Effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggs
We explored the effects of different conditions on the artificial incubation of redclaw crayfish eggs in an effort to improve this process. Samples at the egg and juvenile stages were selected. The samples at different stages were separated from the pleopods, then they were placed in incubator boxes...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023080039 |
| _version_ | 1797429985941651456 |
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| author | Shun Cheng Man Liu Yong-chun Wei Mei-li Chi Jian-bo Zheng Wen-ping Jiang Shi-li Liu Xiao-ying Hang Miao Peng Fei Li Dan-li Wang |
| author_facet | Shun Cheng Man Liu Yong-chun Wei Mei-li Chi Jian-bo Zheng Wen-ping Jiang Shi-li Liu Xiao-ying Hang Miao Peng Fei Li Dan-li Wang |
| author_sort | Shun Cheng |
| collection | DOAJ |
| description | We explored the effects of different conditions on the artificial incubation of redclaw crayfish eggs in an effort to improve this process. Samples at the egg and juvenile stages were selected. The samples at different stages were separated from the pleopods, then they were placed in incubator boxes and sterilized with different disinfectant solutions. The density was 300,400 and 500 eggs/incubator box, the vibration frequency was 11,16 and 26 vibrations/min, and the water circulation cycle was 2.1, 4.8 and 7.1 cycles/h. The results showed the eggs disinfected with 3000 ppm formaldehyde for 15 min had stronger antioxidant capacity. The hatching and survival rates of five pairs of appendage stage group were significantly lower than those of other groups. In the egg stage, acid phosphatase (ACP) level of compound eye pigmentation stage group was significantly higher than those of other groups. In the juvenile stage, malondialdehyde (MDA) content of five pairs of appendage stage group was significantly higher than those of other groups. The survival rate of 500 eggs/box group was significantly higher than that of other groups. In the egg stage, alkaline phosphatase (AKP) level of 400 eggs/box group was significantly higher than that of other groups. The survival rate of 11 vibrations/min group was significantly higher than that of other groups. In the egg stage, ACP and AKP levels of 11 vibrations/min group were significantly higher than those of 26 vibrations/min group. In the juvenile stage, superoxide dismutase (SOD), ACP and AKP levels of 11 vibrations/min group was significantly higher than those of 26 vibrations/min group. In the juvenile stage, AKP level of 4.8 cycles/h group was significantly lower than that of other groups. In conclusion, egg development at the stage after seven pairs of appendages, with a density of 400 eggs/box, vibration frequencies set at 11 vibrations/min achieved high hatching rates (93.58 %) and survival rates (75.67 %). Moreover, bronopol or hydrogen peroxide might have a better choice to replace formaldehyde if further exploration was conducted to reduce stimulation of the in vitro-grown egg. These conditions could be used on a large scale to optimize the production of redclaw crayfish. |
| first_indexed | 2024-03-09T09:21:11Z |
| format | Article |
| id | doaj.art-72c7752e73b14b6a8b52ecf6cffaf843 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:21:11Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-72c7752e73b14b6a8b52ecf6cffaf8432023-12-02T07:00:54ZengElsevierHeliyon2405-84402023-11-01911e20795Effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggsShun Cheng0Man Liu1Yong-chun Wei2Mei-li Chi3Jian-bo Zheng4Wen-ping Jiang5Shi-li Liu6Xiao-ying Hang7Miao Peng8Fei Li9Dan-li Wang10Zhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China; School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, ChinaFood and Drug Inspection and Testing Center of Deqing, Huzhou Zhejiang 313200 ChinaZhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, ChinaZhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, ChinaZhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, ChinaZhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, ChinaZhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, ChinaZhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, ChinaZhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, ChinaZhejiang Institute of Freshwater Fisheries, Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture / Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Huzhou Zhejiang 313001, China; Corresponding author.School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, China; Corresponding author.We explored the effects of different conditions on the artificial incubation of redclaw crayfish eggs in an effort to improve this process. Samples at the egg and juvenile stages were selected. The samples at different stages were separated from the pleopods, then they were placed in incubator boxes and sterilized with different disinfectant solutions. The density was 300,400 and 500 eggs/incubator box, the vibration frequency was 11,16 and 26 vibrations/min, and the water circulation cycle was 2.1, 4.8 and 7.1 cycles/h. The results showed the eggs disinfected with 3000 ppm formaldehyde for 15 min had stronger antioxidant capacity. The hatching and survival rates of five pairs of appendage stage group were significantly lower than those of other groups. In the egg stage, acid phosphatase (ACP) level of compound eye pigmentation stage group was significantly higher than those of other groups. In the juvenile stage, malondialdehyde (MDA) content of five pairs of appendage stage group was significantly higher than those of other groups. The survival rate of 500 eggs/box group was significantly higher than that of other groups. In the egg stage, alkaline phosphatase (AKP) level of 400 eggs/box group was significantly higher than that of other groups. The survival rate of 11 vibrations/min group was significantly higher than that of other groups. In the egg stage, ACP and AKP levels of 11 vibrations/min group were significantly higher than those of 26 vibrations/min group. In the juvenile stage, superoxide dismutase (SOD), ACP and AKP levels of 11 vibrations/min group was significantly higher than those of 26 vibrations/min group. In the juvenile stage, AKP level of 4.8 cycles/h group was significantly lower than that of other groups. In conclusion, egg development at the stage after seven pairs of appendages, with a density of 400 eggs/box, vibration frequencies set at 11 vibrations/min achieved high hatching rates (93.58 %) and survival rates (75.67 %). Moreover, bronopol or hydrogen peroxide might have a better choice to replace formaldehyde if further exploration was conducted to reduce stimulation of the in vitro-grown egg. These conditions could be used on a large scale to optimize the production of redclaw crayfish.http://www.sciencedirect.com/science/article/pii/S2405844023080039Cherax quadricarinatusIn vitro eggsHatching and survival ratesPhysiological biochemistry |
| spellingShingle | Shun Cheng Man Liu Yong-chun Wei Mei-li Chi Jian-bo Zheng Wen-ping Jiang Shi-li Liu Xiao-ying Hang Miao Peng Fei Li Dan-li Wang Effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggs Heliyon Cherax quadricarinatus In vitro eggs Hatching and survival rates Physiological biochemistry |
| title | Effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggs |
| title_full | Effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggs |
| title_fullStr | Effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggs |
| title_full_unstemmed | Effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggs |
| title_short | Effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggs |
| title_sort | effects of different conditions on the artificial incubation effect and physiological indexes of redclaw crayfish eggs |
| topic | Cherax quadricarinatus In vitro eggs Hatching and survival rates Physiological biochemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023080039 |
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