MiR-138-5p Upregulation during Neuronal Maturation Parallels with an Increase in Neuronal Survival
Neuronal maturation is a process that plays a key role in the development and regeneration of the central nervous system. Although embryonic brain development and neurodegeneration have received considerable attention, the events that govern postnatal neuronal maturation are less understood. Among t...
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/22/16509 |
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| author | María Asunción Barreda-Manso Altea Soto Teresa Muñoz-Galdeano David Reigada Manuel Nieto-Díaz Rodrigo M. Maza |
| author_facet | María Asunción Barreda-Manso Altea Soto Teresa Muñoz-Galdeano David Reigada Manuel Nieto-Díaz Rodrigo M. Maza |
| author_sort | María Asunción Barreda-Manso |
| collection | DOAJ |
| description | Neuronal maturation is a process that plays a key role in the development and regeneration of the central nervous system. Although embryonic brain development and neurodegeneration have received considerable attention, the events that govern postnatal neuronal maturation are less understood. Among the mechanisms influencing such neuronal maturation processes, apoptosis plays a key role. Several regulators have been described to modulate apoptosis, including post-transcriptional regulation by microRNAs. This study aimed to analyze endogenous expression changes of miR-138-5p, as well as its main validated pro-apoptotic target caspase3, during the maturation of neuronal cultures and their response under apoptotic challenge. Our results point out that the observed opposite expression of miR-138-5p and its target caspase3 might modulate apoptosis favoring neuronal survival at distinct maturation stages. The unchanged expression of miR-138-5p in mature neurons contrasts with the significant downregulation in immature neurons upon apoptotic stimulation. Similarly, immunoblot and individual cellular assays confirmed that during maturation, not only the expression but processing of CASP-3 and caspase activity is reduced after apoptotic stimulation which results in a reduction of neuronal death. Further studies would be needed to determine a more detailed role of miR-138-5p in apoptosis during neuronal maturation and the synergistic action of several microRNAs acting cooperatively on caspase3 or other apoptotic targets. |
| first_indexed | 2024-03-09T16:45:02Z |
| format | Article |
| id | doaj.art-72c8224f2bc545ecb1374131910649fb |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:45:02Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-72c8224f2bc545ecb1374131910649fb2023-11-24T14:48:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221650910.3390/ijms242216509MiR-138-5p Upregulation during Neuronal Maturation Parallels with an Increase in Neuronal SurvivalMaría Asunción Barreda-Manso0Altea Soto1Teresa Muñoz-Galdeano2David Reigada3Manuel Nieto-Díaz4Rodrigo M. Maza5Research Unit, Molecular Neuroprotection Group, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainResearch Unit, Molecular Neuroprotection Group, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainResearch Unit, Molecular Neuroprotection Group, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainResearch Unit, Molecular Neuroprotection Group, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainResearch Unit, Molecular Neuroprotection Group, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainResearch Unit, Molecular Neuroprotection Group, Hospital Nacional de Parapléjicos, SESCAM, 45071 Toledo, SpainNeuronal maturation is a process that plays a key role in the development and regeneration of the central nervous system. Although embryonic brain development and neurodegeneration have received considerable attention, the events that govern postnatal neuronal maturation are less understood. Among the mechanisms influencing such neuronal maturation processes, apoptosis plays a key role. Several regulators have been described to modulate apoptosis, including post-transcriptional regulation by microRNAs. This study aimed to analyze endogenous expression changes of miR-138-5p, as well as its main validated pro-apoptotic target caspase3, during the maturation of neuronal cultures and their response under apoptotic challenge. Our results point out that the observed opposite expression of miR-138-5p and its target caspase3 might modulate apoptosis favoring neuronal survival at distinct maturation stages. The unchanged expression of miR-138-5p in mature neurons contrasts with the significant downregulation in immature neurons upon apoptotic stimulation. Similarly, immunoblot and individual cellular assays confirmed that during maturation, not only the expression but processing of CASP-3 and caspase activity is reduced after apoptotic stimulation which results in a reduction of neuronal death. Further studies would be needed to determine a more detailed role of miR-138-5p in apoptosis during neuronal maturation and the synergistic action of several microRNAs acting cooperatively on caspase3 or other apoptotic targets.https://www.mdpi.com/1422-0067/24/22/16509miR-138-5pmaturationneuroprotectionapoptosisneuronal death |
| spellingShingle | María Asunción Barreda-Manso Altea Soto Teresa Muñoz-Galdeano David Reigada Manuel Nieto-Díaz Rodrigo M. Maza MiR-138-5p Upregulation during Neuronal Maturation Parallels with an Increase in Neuronal Survival International Journal of Molecular Sciences miR-138-5p maturation neuroprotection apoptosis neuronal death |
| title | MiR-138-5p Upregulation during Neuronal Maturation Parallels with an Increase in Neuronal Survival |
| title_full | MiR-138-5p Upregulation during Neuronal Maturation Parallels with an Increase in Neuronal Survival |
| title_fullStr | MiR-138-5p Upregulation during Neuronal Maturation Parallels with an Increase in Neuronal Survival |
| title_full_unstemmed | MiR-138-5p Upregulation during Neuronal Maturation Parallels with an Increase in Neuronal Survival |
| title_short | MiR-138-5p Upregulation during Neuronal Maturation Parallels with an Increase in Neuronal Survival |
| title_sort | mir 138 5p upregulation during neuronal maturation parallels with an increase in neuronal survival |
| topic | miR-138-5p maturation neuroprotection apoptosis neuronal death |
| url | https://www.mdpi.com/1422-0067/24/22/16509 |
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