One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography
A method for batch preparation of fucoxanthin from brown algae was established, which possessed the advantages of high yield and high purity. The ultrasonic-assisted extraction method was used to obtain a crude extract from <i>Sargassum fusiforme</i> as the separation sample. Then the cr...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/20/4/257 |
| _version_ | 1797434425471926272 |
|---|---|
| author | Danting Chen Yating Jin Di Hu Jing Ye Yanbin Lu Zhiyuan Dai |
| author_facet | Danting Chen Yating Jin Di Hu Jing Ye Yanbin Lu Zhiyuan Dai |
| author_sort | Danting Chen |
| collection | DOAJ |
| description | A method for batch preparation of fucoxanthin from brown algae was established, which possessed the advantages of high yield and high purity. The ultrasonic-assisted extraction method was used to obtain a crude extract from <i>Sargassum fusiforme</i> as the separation sample. Then the crude extract was separated by elution-extrusion countercurrent chromatography. The optimum preparation conditions of fucoxanthin were determined as follows: <i>n</i>-hexane-ethanol-water (20:9:11, <i>v</i>:<i>v</i>:<i>v</i>) as a two-phase solvent system, the mobile phase flow rate was 5 mL min<sup>−1</sup>, the revolution speed was 800 r min<sup>−1</sup>, the loading capacity was 60 mg 10 mL<sup>−1</sup> and the temperature was 25 °C. By this method, 12.8 mg fucoxanthin with a purity of 94.72% was obtained from the crude extract of <i>Sargassum fusiforme</i>. In addition, when the loading capacity was 50 mg 10 mL<sup>−1</sup>, the purity of fucoxanthin reached 96.01%. Two types of by-products, chlorophyll and pheophytin, could also be obtained during the process of separation. This optimal method was further applied to separate fucoxanthin from <i>Laminaria japonica</i> and <i>Undaria pinnatifida</i>, and 6.0 mg and 9.7 mg fucoxanthin with a purity of 96.24% and 92.62% were acquired, respectively. Therefore, it was demonstrated that the preparation method of fucoxanthin established in this study had an applicability to brown algae, which improved the utilization value of raw materials. |
| first_indexed | 2024-03-09T10:32:03Z |
| format | Article |
| id | doaj.art-72b32afc08c843858a920413ccc1fc92 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T10:32:03Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-72b32afc08c843858a920413ccc1fc922023-12-01T21:10:58ZengMDPI AGMarine Drugs1660-33972022-04-0120425710.3390/md20040257One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent ChromatographyDanting Chen0Yating Jin1Di Hu2Jing Ye3Yanbin Lu4Zhiyuan Dai5Key Laboratory of Aquatic Products Processing of Zhejiang Province, Collaborative Innovation Center of Seafood Deep Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, ChinaKey Laboratory of Aquatic Products Processing of Zhejiang Province, Collaborative Innovation Center of Seafood Deep Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, ChinaKey Laboratory of Aquatic Products Processing of Zhejiang Province, Collaborative Innovation Center of Seafood Deep Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, ChinaKey Laboratory of Aquatic Products Processing of Zhejiang Province, Collaborative Innovation Center of Seafood Deep Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, ChinaKey Laboratory of Aquatic Products Processing of Zhejiang Province, Collaborative Innovation Center of Seafood Deep Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, ChinaKey Laboratory of Aquatic Products Processing of Zhejiang Province, Collaborative Innovation Center of Seafood Deep Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, ChinaA method for batch preparation of fucoxanthin from brown algae was established, which possessed the advantages of high yield and high purity. The ultrasonic-assisted extraction method was used to obtain a crude extract from <i>Sargassum fusiforme</i> as the separation sample. Then the crude extract was separated by elution-extrusion countercurrent chromatography. The optimum preparation conditions of fucoxanthin were determined as follows: <i>n</i>-hexane-ethanol-water (20:9:11, <i>v</i>:<i>v</i>:<i>v</i>) as a two-phase solvent system, the mobile phase flow rate was 5 mL min<sup>−1</sup>, the revolution speed was 800 r min<sup>−1</sup>, the loading capacity was 60 mg 10 mL<sup>−1</sup> and the temperature was 25 °C. By this method, 12.8 mg fucoxanthin with a purity of 94.72% was obtained from the crude extract of <i>Sargassum fusiforme</i>. In addition, when the loading capacity was 50 mg 10 mL<sup>−1</sup>, the purity of fucoxanthin reached 96.01%. Two types of by-products, chlorophyll and pheophytin, could also be obtained during the process of separation. This optimal method was further applied to separate fucoxanthin from <i>Laminaria japonica</i> and <i>Undaria pinnatifida</i>, and 6.0 mg and 9.7 mg fucoxanthin with a purity of 96.24% and 92.62% were acquired, respectively. Therefore, it was demonstrated that the preparation method of fucoxanthin established in this study had an applicability to brown algae, which improved the utilization value of raw materials.https://www.mdpi.com/1660-3397/20/4/257brown algae<i>Sargassum fusiforme</i><i>Laminaria japonica</i><i>Undaria pinnatifida</i>fucoxanthinseparation |
| spellingShingle | Danting Chen Yating Jin Di Hu Jing Ye Yanbin Lu Zhiyuan Dai One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography Marine Drugs brown algae <i>Sargassum fusiforme</i> <i>Laminaria japonica</i> <i>Undaria pinnatifida</i> fucoxanthin separation |
| title | One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography |
| title_full | One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography |
| title_fullStr | One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography |
| title_full_unstemmed | One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography |
| title_short | One-Step Preparative Separation of Fucoxanthin from Three Edible Brown Algae by Elution-Extrusion Countercurrent Chromatography |
| title_sort | one step preparative separation of fucoxanthin from three edible brown algae by elution extrusion countercurrent chromatography |
| topic | brown algae <i>Sargassum fusiforme</i> <i>Laminaria japonica</i> <i>Undaria pinnatifida</i> fucoxanthin separation |
| url | https://www.mdpi.com/1660-3397/20/4/257 |
| work_keys_str_mv | AT dantingchen onesteppreparativeseparationoffucoxanthinfromthreeediblebrownalgaebyelutionextrusioncountercurrentchromatography AT yatingjin onesteppreparativeseparationoffucoxanthinfromthreeediblebrownalgaebyelutionextrusioncountercurrentchromatography AT dihu onesteppreparativeseparationoffucoxanthinfromthreeediblebrownalgaebyelutionextrusioncountercurrentchromatography AT jingye onesteppreparativeseparationoffucoxanthinfromthreeediblebrownalgaebyelutionextrusioncountercurrentchromatography AT yanbinlu onesteppreparativeseparationoffucoxanthinfromthreeediblebrownalgaebyelutionextrusioncountercurrentchromatography AT zhiyuandai onesteppreparativeseparationoffucoxanthinfromthreeediblebrownalgaebyelutionextrusioncountercurrentchromatography |