| Summary: | This work reports the synthesis and application of uniform and spherical molecularly imprinted polymeric (MIP) nanobeads for selective and efficient preconcentration of Rosmarinic acid (RA). The as-prepared RA MIP nanobeads were characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and Brunauer–Emmett–Teller (BET) techniques. As analyzed, nano-sized particles of MIP with spherical and uniform shape and the average size of 88 nm were synthesized. The prepared MIP nanobeads were used in an ultrasonic-assisted dispersive solid phase extraction approach (UA-DSPE) for extraction of RA as a natural antioxidant specie from the extract of Salvia Officinalis leaves. In UA-DSPE process, the RA content of the samples was determined using high-performance liquid chromatography (HPLC). The highest recovery percentage (>90%) was observed after loading of RA solution (pH 5.7) and after elution with 3 mL of acetic acid aqueous solution, followed by 6 min sonication. The selectivity studies demonstrated a selective and high affinity binding of the template molecule (i.e. RA) toward MIP sorbent compared to other compounds such as gallic acid, caffeic acid, and pyrocatechol.
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