Aberrant expression for microRNA is potential crucial factors of haemorrhoid
Abstract Background Haemorrhoids occur commonly and frequently in the human digestive system. There are diverse causes of haemorrhoids and their in-depth pathogenesis is still currently unclear. Methods In this study, we explored haemorrhoids from an epigenetics perspective by employing RNA-Seq for...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | Hereditas |
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| Online Access: | http://link.springer.com/article/10.1186/s41065-020-00139-9 |
| _version_ | 1818347777690697728 |
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| author | Chengkun Song Haikun Zhou Hong Lu Chunsheng Luo Chen Wang Qingming Wang Yunhua Peng Yaojie Xin Te Liu Wei Yang |
| author_facet | Chengkun Song Haikun Zhou Hong Lu Chunsheng Luo Chen Wang Qingming Wang Yunhua Peng Yaojie Xin Te Liu Wei Yang |
| author_sort | Chengkun Song |
| collection | DOAJ |
| description | Abstract Background Haemorrhoids occur commonly and frequently in the human digestive system. There are diverse causes of haemorrhoids and their in-depth pathogenesis is still currently unclear. Methods In this study, we explored haemorrhoids from an epigenetics perspective by employing RNA-Seq for comprehensive and in-depth analysis of the differences in microRNA (miRNA) transcripts between haemorrhoidal tissue and normal tissue in 48 patients with Grade II and above haemorrhoids. Results The results showed that 9 miRNAs were significantly upregulated (ratio > 3.5 and P-value < 0.01) and 16 miRNAs were significantly downregulated (ratio > 0.6 and P-value < 0.01) in haemorrhoid tissue. Subsequently, target gene prediction results showed that there were 184 potential target genes of significantly upregulated miRNAs (common to both TargetScan7.1 and MirdbV5 databases) and there were 372 potential target genes of significantly downregulated miRNAs. Gene ontology analysis results showed that the target genes of differentially expressed miRNAs in haemorrhoids are involved in regulating “cell composition” and “protein binding”. Lastly, KEGG search found that the differentially expressed miRNAs that are associated with the occurrence of haemorrhoids mainly regulate the activity of endocytosis and the synaptic vesicle cycle. Conclusions In summary, the results of high-throughput RNA-Seq screening suggested that the occurrence of haemorrhoids may be intimately associated with aberrant miRNA transcription, resulting in aberrant target gene expression and an imbalance in certain signal transduction pathways. |
| first_indexed | 2024-12-13T17:39:33Z |
| format | Article |
| id | doaj.art-72d43bd8e5124698841b25dc7eadd994 |
| institution | Directory Open Access Journal |
| issn | 1601-5223 |
| language | English |
| last_indexed | 2024-12-13T17:39:33Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj.art-72d43bd8e5124698841b25dc7eadd9942022-12-21T23:36:49ZengBMCHereditas1601-52232020-07-01157111210.1186/s41065-020-00139-9Aberrant expression for microRNA is potential crucial factors of haemorrhoidChengkun Song0Haikun Zhou1Hong Lu2Chunsheng Luo3Chen Wang4Qingming Wang5Yunhua Peng6Yaojie Xin7Te Liu8Wei Yang9Department of Anorectal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Anorectal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Anorectal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Anorectal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Anorectal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Anorectal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Anorectal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineDepartment of Otolaryngology, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineShanghai Geriatric Institute of Chinese Medicine, Shanghai University of Traditional Chinese MedicineDepartment of Anorectal Surgery, Shuguang Hospital, Shanghai University of Traditional Chinese MedicineAbstract Background Haemorrhoids occur commonly and frequently in the human digestive system. There are diverse causes of haemorrhoids and their in-depth pathogenesis is still currently unclear. Methods In this study, we explored haemorrhoids from an epigenetics perspective by employing RNA-Seq for comprehensive and in-depth analysis of the differences in microRNA (miRNA) transcripts between haemorrhoidal tissue and normal tissue in 48 patients with Grade II and above haemorrhoids. Results The results showed that 9 miRNAs were significantly upregulated (ratio > 3.5 and P-value < 0.01) and 16 miRNAs were significantly downregulated (ratio > 0.6 and P-value < 0.01) in haemorrhoid tissue. Subsequently, target gene prediction results showed that there were 184 potential target genes of significantly upregulated miRNAs (common to both TargetScan7.1 and MirdbV5 databases) and there were 372 potential target genes of significantly downregulated miRNAs. Gene ontology analysis results showed that the target genes of differentially expressed miRNAs in haemorrhoids are involved in regulating “cell composition” and “protein binding”. Lastly, KEGG search found that the differentially expressed miRNAs that are associated with the occurrence of haemorrhoids mainly regulate the activity of endocytosis and the synaptic vesicle cycle. Conclusions In summary, the results of high-throughput RNA-Seq screening suggested that the occurrence of haemorrhoids may be intimately associated with aberrant miRNA transcription, resulting in aberrant target gene expression and an imbalance in certain signal transduction pathways.http://link.springer.com/article/10.1186/s41065-020-00139-9HaemorrhoidsmicroRNARNA-SeqSignal transduction pathways |
| spellingShingle | Chengkun Song Haikun Zhou Hong Lu Chunsheng Luo Chen Wang Qingming Wang Yunhua Peng Yaojie Xin Te Liu Wei Yang Aberrant expression for microRNA is potential crucial factors of haemorrhoid Hereditas Haemorrhoids microRNA RNA-Seq Signal transduction pathways |
| title | Aberrant expression for microRNA is potential crucial factors of haemorrhoid |
| title_full | Aberrant expression for microRNA is potential crucial factors of haemorrhoid |
| title_fullStr | Aberrant expression for microRNA is potential crucial factors of haemorrhoid |
| title_full_unstemmed | Aberrant expression for microRNA is potential crucial factors of haemorrhoid |
| title_short | Aberrant expression for microRNA is potential crucial factors of haemorrhoid |
| title_sort | aberrant expression for microrna is potential crucial factors of haemorrhoid |
| topic | Haemorrhoids microRNA RNA-Seq Signal transduction pathways |
| url | http://link.springer.com/article/10.1186/s41065-020-00139-9 |
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