Blocking Abundant RNA Transcripts by High-Affinity Oligonucleotides during Transcriptome Library Preparation

Blocking Abundant RNA Transcripts by High-Affinity Oligonucleotides during Transcriptome Library Preparation

Abstract Background RNA sequencing has become the gold standard for transcriptome analysis but has an inherent limitation of challenging quantification of low-abundant transcripts. In contrast to microarray technology, RNA sequencing reads are proportionally divided in function of transcript abundan...

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Bibliographic Details
Main Authors: Celine Everaert, Jasper Verwilt, Kimberly Verniers, Niels Vandamme, Alvaro Marcos Rubio, Jo Vandesompele, Pieter Mestdagh
Format: Article
Language:English
Published: BMC 2023-03-01
Series:Biological Procedures Online
Subjects:
RNA sequencing
Oxford nanopore technologies
Single-cell RNA sequencing
Depletion
Online Access:https://doi.org/10.1186/s12575-023-00193-3

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