In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila
Summary: NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein in vivo, without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in Drosophila, starting from a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006682 |
| _version_ | 1811183862415360000 |
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| author | Jocelyn L.Y. Tang Robert Krautz Oriol Llorà-Batlle Anna E. Hakes Paul M. Fox Andrea H. Brand |
| author_facet | Jocelyn L.Y. Tang Robert Krautz Oriol Llorà-Batlle Anna E. Hakes Paul M. Fox Andrea H. Brand |
| author_sort | Jocelyn L.Y. Tang |
| collection | DOAJ |
| description | Summary: NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein in vivo, without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in Drosophila, starting from a genetic cross, to the generation of sequencing libraries and, finally, bioinformatic analysis. This protocol can be readily adapted for use in other model systems after simple modifications.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T13:03:46Z |
| format | Article |
| id | doaj.art-72b0855f41e54215b2bc2a9c33b41fcb |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T13:03:46Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-72b0855f41e54215b2bc2a9c33b41fcb2022-12-22T04:22:50ZengElsevierSTAR Protocols2666-16672022-12-0134101788In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in DrosophilaJocelyn L.Y. Tang0Robert Krautz1Oriol Llorà-Batlle2Anna E. Hakes3Paul M. Fox4Andrea H. Brand5Gurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UKGurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UKGurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UKGurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UKGurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UKGurdon Institute and Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK; Corresponding authorSummary: NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein in vivo, without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in Drosophila, starting from a genetic cross, to the generation of sequencing libraries and, finally, bioinformatic analysis. This protocol can be readily adapted for use in other model systems after simple modifications.For complete details on the use and execution of this protocol, please refer to Tang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006682Cell biologyDevelopmental biologyGenomicsSequencingModel organisms |
| spellingShingle | Jocelyn L.Y. Tang Robert Krautz Oriol Llorà-Batlle Anna E. Hakes Paul M. Fox Andrea H. Brand In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila STAR Protocols Cell biology Developmental biology Genomics Sequencing Model organisms |
| title | In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila |
| title_full | In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila |
| title_fullStr | In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila |
| title_full_unstemmed | In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila |
| title_short | In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila |
| title_sort | in vivo genome wide profiling of endogenously tagged chromatin binding proteins with spatial and temporal resolution using nanodam in drosophila |
| topic | Cell biology Developmental biology Genomics Sequencing Model organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006682 |
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