Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice
The severity of pneumonia in respiratory syncytial virus (RSV) infection is strongly related to host immune response and external factors such as bacteria and environmental chemicals. We investigated the effect of inactivated Streptococcus pneumoniae (ISP) as non-pathogenic particles on the severity...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-01-01
|
| Series: | Toxicology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750018306966 |
| _version_ | 1818036738729181184 |
|---|---|
| author | Aki Miyauchi Wataru Watanabe Toshi Akashi Seiko Hashiguchi Hiroki Yoshida Chihiro Sugita Masahiko Kurokawa |
| author_facet | Aki Miyauchi Wataru Watanabe Toshi Akashi Seiko Hashiguchi Hiroki Yoshida Chihiro Sugita Masahiko Kurokawa |
| author_sort | Aki Miyauchi |
| collection | DOAJ |
| description | The severity of pneumonia in respiratory syncytial virus (RSV) infection is strongly related to host immune response and external factors such as bacteria and environmental chemicals. We investigated the effect of inactivated Streptococcus pneumoniae (ISP) as non-pathogenic particles on the severity of pneumonia in RSV-infected mice. Mice were intranasally exposed to ISP before RSV infection. On day 5 post-infection, we examined tissues, virus titer, and infiltrated cells in the lungs. The ISP did not cause significant histopathological effects in the lungs of RSV infected mice, but reduced virus titer. It also reduced the ratio of lymphocyte infiltration into the lungs and consequently the ratio of macrophage increased. In addition, we found that ISP increased RANTES level in bronchoalveolar lavage fluid from RSV-infected mice on day 1 post-infection, but reduced type I interferon levels. Thus, ISP did not exacerbate pneumonia in RSV infection, rather, it might mildly reduce the severity. We characterize and discuss the inherent activity of ISP as non-pathogenic particles inducing the role of RANTES on the pneumonia in RSV infection. Keywords: RSV, Streptococcus pneumonia, Pneumonia, Infiltrated cells, Non-pathogenic pneumococcal particles |
| first_indexed | 2024-12-10T07:15:43Z |
| format | Article |
| id | doaj.art-72c5d23aee1542f083faeab18ffb1fb2 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-10T07:15:43Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-72c5d23aee1542f083faeab18ffb1fb22022-12-22T01:57:56ZengElsevierToxicology Reports2214-75002019-01-016514520Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in miceAki Miyauchi0Wataru Watanabe1Toshi Akashi2Seiko Hashiguchi3Hiroki Yoshida4Chihiro Sugita5Masahiko Kurokawa6Departments of Biochemistry and Microbiology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, JapanDepartments of Biochemistry and Microbiology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, JapanDepartment of Microbiology and Infectious Diseases, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, JapanDepartments of Biochemistry and Microbiology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, JapanDepartments of Biochemistry and Microbiology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, JapanDepartments of Biochemistry and Microbiology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, JapanDepartments of Biochemistry and Microbiology, Graduate School of Clinical Pharmacy, Kyushu University of Health and Welfare, 1714-1 Yoshino, Nobeoka, Miyazaki 882-8508, Japan; Corresponding author.The severity of pneumonia in respiratory syncytial virus (RSV) infection is strongly related to host immune response and external factors such as bacteria and environmental chemicals. We investigated the effect of inactivated Streptococcus pneumoniae (ISP) as non-pathogenic particles on the severity of pneumonia in RSV-infected mice. Mice were intranasally exposed to ISP before RSV infection. On day 5 post-infection, we examined tissues, virus titer, and infiltrated cells in the lungs. The ISP did not cause significant histopathological effects in the lungs of RSV infected mice, but reduced virus titer. It also reduced the ratio of lymphocyte infiltration into the lungs and consequently the ratio of macrophage increased. In addition, we found that ISP increased RANTES level in bronchoalveolar lavage fluid from RSV-infected mice on day 1 post-infection, but reduced type I interferon levels. Thus, ISP did not exacerbate pneumonia in RSV infection, rather, it might mildly reduce the severity. We characterize and discuss the inherent activity of ISP as non-pathogenic particles inducing the role of RANTES on the pneumonia in RSV infection. Keywords: RSV, Streptococcus pneumonia, Pneumonia, Infiltrated cells, Non-pathogenic pneumococcal particleshttp://www.sciencedirect.com/science/article/pii/S2214750018306966 |
| spellingShingle | Aki Miyauchi Wataru Watanabe Toshi Akashi Seiko Hashiguchi Hiroki Yoshida Chihiro Sugita Masahiko Kurokawa Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice Toxicology Reports |
| title | Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice |
| title_full | Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice |
| title_fullStr | Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice |
| title_full_unstemmed | Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice |
| title_short | Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice |
| title_sort | effect of inactivated streptococcus pneumoniae as non pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice |
| url | http://www.sciencedirect.com/science/article/pii/S2214750018306966 |
| work_keys_str_mv | AT akimiyauchi effectofinactivatedstreptococcuspneumoniaeasnonpathogenicparticlesontheseverityofpneumoniacausedbyrespiratorysyncytialvirusinfectioninmice AT wataruwatanabe effectofinactivatedstreptococcuspneumoniaeasnonpathogenicparticlesontheseverityofpneumoniacausedbyrespiratorysyncytialvirusinfectioninmice AT toshiakashi effectofinactivatedstreptococcuspneumoniaeasnonpathogenicparticlesontheseverityofpneumoniacausedbyrespiratorysyncytialvirusinfectioninmice AT seikohashiguchi effectofinactivatedstreptococcuspneumoniaeasnonpathogenicparticlesontheseverityofpneumoniacausedbyrespiratorysyncytialvirusinfectioninmice AT hirokiyoshida effectofinactivatedstreptococcuspneumoniaeasnonpathogenicparticlesontheseverityofpneumoniacausedbyrespiratorysyncytialvirusinfectioninmice AT chihirosugita effectofinactivatedstreptococcuspneumoniaeasnonpathogenicparticlesontheseverityofpneumoniacausedbyrespiratorysyncytialvirusinfectioninmice AT masahikokurokawa effectofinactivatedstreptococcuspneumoniaeasnonpathogenicparticlesontheseverityofpneumoniacausedbyrespiratorysyncytialvirusinfectioninmice |