The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro
Abstract Background Lysophosphatidic acid-supplemented culture medium significantly increases the oocyte maturation rate in vitro. However, potential targets and pathways involved remain unknown. Methods A total of 43 women, who underwent cesarean section and aged between 18 and 35 years with good h...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | Reproductive Biology and Endocrinology |
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| Online Access: | https://doi.org/10.1186/s12958-021-00771-8 |
| _version_ | 1819100390264668160 |
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| author | Qigui Xie Yaxin Xing Jianhong Zhou Ling Wang Jie Wu Ri-Cheng Chian |
| author_facet | Qigui Xie Yaxin Xing Jianhong Zhou Ling Wang Jie Wu Ri-Cheng Chian |
| author_sort | Qigui Xie |
| collection | DOAJ |
| description | Abstract Background Lysophosphatidic acid-supplemented culture medium significantly increases the oocyte maturation rate in vitro. However, potential targets and pathways involved remain unknown. Methods A total of 43 women, who underwent cesarean section and aged between 18 and 35 years with good health, were included in this study. Immature oocytes were obtained and cultured with 10 µM lysophosphatidic acid. After culture, oocyte maturation was assessed and oocytes and cumulus cells were collected for RNA sequencing. Hierarchical indexing for spliced alignment of transcripts 2 method was used to align clean reads to the human genome. The featureCounts and edgeR package were used to calculate gene expression and analyze differences between groups respectively. ClusterProfiler program was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. Results Oocyte maturation rate increased significantly following 48 h culture with lysophosphatidic acid. In cumulus cells, Gene Ontology analysis revealed the top 20 items enriched by upregulated genes and downregulated genes respectively; Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated genes in the treatment group were enriched in TNF signaling and insulin secretion pathways and downregulated genes were enriched in TNF signaling and cell adhesion molecules. In oocytes, Gene Ontology analysis revealed the top 20 items enriched by upregulated genes and downregulated genes respectively; Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated genes in the treatment group were enriched in MAPK signaling, gap junction, and cell cycle pathways and downregulated genes were enriched in MAPK signaling, estrogen signaling, RAP1 signaling, and gap junction pathways. Conclusions Lysophosphatidic acid in culture medium enhances human oocyte maturation in vitro and the identified some potential pathways may associate with oocyte maturation. |
| first_indexed | 2024-12-22T01:02:01Z |
| format | Article |
| id | doaj.art-72ccaf2034bd404ea53984a222a5e179 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-12-22T01:02:01Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-72ccaf2034bd404ea53984a222a5e1792022-12-21T18:44:10ZengBMCReproductive Biology and Endocrinology1477-78272021-06-011911810.1186/s12958-021-00771-8The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitroQigui Xie0Yaxin Xing1Jianhong Zhou2Ling Wang3Jie Wu4Ri-Cheng Chian5Department of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Nanjing Medical UniversityCenter for Reproductive Medicine, Shanghai Tenth People’s Hospital of Tongji UniversityDepartment of Obstetrics and Gynecology, Shanghai Tenth People’s Hospital, Nanjing Medical UniversityCenter for Reproductive Medicine, Shanghai Tenth People’s Hospital of Tongji UniversityDepartment of Obstetrics and Gynecology, Jiangsu Province Hospital, The First Affiliated Hospital with Nanjing Medical University, Nanjing Medical UniversityCenter for Reproductive Medicine, Shanghai Tenth People’s Hospital of Tongji UniversityAbstract Background Lysophosphatidic acid-supplemented culture medium significantly increases the oocyte maturation rate in vitro. However, potential targets and pathways involved remain unknown. Methods A total of 43 women, who underwent cesarean section and aged between 18 and 35 years with good health, were included in this study. Immature oocytes were obtained and cultured with 10 µM lysophosphatidic acid. After culture, oocyte maturation was assessed and oocytes and cumulus cells were collected for RNA sequencing. Hierarchical indexing for spliced alignment of transcripts 2 method was used to align clean reads to the human genome. The featureCounts and edgeR package were used to calculate gene expression and analyze differences between groups respectively. ClusterProfiler program was used to perform Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. Results Oocyte maturation rate increased significantly following 48 h culture with lysophosphatidic acid. In cumulus cells, Gene Ontology analysis revealed the top 20 items enriched by upregulated genes and downregulated genes respectively; Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated genes in the treatment group were enriched in TNF signaling and insulin secretion pathways and downregulated genes were enriched in TNF signaling and cell adhesion molecules. In oocytes, Gene Ontology analysis revealed the top 20 items enriched by upregulated genes and downregulated genes respectively; Kyoto Encyclopedia of Genes and Genomes analysis showed that upregulated genes in the treatment group were enriched in MAPK signaling, gap junction, and cell cycle pathways and downregulated genes were enriched in MAPK signaling, estrogen signaling, RAP1 signaling, and gap junction pathways. Conclusions Lysophosphatidic acid in culture medium enhances human oocyte maturation in vitro and the identified some potential pathways may associate with oocyte maturation.https://doi.org/10.1186/s12958-021-00771-8OocytesCumulus cellsIn vitro maturationLysophosphatidic acidFertility |
| spellingShingle | Qigui Xie Yaxin Xing Jianhong Zhou Ling Wang Jie Wu Ri-Cheng Chian The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro Reproductive Biology and Endocrinology Oocytes Cumulus cells In vitro maturation Lysophosphatidic acid Fertility |
| title | The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro |
| title_full | The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro |
| title_fullStr | The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro |
| title_full_unstemmed | The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro |
| title_short | The effect of lysophosphatidic acid-supplemented culture medium on human immature oocytes matured in vitro |
| title_sort | effect of lysophosphatidic acid supplemented culture medium on human immature oocytes matured in vitro |
| topic | Oocytes Cumulus cells In vitro maturation Lysophosphatidic acid Fertility |
| url | https://doi.org/10.1186/s12958-021-00771-8 |
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