Accomplishment of a protocol for simultaneous detection of 14 intestinal bacterial pathogens based on PCR-Reverse Dot Blot
Food poisoning, caused by a bacterial infection, consequently led to a wide range of infections and endanger to public health, has been considered as a big concern in the world. Therefore, it is an urgent demand for the clinical laboratory to exactly identify infectious bacteria. In our previous stu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE
2018-10-01
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| Series: | Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ |
| Subjects: | |
| Online Access: | https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/803 |
| _version_ | 1818450607049015296 |
|---|---|
| author | Hồ Thị Thanh Thủy Nguyễn Văn Trường Nguyễn Bảo Toàn Lao Đức Thuận Trương Kim Phượng Lê Huyền Ái Thúy |
| author_facet | Hồ Thị Thanh Thủy Nguyễn Văn Trường Nguyễn Bảo Toàn Lao Đức Thuận Trương Kim Phượng Lê Huyền Ái Thúy |
| author_sort | Hồ Thị Thanh Thủy |
| collection | DOAJ |
| description | Food poisoning, caused by a bacterial infection, consequently led to a wide range of infections and endanger to public health, has been considered as a big concern in the world. Therefore, it is an urgent demand for the clinical laboratory to exactly identify infectious bacteria. In our previous study, we successfully published a protocol based on the PCR-Reverse Dot Blot (PCR-RDB) to determine simultaneously 12 bacterial intestinal pathogens, including Bacillus cereus, Clostridium botulinum, Clostridium perfringen, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157: H7, Salmonella spp., Shigella spp.., Vibrio cholerae, Vibrio parahaemolyticus, Yersinia enterocolitica and Brucella spp. In this study, we continuously developed our published protocol by designing additional probes: positive control probe, negative control probe, color control probe and background signal controller. Moreover, concerning the clinical demand, the supplement of two designed probes, which detected Campylobacter jejuni và Yersinia enterocolitica O:8 caused intestinal infected diseases mainly in children, was necessary. As a result, this completed PCR-RDB protocol can simultaneously detect a total of 14 intestinal bacterial pathogens within high specificity and the sensitivity of 102 copies/ml. For the protocol confirmation, it was tested by 30 fecal samples and the results completely match with the commercial kit PowerCheckTM 20 Pathogen Multiplex Real-time PCR Kit (Korea). |
| first_indexed | 2024-12-14T20:53:59Z |
| format | Article |
| id | doaj.art-72c91b1e00b245869146a1d03ee4ba9c |
| institution | Directory Open Access Journal |
| issn | 2734-9322 2734-9594 |
| language | English |
| last_indexed | 2024-12-14T20:53:59Z |
| publishDate | 2018-10-01 |
| publisher | HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE |
| record_format | Article |
| series | Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ |
| spelling | doaj.art-72c91b1e00b245869146a1d03ee4ba9c2022-12-21T22:47:43ZengHO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCETạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ2734-93222734-95942018-10-01131668010.46223/HCMCOUJS.tech.vi.13.1.803.2018675Accomplishment of a protocol for simultaneous detection of 14 intestinal bacterial pathogens based on PCR-Reverse Dot BlotHồ Thị Thanh Thủy0Nguyễn Văn Trường1Nguyễn Bảo Toàn2Lao Đức Thuận3Trương Kim Phượng4Lê Huyền Ái Thúy5Công ty Cổ phần và Công nghệ Việt ÁCông ty Cổ phần và Công nghệ Việt ÁTrung tâm chẩn đoán Y khoa Medic, Thành phố Hồ Chí MinhTrường Đại học Mở Thành phố Hồ Chí MinhTrường Đại học Mở Thành phố Hồ Chí MinhTrường Đại học Mở Thành phố Hồ Chí MinhFood poisoning, caused by a bacterial infection, consequently led to a wide range of infections and endanger to public health, has been considered as a big concern in the world. Therefore, it is an urgent demand for the clinical laboratory to exactly identify infectious bacteria. In our previous study, we successfully published a protocol based on the PCR-Reverse Dot Blot (PCR-RDB) to determine simultaneously 12 bacterial intestinal pathogens, including Bacillus cereus, Clostridium botulinum, Clostridium perfringen, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli O157: H7, Salmonella spp., Shigella spp.., Vibrio cholerae, Vibrio parahaemolyticus, Yersinia enterocolitica and Brucella spp. In this study, we continuously developed our published protocol by designing additional probes: positive control probe, negative control probe, color control probe and background signal controller. Moreover, concerning the clinical demand, the supplement of two designed probes, which detected Campylobacter jejuni và Yersinia enterocolitica O:8 caused intestinal infected diseases mainly in children, was necessary. As a result, this completed PCR-RDB protocol can simultaneously detect a total of 14 intestinal bacterial pathogens within high specificity and the sensitivity of 102 copies/ml. For the protocol confirmation, it was tested by 30 fecal samples and the results completely match with the commercial kit PowerCheckTM 20 Pathogen Multiplex Real-time PCR Kit (Korea).https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/803campylobacter jejunipcr-reverse dot blotvi khuẩn gây bệnh đường ruộtyersinia enterocolitica o:8 |
| spellingShingle | Hồ Thị Thanh Thủy Nguyễn Văn Trường Nguyễn Bảo Toàn Lao Đức Thuận Trương Kim Phượng Lê Huyền Ái Thúy Accomplishment of a protocol for simultaneous detection of 14 intestinal bacterial pathogens based on PCR-Reverse Dot Blot Tạp chí Khoa học Đại học Mở Thành phố Hồ Chí Minh - Kỹ thuật và Công nghệ campylobacter jejuni pcr-reverse dot blot vi khuẩn gây bệnh đường ruột yersinia enterocolitica o:8 |
| title | Accomplishment of a protocol for simultaneous detection of 14 intestinal bacterial pathogens based on PCR-Reverse Dot Blot |
| title_full | Accomplishment of a protocol for simultaneous detection of 14 intestinal bacterial pathogens based on PCR-Reverse Dot Blot |
| title_fullStr | Accomplishment of a protocol for simultaneous detection of 14 intestinal bacterial pathogens based on PCR-Reverse Dot Blot |
| title_full_unstemmed | Accomplishment of a protocol for simultaneous detection of 14 intestinal bacterial pathogens based on PCR-Reverse Dot Blot |
| title_short | Accomplishment of a protocol for simultaneous detection of 14 intestinal bacterial pathogens based on PCR-Reverse Dot Blot |
| title_sort | accomplishment of a protocol for simultaneous detection of 14 intestinal bacterial pathogens based on pcr reverse dot blot |
| topic | campylobacter jejuni pcr-reverse dot blot vi khuẩn gây bệnh đường ruột yersinia enterocolitica o:8 |
| url | https://journalofscience.ou.edu.vn/index.php/tech-vi/article/view/803 |
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