An Off-the-Shelf Approach for the Production of Fc Fusion Proteins by Protein <i>Trans</i>-Splicing towards Generating a Lectibody In Vitro
Monoclonal antibodies, engineered antibodies, and antibody fragments have become important biological therapeutic platforms. The IgG format with bivalent binding sites has a modular structure with different biological roles, i.e., effector and binding functions, in different domains. We demonstrated...
Main Authors: | Anniina Jaakkonen, Gerrit Volkmann, Hideo Iwaï |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-06-01
|
Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/11/4011 |
Similar Items
-
The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms
by: Hannes M. Beyer, et al.
Published: (2020-11-01) -
Inteins—mechanism of protein splicing, emerging regulatory roles, and applications in protein engineering
by: David W. Wood, et al.
Published: (2023-11-01) -
Semi-Biosynthetic Production of Surface-Binding Adhesive Antimicrobial Peptides Using Intein-Mediated Protein Ligation
by: Young Eun Hwang, et al.
Published: (2022-12-01) -
The Inducible Intein-Mediated Self-Cleaving Tag (IIST) System: A Novel Purification and Amidation System for Peptides and Proteins
by: A. Sesilja Aranko, et al.
Published: (2021-09-01) -
Mini-Intein Structures from Extremophiles Suggest a Strategy for Finding Novel Robust Inteins
by: Mimmu K. Hiltunen, et al.
Published: (2021-06-01)