In vitro production of cattle blastocysts in chemically defined medium with or without insulin supplementation
In this study we evaluated the use of a chemically defined medium in the production of blastocysts from bovine oocytes fertilized in vitro. As culture medium we used CRI-PVP, a modification of CRlaa medium with bovine serum albumin replaced by polyvinylpyrrolidone. After 168 h of culture (192 h afte...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Scientific Agricultural Society of Finland
1996-09-01
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| Series: | Agricultural and Food Science |
| Online Access: | https://journal.fi/afs/article/view/72762 |
| _version_ | 1811300194737717248 |
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| author | Kristiina Bredbacka Peter Bredbacka |
| author_facet | Kristiina Bredbacka Peter Bredbacka |
| author_sort | Kristiina Bredbacka |
| collection | DOAJ |
| description | In this study we evaluated the use of a chemically defined medium in the production of blastocysts from bovine oocytes fertilized in vitro. As culture medium we used CRI-PVP, a modification of CRlaa medium with bovine serum albumin replaced by polyvinylpyrrolidone. After 168 h of culture (192 h after insemination) 8.7%, 10.5 and 12.8% of the cleaved embryos developed to the blastocyst stage in the presence of 0, 2 or 200 nM insulin, respectively. The supplementation of 200 nM insulin tended to increase cell numbers in morulae and blastocysts (P=0.10). It is concluded that CRI-PVP can be used as a chemically defined medium in the production of blastocysts from bovine 1-cell embryos. However, further modifications are needed, and the insulin concentrations used may be below the optimum for blastocyst production. |
| first_indexed | 2024-04-13T06:47:40Z |
| format | Article |
| id | doaj.art-72c61f843b8e4da6887ab543a8e35c63 |
| institution | Directory Open Access Journal |
| issn | 1459-6067 1795-1895 |
| language | English |
| last_indexed | 2024-04-13T06:47:40Z |
| publishDate | 1996-09-01 |
| publisher | Scientific Agricultural Society of Finland |
| record_format | Article |
| series | Agricultural and Food Science |
| spelling | doaj.art-72c61f843b8e4da6887ab543a8e35c632022-12-22T02:57:31ZengScientific Agricultural Society of FinlandAgricultural and Food Science1459-60671795-18951996-09-0155In vitro production of cattle blastocysts in chemically defined medium with or without insulin supplementationKristiina Bredbacka0Peter Bredbacka1Agricultural Research Centre of Finland, Institute of Animal Production, FIN-31600 Jokioinen, FinlandAgricultural Research Centre of Finland, Institute of Animal Production, FIN-31600, Jokioinen, FinlandIn this study we evaluated the use of a chemically defined medium in the production of blastocysts from bovine oocytes fertilized in vitro. As culture medium we used CRI-PVP, a modification of CRlaa medium with bovine serum albumin replaced by polyvinylpyrrolidone. After 168 h of culture (192 h after insemination) 8.7%, 10.5 and 12.8% of the cleaved embryos developed to the blastocyst stage in the presence of 0, 2 or 200 nM insulin, respectively. The supplementation of 200 nM insulin tended to increase cell numbers in morulae and blastocysts (P=0.10). It is concluded that CRI-PVP can be used as a chemically defined medium in the production of blastocysts from bovine 1-cell embryos. However, further modifications are needed, and the insulin concentrations used may be below the optimum for blastocyst production.https://journal.fi/afs/article/view/72762 |
| spellingShingle | Kristiina Bredbacka Peter Bredbacka In vitro production of cattle blastocysts in chemically defined medium with or without insulin supplementation Agricultural and Food Science |
| title | In vitro production of cattle blastocysts in chemically defined medium with or without insulin supplementation |
| title_full | In vitro production of cattle blastocysts in chemically defined medium with or without insulin supplementation |
| title_fullStr | In vitro production of cattle blastocysts in chemically defined medium with or without insulin supplementation |
| title_full_unstemmed | In vitro production of cattle blastocysts in chemically defined medium with or without insulin supplementation |
| title_short | In vitro production of cattle blastocysts in chemically defined medium with or without insulin supplementation |
| title_sort | in vitro production of cattle blastocysts in chemically defined medium with or without insulin supplementation |
| url | https://journal.fi/afs/article/view/72762 |
| work_keys_str_mv | AT kristiinabredbacka invitroproductionofcattleblastocystsinchemicallydefinedmediumwithorwithoutinsulinsupplementation AT peterbredbacka invitroproductionofcattleblastocystsinchemicallydefinedmediumwithorwithoutinsulinsupplementation |