A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses
Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) are four identified porcine enteric coronaviruses. Pigs infected with these viruses show similar manifestations of diarrhea,...
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MDPI AG
2022-11-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/11/634 |
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| author | Hongjin Zhou Kaichuang Shi Feng Long Kang Zhao Shuping Feng Yanwen Yin Chenyong Xiong Sujie Qu Wenjun Lu Zongqiang Li |
| author_facet | Hongjin Zhou Kaichuang Shi Feng Long Kang Zhao Shuping Feng Yanwen Yin Chenyong Xiong Sujie Qu Wenjun Lu Zongqiang Li |
| author_sort | Hongjin Zhou |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) are four identified porcine enteric coronaviruses. Pigs infected with these viruses show similar manifestations of diarrhea, vomiting, and dehydration. Here, a quadruplex real-time quantitative PCR (qRT-PCR) assay was established for the differential detection of PEDV, TGEV, PDCoV, and SADS-CoV from swine fecal samples. The assay showed extreme specificity, high sensitivity, and excellent reproducibility, with the limit of detection (LOD) of 121 copies/μL (final reaction concentration of 12.1 copies/μL) for each virus. The 3236 clinical fecal samples from Guangxi province in China collected between October 2020 and October 2022 were evaluated by the quadruplex qRT-PCR, and the positive rates of PEDV, TGEV, PDCoV, and SADS-CoV were 18.26% (591/3236), 0.46% (15/3236), 13.16% (426/3236), and 0.15% (5/3236), respectively. The samples were also evaluated by the multiplex qRT-PCR reported previously by other scientists, and the compliance rate between the two methods was more than 99%. This illustrated that the developed quadruplex qRT-PCR assay can provide an accurate method for the differential detection of four porcine enteric coronaviruses. |
| first_indexed | 2024-03-09T17:56:15Z |
| format | Article |
| id | doaj.art-72ba8212846a46ac97c1f6a24effbf75 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T17:56:15Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-72ba8212846a46ac97c1f6a24effbf752023-11-24T10:17:45ZengMDPI AGVeterinary Sciences2306-73812022-11-0191163410.3390/vetsci9110634A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric CoronavirusesHongjin Zhou0Kaichuang Shi1Feng Long2Kang Zhao3Shuping Feng4Yanwen Yin5Chenyong Xiong6Sujie Qu7Wenjun Lu8Zongqiang Li9College of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, ChinaGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, ChinaGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530005, ChinaPorcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) are four identified porcine enteric coronaviruses. Pigs infected with these viruses show similar manifestations of diarrhea, vomiting, and dehydration. Here, a quadruplex real-time quantitative PCR (qRT-PCR) assay was established for the differential detection of PEDV, TGEV, PDCoV, and SADS-CoV from swine fecal samples. The assay showed extreme specificity, high sensitivity, and excellent reproducibility, with the limit of detection (LOD) of 121 copies/μL (final reaction concentration of 12.1 copies/μL) for each virus. The 3236 clinical fecal samples from Guangxi province in China collected between October 2020 and October 2022 were evaluated by the quadruplex qRT-PCR, and the positive rates of PEDV, TGEV, PDCoV, and SADS-CoV were 18.26% (591/3236), 0.46% (15/3236), 13.16% (426/3236), and 0.15% (5/3236), respectively. The samples were also evaluated by the multiplex qRT-PCR reported previously by other scientists, and the compliance rate between the two methods was more than 99%. This illustrated that the developed quadruplex qRT-PCR assay can provide an accurate method for the differential detection of four porcine enteric coronaviruses.https://www.mdpi.com/2306-7381/9/11/634porcine enteric coronavirusporcine epidemic diarrhea virus (PEDV)transmissible gastroenteritis virus (TGEV)porcine deltacoronavirus (PDCoV)swine acute diarrhea syndrome coronavirus (SADS-CoV)multiplex qRT-PCR |
| spellingShingle | Hongjin Zhou Kaichuang Shi Feng Long Kang Zhao Shuping Feng Yanwen Yin Chenyong Xiong Sujie Qu Wenjun Lu Zongqiang Li A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses Veterinary Sciences porcine enteric coronavirus porcine epidemic diarrhea virus (PEDV) transmissible gastroenteritis virus (TGEV) porcine deltacoronavirus (PDCoV) swine acute diarrhea syndrome coronavirus (SADS-CoV) multiplex qRT-PCR |
| title | A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses |
| title_full | A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses |
| title_fullStr | A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses |
| title_full_unstemmed | A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses |
| title_short | A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses |
| title_sort | quadruplex qrt pcr for differential detection of four porcine enteric coronaviruses |
| topic | porcine enteric coronavirus porcine epidemic diarrhea virus (PEDV) transmissible gastroenteritis virus (TGEV) porcine deltacoronavirus (PDCoV) swine acute diarrhea syndrome coronavirus (SADS-CoV) multiplex qRT-PCR |
| url | https://www.mdpi.com/2306-7381/9/11/634 |
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