | Ishak Suat Ovey Ahmet Ozsimsek Halil Aziz Velioglu Halil Aziz Velioglu Ozlem Altay Adil Mardinoglu Burak Yulug Burak Yulug |
| author_facet | Ishak Suat Ovey Ahmet Ozsimsek Halil Aziz Velioglu Halil Aziz Velioglu Ozlem Altay Adil Mardinoglu Burak Yulug Burak Yulug |
| author_sort | Ishak Suat Ovey |
| collection | DOAJ |
| description | BackgroundTransient receptor potential (TRP) channels have been found to have significant implications in neuronal outgrowth, survival, inflammatory neurogenic pain, and various epileptogenic processes. Moreover, there is a growing body of evidence indicating that transient receptor potential (TRP) channels have a significant impact on epilepsy and its drug-resistant subtypes.ObjectiveWe postulated that EGb 761 would modulate TRPA1 channels, thereby exhibiting anti-inflammatory and neuroprotective effects in a neuroblastoma cell line. Our rationale was to investigate the impact of EGb 761 in a controlled model of pentylenetetrazole-induced generalized epilepsy.MethodologyWe evaluated the neuroprotective, antioxidant and anti-apoptotic effects of EGb 761 both before and after the pentylenetetrazole application in a neuroblastoma cell line. Specifically, we focused on the effects of EGB 761 on the activity of Transient receptor potential (TRP) channels.ResultsEGb 761 applications both before and after the pentylenetetrazole incubation period reduced Ca release and restored apoptosis, ROS changes, mitochondrial depolarization and caspase levels, suggesting a prominent prophylactic and therapeutic effect of EGb 761 in the pentylenetetrazole-induced epileptogenesis process.ConclusionOur basic mechanistic framework for elucidating the pathophysiological significance of fundamental ion mechanisms in a pentylenetetrazole treated neuroblastoma cell line provided compelling evidence for the favorable efficacy and safety profile of Egb 761 in human-relevant in vitro model of epilepsy. To the best of our knowledge, this is the first study to investigate the combined effects of EGb 761 and pentylenetetrazole on TRP channels and measure their activation level in a relevant model of human epileptic diseases. |
| first_indexed | 2024-03-12T02:02:12Z |
| format | Article |
| id | doaj.art-72b0694c9619448a9577e32081f88c51 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-03-12T02:02:12Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-72b0694c9619448a9577e32081f88c512023-09-07T11:09:26ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022023-09-011710.3389/fncel.2023.11953031195303EGb 761 reduces Ca2+ influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell lineIshak Suat Ovey0Ahmet Ozsimsek1Halil Aziz Velioglu2Halil Aziz Velioglu3Ozlem Altay4Adil Mardinoglu5Burak Yulug6Burak Yulug7Department of Physiology, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya, TürkiyeDepartment of Neurology and Neuroscience, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya, TürkiyeDepartment of Neuroscience, Faculty of Medicine, Istanbul Medipol University, Istanbul, TürkiyeCenter for Psychiatric Neuroscience, Feinstein Institutes for Medical Research, Manhasset, NY, United StatesKTH Royal Institute of Technology, Stockholm, SwedenKTH Royal Institute of Technology, Stockholm, SwedenDepartment of Neurology and Neuroscience, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya, TürkiyeDepartment of Neuroscience, Faculty of Medicine, Istanbul Medipol University, Istanbul, TürkiyeBackgroundTransient receptor potential (TRP) channels have been found to have significant implications in neuronal outgrowth, survival, inflammatory neurogenic pain, and various epileptogenic processes. Moreover, there is a growing body of evidence indicating that transient receptor potential (TRP) channels have a significant impact on epilepsy and its drug-resistant subtypes.ObjectiveWe postulated that EGb 761 would modulate TRPA1 channels, thereby exhibiting anti-inflammatory and neuroprotective effects in a neuroblastoma cell line. Our rationale was to investigate the impact of EGb 761 in a controlled model of pentylenetetrazole-induced generalized epilepsy.MethodologyWe evaluated the neuroprotective, antioxidant and anti-apoptotic effects of EGb 761 both before and after the pentylenetetrazole application in a neuroblastoma cell line. Specifically, we focused on the effects of EGB 761 on the activity of Transient receptor potential (TRP) channels.ResultsEGb 761 applications both before and after the pentylenetetrazole incubation period reduced Ca release and restored apoptosis, ROS changes, mitochondrial depolarization and caspase levels, suggesting a prominent prophylactic and therapeutic effect of EGb 761 in the pentylenetetrazole-induced epileptogenesis process.ConclusionOur basic mechanistic framework for elucidating the pathophysiological significance of fundamental ion mechanisms in a pentylenetetrazole treated neuroblastoma cell line provided compelling evidence for the favorable efficacy and safety profile of Egb 761 in human-relevant in vitro model of epilepsy. To the best of our knowledge, this is the first study to investigate the combined effects of EGb 761 and pentylenetetrazole on TRP channels and measure their activation level in a relevant model of human epileptic diseases.https://www.frontiersin.org/articles/10.3389/fncel.2023.1195303/fulltransient receptor potential (TRP) channelsEGb 761epilepsy EGbneuroprotecciónginseng |
| spellingShingle | Ishak Suat Ovey Ahmet Ozsimsek Halil Aziz Velioglu Halil Aziz Velioglu Ozlem Altay Adil Mardinoglu Burak Yulug Burak Yulug EGb 761 reduces Ca2+ influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell line Frontiers in Cellular Neuroscience transient receptor potential (TRP) channels EGb 761 epilepsy EGb neuroprotección ginseng |
| title | EGb 761 reduces Ca2+ influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell line |
| title_full | EGb 761 reduces Ca2+ influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell line |
| title_fullStr | EGb 761 reduces Ca2+ influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell line |
| title_full_unstemmed | EGb 761 reduces Ca2+ influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell line |
| title_short | EGb 761 reduces Ca2+ influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell line |
| title_sort | egb 761 reduces ca2 influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell line |
| topic | transient receptor potential (TRP) channels EGb 761 epilepsy EGb neuroprotección ginseng |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2023.1195303/full |
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