Forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammation
Abstract Background Inflammation and biofilm formation by Staphylococcus aureus (S. aureus) are common causes of periprosthetic infection and loosening. Recently, we identified that forsythiaside is bacteriostatic for S. aureus and methicillin-resistant S. aureus (MRSA). The purpose of the present s...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-06-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-018-0834-x |
| _version_ | 1811301186935980032 |
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| author | Haifeng Li Dongmei Tang Chao Qi Xia Zhao Guangchao Wang Yi Zhang Tengbo Yu |
| author_facet | Haifeng Li Dongmei Tang Chao Qi Xia Zhao Guangchao Wang Yi Zhang Tengbo Yu |
| author_sort | Haifeng Li |
| collection | DOAJ |
| description | Abstract Background Inflammation and biofilm formation by Staphylococcus aureus (S. aureus) are common causes of periprosthetic infection and loosening. Recently, we identified that forsythiaside is bacteriostatic for S. aureus and methicillin-resistant S. aureus (MRSA). The purpose of the present study was to examine the effect of forsythiaside on S. aureus and MRSA adhesion and biofilm formation on the surface of titanium alloy, which is a popular material for orthopedic joint prostheses. Methods Two strains of S. aureus and MRSA were used for in vitro experiments. The spread plate method, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) were used to characterize antimicrobial activity of forsythiaside. Real-time polymerase chain reaction (RT-PCR) and western blotting were used to investigate the inhibitory level of forsythiaside required for titanium-associated inflammation. Results Direct colony counting showed that 16 μg/mL forsythiaside significantly inhibited S. aureus and MRSA adhesion on titanium alloy discs in 2 h. CLSM and SEM showed that higher concentrations (> 30 mg/mL) of forsythiaside effectively inhibited the adhesion of S. aureus and MRSA on the surface of the titanium disc in 24 h. Forsythiaside was capable of attenuating Ti-induced activation of nuclear factor-κB signaling, targeting IκB kinase-α (IKKα) kinases of macrophages, and influencing the expression of NF-κB downstream cytokines. Conclusions These observations suggest that forsythiaside is a potential agent for the treatment of Ti implant-associated infection and inflammation. |
| first_indexed | 2024-04-13T07:04:24Z |
| format | Article |
| id | doaj.art-72bac1702f0d4bffb2a414305e210063 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-13T07:04:24Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-72bac1702f0d4bffb2a414305e2100632022-12-22T02:57:02ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2018-06-0113111010.1186/s13018-018-0834-xForsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammationHaifeng Li0Dongmei Tang1Chao Qi2Xia Zhao3Guangchao Wang4Yi Zhang5Tengbo Yu6Department of Sports Medicine, The Affiliated Hospital of Qingdao UniversityDepartment of Anesthesiology, The Affiliated Hospital of Qingdao UniversityDepartment of Sports Medicine, The Affiliated Hospital of Qingdao UniversityDepartment of Sports Medicine, The Affiliated Hospital of Qingdao UniversityDepartment of Orthopedics, Changhai Hospital, the Second Military Medical UniversityDepartment of Sports Medicine, The Affiliated Hospital of Qingdao UniversityDepartment of Sports Medicine, The Affiliated Hospital of Qingdao UniversityAbstract Background Inflammation and biofilm formation by Staphylococcus aureus (S. aureus) are common causes of periprosthetic infection and loosening. Recently, we identified that forsythiaside is bacteriostatic for S. aureus and methicillin-resistant S. aureus (MRSA). The purpose of the present study was to examine the effect of forsythiaside on S. aureus and MRSA adhesion and biofilm formation on the surface of titanium alloy, which is a popular material for orthopedic joint prostheses. Methods Two strains of S. aureus and MRSA were used for in vitro experiments. The spread plate method, confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) were used to characterize antimicrobial activity of forsythiaside. Real-time polymerase chain reaction (RT-PCR) and western blotting were used to investigate the inhibitory level of forsythiaside required for titanium-associated inflammation. Results Direct colony counting showed that 16 μg/mL forsythiaside significantly inhibited S. aureus and MRSA adhesion on titanium alloy discs in 2 h. CLSM and SEM showed that higher concentrations (> 30 mg/mL) of forsythiaside effectively inhibited the adhesion of S. aureus and MRSA on the surface of the titanium disc in 24 h. Forsythiaside was capable of attenuating Ti-induced activation of nuclear factor-κB signaling, targeting IκB kinase-α (IKKα) kinases of macrophages, and influencing the expression of NF-κB downstream cytokines. Conclusions These observations suggest that forsythiaside is a potential agent for the treatment of Ti implant-associated infection and inflammation.http://link.springer.com/article/10.1186/s13018-018-0834-xTitaniumForsythiasideAnti-bacteriaAnti-inflammationNuclear factor-κB signaling pathway |
| spellingShingle | Haifeng Li Dongmei Tang Chao Qi Xia Zhao Guangchao Wang Yi Zhang Tengbo Yu Forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammation Journal of Orthopaedic Surgery and Research Titanium Forsythiaside Anti-bacteria Anti-inflammation Nuclear factor-κB signaling pathway |
| title | Forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammation |
| title_full | Forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammation |
| title_fullStr | Forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammation |
| title_full_unstemmed | Forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammation |
| title_short | Forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates Ti-induced activation of nuclear factor-κB signaling-mediated macrophage inflammation |
| title_sort | forsythiaside inhibits bacterial adhesion on titanium alloy and attenuates ti induced activation of nuclear factor κb signaling mediated macrophage inflammation |
| topic | Titanium Forsythiaside Anti-bacteria Anti-inflammation Nuclear factor-κB signaling pathway |
| url | http://link.springer.com/article/10.1186/s13018-018-0834-x |
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