PTEN is a negative regulator of mitotic checkpoint complex during the cell cycle
Abstract Nuclear PTEN plays an important role during mitosis. To understand the molecular basis by which PTEN mediates mitotic progression, we examined whether PTEN regulated the formation of mitotic checkpoint complex (MCC). We observed that arsenic trioxide, a mitotic inducer, stimulated nuclear t...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | Experimental Hematology & Oncology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40164-017-0079-0 |
| _version_ | 1819065977301630976 |
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| author | Byeong H. Choi Steve Xie Wei Dai |
| author_facet | Byeong H. Choi Steve Xie Wei Dai |
| author_sort | Byeong H. Choi |
| collection | DOAJ |
| description | Abstract Nuclear PTEN plays an important role during mitosis. To understand the molecular basis by which PTEN mediates mitotic progression, we examined whether PTEN regulated the formation of mitotic checkpoint complex (MCC). We observed that arsenic trioxide, a mitotic inducer, stimulated nuclear translocation of PTEN in a time-dependent manner. PTEN physically interacted with Cdc20 and Mad2, two important components of MCC. Arsenic treatment diminished the physical association of PTEN with BubR1 and Bub3 but not with Cdc20 and Mad2. Our further studies revealed that downregulation of PTEN via RNAi enhanced formation of MCC during the cell cycle. Moreover, PTEN silencing induced chromosomal instability. Given the crucial role of PTEN in suppressing tumor development, our study strongly suggests that PTEN also functions to maintain chromosomal stability, partly through suppressing unscheduled formation of MCC. |
| first_indexed | 2024-12-21T15:55:02Z |
| format | Article |
| id | doaj.art-72d1753304be4c6c83e1521e0b964c82 |
| institution | Directory Open Access Journal |
| issn | 2162-3619 |
| language | English |
| last_indexed | 2024-12-21T15:55:02Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Experimental Hematology & Oncology |
| spelling | doaj.art-72d1753304be4c6c83e1521e0b964c822022-12-21T18:58:08ZengBMCExperimental Hematology & Oncology2162-36192017-06-01611710.1186/s40164-017-0079-0PTEN is a negative regulator of mitotic checkpoint complex during the cell cycleByeong H. Choi0Steve Xie1Wei Dai2Departments of Environmental Medicine, Biochemistry and Molecular Pharmacology, New York University Langone Medical CenterInstitute of Pathology, Kings County Hospital CenterDepartments of Environmental Medicine, Biochemistry and Molecular Pharmacology, New York University Langone Medical CenterAbstract Nuclear PTEN plays an important role during mitosis. To understand the molecular basis by which PTEN mediates mitotic progression, we examined whether PTEN regulated the formation of mitotic checkpoint complex (MCC). We observed that arsenic trioxide, a mitotic inducer, stimulated nuclear translocation of PTEN in a time-dependent manner. PTEN physically interacted with Cdc20 and Mad2, two important components of MCC. Arsenic treatment diminished the physical association of PTEN with BubR1 and Bub3 but not with Cdc20 and Mad2. Our further studies revealed that downregulation of PTEN via RNAi enhanced formation of MCC during the cell cycle. Moreover, PTEN silencing induced chromosomal instability. Given the crucial role of PTEN in suppressing tumor development, our study strongly suggests that PTEN also functions to maintain chromosomal stability, partly through suppressing unscheduled formation of MCC.http://link.springer.com/article/10.1186/s40164-017-0079-0PTENMitosisMitotic checkpoint complexNuclear localizationChromosomes |
| spellingShingle | Byeong H. Choi Steve Xie Wei Dai PTEN is a negative regulator of mitotic checkpoint complex during the cell cycle Experimental Hematology & Oncology PTEN Mitosis Mitotic checkpoint complex Nuclear localization Chromosomes |
| title | PTEN is a negative regulator of mitotic checkpoint complex during the cell cycle |
| title_full | PTEN is a negative regulator of mitotic checkpoint complex during the cell cycle |
| title_fullStr | PTEN is a negative regulator of mitotic checkpoint complex during the cell cycle |
| title_full_unstemmed | PTEN is a negative regulator of mitotic checkpoint complex during the cell cycle |
| title_short | PTEN is a negative regulator of mitotic checkpoint complex during the cell cycle |
| title_sort | pten is a negative regulator of mitotic checkpoint complex during the cell cycle |
| topic | PTEN Mitosis Mitotic checkpoint complex Nuclear localization Chromosomes |
| url | http://link.springer.com/article/10.1186/s40164-017-0079-0 |
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