Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2
Abstract Background Without sufficient alternatives to crude oil, as demand continues to rise, the global economy will undergo a drastic decline as oil prices explode. Dependence on crude oil and growing environmental impairment must eventually be overcome by creating a sustainable and profitable al...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-020-01676-1 |
| _version_ | 1818554564430790656 |
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| author | Kiruthika Thangavelu Pugalendhi Sundararaju Naganandhini Srinivasan Iniyakumar Muniraj Sivakumar Uthandi |
| author_facet | Kiruthika Thangavelu Pugalendhi Sundararaju Naganandhini Srinivasan Iniyakumar Muniraj Sivakumar Uthandi |
| author_sort | Kiruthika Thangavelu |
| collection | DOAJ |
| description | Abstract Background Without sufficient alternatives to crude oil, as demand continues to rise, the global economy will undergo a drastic decline as oil prices explode. Dependence on crude oil and growing environmental impairment must eventually be overcome by creating a sustainable and profitable alternative based on renewable and accessible feedstock. One of the promising solutions for the current and near-future is the substitution of fossil fuels with sustainable liquid feedstock for biofuel production. Among the different renewable liquid feedstock’s studied, wastewater is the least explored one for biodiesel production. Sago wastewater is the byproduct of the cassava processing industry and has starch content ranging from 4 to 7%. The present investigation was aimed to produce microbial lipids from oleaginous yeast, Candida tropicalis ASY2 for use as biodiesel feedstock and simultaneously decontaminate the sago processing wastewater for reuse. Initial screening of oleaginous yeast to find an efficient amylolytic with maximum lipid productivity resulted in a potent oleaginous yeast strain, C. tropicalis ASY2, that utilizes SWW as a substrate. Shake flask experiments are conducted over a fermentation time of 240 h to determine a suitable fatty acid composition using GC-FID for biodiesel production with simultaneous removal of SWW pollutants using ASY2. Results The maximum biomass of 0.021 g L−1 h−1 and lipid productivity of 0.010 g L−1 h−1 was recorded in SWW with lipid content of 49%. The yeast strain degraded cyanide in SWW (79%) and also removed chemical oxygen demand (COD), biological oxygen demand (BOD), nitrate (NO3), ammoniacal (NH4), and phosphate (PO4) ions (84%, 92%, 100%, 98%, and 85%, respectively). GC-FID analysis of fatty acid methyl esters (FAME) revealed high oleic acid content (41.33%), which is one of the primary fatty acids for biodiesel production. Conclusions It is evident that the present study provides an innovative and ecologically sustainable technology that generates valuable fuel, biodiesel using SWW as a substrate and decontaminates for reuse. |
| first_indexed | 2024-12-12T09:41:33Z |
| format | Article |
| id | doaj.art-72d03d7ed2c14f12887df8fd762a448e |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-12-12T09:41:33Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-72d03d7ed2c14f12887df8fd762a448e2022-12-22T00:28:32ZengBMCBiotechnology for Biofuels1754-68342020-03-0113111410.1186/s13068-020-01676-1Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2Kiruthika Thangavelu0Pugalendhi Sundararaju1Naganandhini Srinivasan2Iniyakumar Muniraj3Sivakumar Uthandi4Department of Renewable Energy Engineering, Agricultural Engineering College and Research InstituteDepartment of Renewable Energy Engineering, Agricultural Engineering College and Research InstituteBiocatalysts Lab, Department of Agricultural Microbiology, Tamil Nadu Agricultural UniversityDepartment of Crop Management, Kumaraguru Institute of AgricultureBiocatalysts Lab, Department of Agricultural Microbiology, Tamil Nadu Agricultural UniversityAbstract Background Without sufficient alternatives to crude oil, as demand continues to rise, the global economy will undergo a drastic decline as oil prices explode. Dependence on crude oil and growing environmental impairment must eventually be overcome by creating a sustainable and profitable alternative based on renewable and accessible feedstock. One of the promising solutions for the current and near-future is the substitution of fossil fuels with sustainable liquid feedstock for biofuel production. Among the different renewable liquid feedstock’s studied, wastewater is the least explored one for biodiesel production. Sago wastewater is the byproduct of the cassava processing industry and has starch content ranging from 4 to 7%. The present investigation was aimed to produce microbial lipids from oleaginous yeast, Candida tropicalis ASY2 for use as biodiesel feedstock and simultaneously decontaminate the sago processing wastewater for reuse. Initial screening of oleaginous yeast to find an efficient amylolytic with maximum lipid productivity resulted in a potent oleaginous yeast strain, C. tropicalis ASY2, that utilizes SWW as a substrate. Shake flask experiments are conducted over a fermentation time of 240 h to determine a suitable fatty acid composition using GC-FID for biodiesel production with simultaneous removal of SWW pollutants using ASY2. Results The maximum biomass of 0.021 g L−1 h−1 and lipid productivity of 0.010 g L−1 h−1 was recorded in SWW with lipid content of 49%. The yeast strain degraded cyanide in SWW (79%) and also removed chemical oxygen demand (COD), biological oxygen demand (BOD), nitrate (NO3), ammoniacal (NH4), and phosphate (PO4) ions (84%, 92%, 100%, 98%, and 85%, respectively). GC-FID analysis of fatty acid methyl esters (FAME) revealed high oleic acid content (41.33%), which is one of the primary fatty acids for biodiesel production. Conclusions It is evident that the present study provides an innovative and ecologically sustainable technology that generates valuable fuel, biodiesel using SWW as a substrate and decontaminates for reuse.http://link.springer.com/article/10.1186/s13068-020-01676-1Sago wastewaterStarchOleaginous yeastLipid productionBiodiesel |
| spellingShingle | Kiruthika Thangavelu Pugalendhi Sundararaju Naganandhini Srinivasan Iniyakumar Muniraj Sivakumar Uthandi Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2 Biotechnology for Biofuels Sago wastewater Starch Oleaginous yeast Lipid production Biodiesel |
| title | Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2 |
| title_full | Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2 |
| title_fullStr | Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2 |
| title_full_unstemmed | Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2 |
| title_short | Simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using Candida tropicalis ASY2 |
| title_sort | simultaneous lipid production for biodiesel feedstock and decontamination of sago processing wastewater using candida tropicalis asy2 |
| topic | Sago wastewater Starch Oleaginous yeast Lipid production Biodiesel |
| url | http://link.springer.com/article/10.1186/s13068-020-01676-1 |
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