Insights into the impacts of autotoxic allelochemicals from rhizosphere of Atractylodes lancea on soil microenvironments
Atractylodes lancea suffers from continuous cropping obstacles that have become a major constraint in its cultivation, but there is still little information on the autotoxic allelochemicals and their interaction with soil microorganisms. In this study, we firstly identified the autotoxic allelochemi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1136833/full |
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| author | Meng Wang Juan Deng Gonghao Duan Lei Chen Xiao Huang Xiao Huang Wenjie Wang Ling Gong Yan Zhang Kun Yu Kun Yu Lanping Guo |
| author_facet | Meng Wang Juan Deng Gonghao Duan Lei Chen Xiao Huang Xiao Huang Wenjie Wang Ling Gong Yan Zhang Kun Yu Kun Yu Lanping Guo |
| author_sort | Meng Wang |
| collection | DOAJ |
| description | Atractylodes lancea suffers from continuous cropping obstacles that have become a major constraint in its cultivation, but there is still little information on the autotoxic allelochemicals and their interaction with soil microorganisms. In this study, we firstly identified the autotoxic allelochemicals from rhizosphere of A. lancea and determined their autotoxicity. Third-year continuous A. lancea cropping soils, i.e., rhizospheric soil and bulk soil, compared with control soil and one-year natural fallow soil were used to determine soil biochemical properties and microbial community. Eight allelochemicals from A. lancea roots were detected and exhibited significant autotoxicity effects on seed germination and seedling growth of A. lancea with the highest content of dibutyl phthalate in rhizospheric soil and lowest IC50 value of 2,4-di-tert-butylphenol inhibiting seed germination. The contents of soil nutrients and organic matter, pH value, and enzyme activity were altered between different soils, and the parameters of fallow soil were close to those of the unplanted soil. The PCoA analysis indicated that the community composition of both bacteria and fungi were differed significantly among the soil samples. Continuous cropping decreased OTUs numbers of bacterial and fungal communities, and natural fallow restored them. The relative abundance of Proteobacteria, Planctomycetes, and Actinobacteria decreased, and that of Acidobacteria and Ascomycota increased after three years cultivation. The LEfSe analysis identified 115 and 49 biomarkers for bacterial and fungal communities, respectively. The results suggested that natural fallow restored the structure of soil microbial community. Overall, our results revealed that autotoxic allelochemicals caused the variations of soil microenvironments and resulted in replantation problem of A. lancea, and natural fallow alleviated the soil deterioration by remodeling the rhizospheric microbial community and restoring soil biochemical properties. These findings provide important insights and clues for solving the continuous cropping problems and guiding the management of sustainable farmland. |
| first_indexed | 2024-04-10T04:33:55Z |
| format | Article |
| id | doaj.art-72bca93685fc4f63a9a4a4d373cf1b7e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T04:33:55Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-72bca93685fc4f63a9a4a4d373cf1b7e2023-03-10T04:42:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-03-011410.3389/fpls.2023.11368331136833Insights into the impacts of autotoxic allelochemicals from rhizosphere of Atractylodes lancea on soil microenvironmentsMeng Wang0Juan Deng1Gonghao Duan2Lei Chen3Xiao Huang4Xiao Huang5Wenjie Wang6Ling Gong7Yan Zhang8Kun Yu9Kun Yu10Lanping Guo11College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaHubei Key Laboratory of Resources and Chemistry of Chinese Medicine, Hubei University of Chinese Medicine, Wuhan, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaCollege of Pharmacy, Hubei University of Chinese Medicine, Wuhan, ChinaHubei Key Laboratory of Resources and Chemistry of Chinese Medicine, Hubei University of Chinese Medicine, Wuhan, ChinaState Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, ChinaAtractylodes lancea suffers from continuous cropping obstacles that have become a major constraint in its cultivation, but there is still little information on the autotoxic allelochemicals and their interaction with soil microorganisms. In this study, we firstly identified the autotoxic allelochemicals from rhizosphere of A. lancea and determined their autotoxicity. Third-year continuous A. lancea cropping soils, i.e., rhizospheric soil and bulk soil, compared with control soil and one-year natural fallow soil were used to determine soil biochemical properties and microbial community. Eight allelochemicals from A. lancea roots were detected and exhibited significant autotoxicity effects on seed germination and seedling growth of A. lancea with the highest content of dibutyl phthalate in rhizospheric soil and lowest IC50 value of 2,4-di-tert-butylphenol inhibiting seed germination. The contents of soil nutrients and organic matter, pH value, and enzyme activity were altered between different soils, and the parameters of fallow soil were close to those of the unplanted soil. The PCoA analysis indicated that the community composition of both bacteria and fungi were differed significantly among the soil samples. Continuous cropping decreased OTUs numbers of bacterial and fungal communities, and natural fallow restored them. The relative abundance of Proteobacteria, Planctomycetes, and Actinobacteria decreased, and that of Acidobacteria and Ascomycota increased after three years cultivation. The LEfSe analysis identified 115 and 49 biomarkers for bacterial and fungal communities, respectively. The results suggested that natural fallow restored the structure of soil microbial community. Overall, our results revealed that autotoxic allelochemicals caused the variations of soil microenvironments and resulted in replantation problem of A. lancea, and natural fallow alleviated the soil deterioration by remodeling the rhizospheric microbial community and restoring soil biochemical properties. These findings provide important insights and clues for solving the continuous cropping problems and guiding the management of sustainable farmland.https://www.frontiersin.org/articles/10.3389/fpls.2023.1136833/fullallelochemicalsAtractylodes lanceamicrobial communitynatural fallowroot exudates |
| spellingShingle | Meng Wang Juan Deng Gonghao Duan Lei Chen Xiao Huang Xiao Huang Wenjie Wang Ling Gong Yan Zhang Kun Yu Kun Yu Lanping Guo Insights into the impacts of autotoxic allelochemicals from rhizosphere of Atractylodes lancea on soil microenvironments Frontiers in Plant Science allelochemicals Atractylodes lancea microbial community natural fallow root exudates |
| title | Insights into the impacts of autotoxic allelochemicals from rhizosphere of Atractylodes lancea on soil microenvironments |
| title_full | Insights into the impacts of autotoxic allelochemicals from rhizosphere of Atractylodes lancea on soil microenvironments |
| title_fullStr | Insights into the impacts of autotoxic allelochemicals from rhizosphere of Atractylodes lancea on soil microenvironments |
| title_full_unstemmed | Insights into the impacts of autotoxic allelochemicals from rhizosphere of Atractylodes lancea on soil microenvironments |
| title_short | Insights into the impacts of autotoxic allelochemicals from rhizosphere of Atractylodes lancea on soil microenvironments |
| title_sort | insights into the impacts of autotoxic allelochemicals from rhizosphere of atractylodes lancea on soil microenvironments |
| topic | allelochemicals Atractylodes lancea microbial community natural fallow root exudates |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1136833/full |
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