In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila

In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila

Summary: NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein in vivo, without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in Drosophila, starting from a...

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Bibliographic Details
Main Authors: Jocelyn L.Y. Tang, Robert Krautz, Oriol Llorà-Batlle, Anna E. Hakes, Paul M. Fox, Andrea H. Brand
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell biology
Developmental biology
Genomics
Sequencing
Model organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006682

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