The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failure
PurposePulmonary arterial hypertension (PAH) leads to right ventricular failure (RVF) as well as an increase in pulmonary vascular resistance. Our purpose was to study the effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline (MCT)-induced RVF.MethodsThe rats were dist...
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| Format: | Article |
| Language: | English |
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Korean Pediatric Society
2016-06-01
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| Series: | Korean Journal of Pediatrics |
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| Online Access: | http://kjp.or.kr/upload/pdf/kjped-59-262.pdf |
| _version_ | 1818623603912998912 |
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| author | Hyun Kyung Bae Hyeryon Lee Kwan Chang Kim Young Mi Hong |
| author_facet | Hyun Kyung Bae Hyeryon Lee Kwan Chang Kim Young Mi Hong |
| author_sort | Hyun Kyung Bae |
| collection | DOAJ |
| description | PurposePulmonary arterial hypertension (PAH) leads to right ventricular failure (RVF) as well as an increase in pulmonary vascular resistance. Our purpose was to study the effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline (MCT)-induced RVF.MethodsThe rats were distributed randomly into 3 groups. The control (C) group, the monocrotaline (M) group (MCT 60 mg/kg) and the sildenafil (S) group (MCT 60 mg/kg+ sildenafil 30 mg/kg/day for 28 days). Masson Trichrome staining was used for heart tissues. Western blot analysis and immunohistochemical staining were performed.ResultsThe mean right ventricular pressure (RVP) was significantly lower in the S group at weeks 1, 2, and 4. The number of intra-acinar arteries and the medial wall thickness of the pulmonary arterioles significantly lessened in the S group at week 4. The collagen content also decreased in heart tissues in the S group at week 4. Protein expression levels of B-cell lymphoma-2 (Bcl-2)-associated X, caspase-3, Bcl-2, interleukin (IL)-6, matrix metalloproteinase (MMP)-2, endothelial nitric oxide synthase (eNOS), endothelin (ET)-1 and ET receptor A (ERA) in lung tissues greatly decreased in the S group at week 4 according to immunohistochemical staining. According to Western blotting, protein expression levels of troponin I, brain natriuretic peptide, caspase-3, Bcl-2, tumor necrosis factor-α, IL-6, MMP-2, eNOS, ET-1, and ERA in heart tissues greatly diminished in the S group at week 4.ConclusionSildenafil alleviated right ventricular hypertrophy and mean RVP. These data suggest that sildenafil improves right ventricular function. |
| first_indexed | 2024-12-16T18:43:42Z |
| format | Article |
| id | doaj.art-72c7959facc1499c9928b002bc7e33a3 |
| institution | Directory Open Access Journal |
| issn | 1738-1061 2092-7258 |
| language | English |
| last_indexed | 2024-12-16T18:43:42Z |
| publishDate | 2016-06-01 |
| publisher | Korean Pediatric Society |
| record_format | Article |
| series | Korean Journal of Pediatrics |
| spelling | doaj.art-72c7959facc1499c9928b002bc7e33a32022-12-21T22:20:56ZengKorean Pediatric SocietyKorean Journal of Pediatrics1738-10612092-72582016-06-0159626227010.3345/kjp.2016.59.6.26220125550548The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failureHyun Kyung Bae0Hyeryon Lee1Kwan Chang Kim2Young Mi Hong3Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea.Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea.Department of Thoracic and Cardiovascular Surgery, Ewha Womans University School of Medicine, Seoul, Korea.Department of Pediatrics, Ewha Womans University School of Medicine, Seoul, Korea.PurposePulmonary arterial hypertension (PAH) leads to right ventricular failure (RVF) as well as an increase in pulmonary vascular resistance. Our purpose was to study the effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline (MCT)-induced RVF.MethodsThe rats were distributed randomly into 3 groups. The control (C) group, the monocrotaline (M) group (MCT 60 mg/kg) and the sildenafil (S) group (MCT 60 mg/kg+ sildenafil 30 mg/kg/day for 28 days). Masson Trichrome staining was used for heart tissues. Western blot analysis and immunohistochemical staining were performed.ResultsThe mean right ventricular pressure (RVP) was significantly lower in the S group at weeks 1, 2, and 4. The number of intra-acinar arteries and the medial wall thickness of the pulmonary arterioles significantly lessened in the S group at week 4. The collagen content also decreased in heart tissues in the S group at week 4. Protein expression levels of B-cell lymphoma-2 (Bcl-2)-associated X, caspase-3, Bcl-2, interleukin (IL)-6, matrix metalloproteinase (MMP)-2, endothelial nitric oxide synthase (eNOS), endothelin (ET)-1 and ET receptor A (ERA) in lung tissues greatly decreased in the S group at week 4 according to immunohistochemical staining. According to Western blotting, protein expression levels of troponin I, brain natriuretic peptide, caspase-3, Bcl-2, tumor necrosis factor-α, IL-6, MMP-2, eNOS, ET-1, and ERA in heart tissues greatly diminished in the S group at week 4.ConclusionSildenafil alleviated right ventricular hypertrophy and mean RVP. These data suggest that sildenafil improves right ventricular function.http://kjp.or.kr/upload/pdf/kjped-59-262.pdfSildenafil citratePulmonary hypertensionGene expression |
| spellingShingle | Hyun Kyung Bae Hyeryon Lee Kwan Chang Kim Young Mi Hong The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failure Korean Journal of Pediatrics Sildenafil citrate Pulmonary hypertension Gene expression |
| title | The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failure |
| title_full | The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failure |
| title_fullStr | The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failure |
| title_full_unstemmed | The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failure |
| title_short | The effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline-induced right ventricular failure |
| title_sort | effect of sildenafil on right ventricular remodeling in a rat model of monocrotaline induced right ventricular failure |
| topic | Sildenafil citrate Pulmonary hypertension Gene expression |
| url | http://kjp.or.kr/upload/pdf/kjped-59-262.pdf |
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