Neuropharmacological Activity of the Acetonic Extract of <i>Malpighia mexicana</i> A. Juss. and Its Phytochemical Profile
Mental and neurological disorders are conditions that affect thoughts, emotions, behavior, and relationships. <i>Malpighia mexicana</i> A. Juss. is a plant used in Mexican traditional medicine for the treatment of such disorders. This work aimed to investigate the antidepressant, anxioly...
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MDPI AG
2023-10-01
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| Series: | Scientia Pharmaceutica |
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| Online Access: | https://www.mdpi.com/2218-0532/91/4/47 |
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| author | Dante Avilés-Montes David Osvaldo Salinas-Sánchez César Sotelo-Leyva Alejandro Zamilpa Franceli Itzel Batalla-Martinez Rodolfo Abarca-Vargas Juan Manuel Rivas-González Óscar Dorado Rodolfo Figueroa-Brito Vera L. Petricevich Dulce Lourdes Morales-Ferra Manasés González-Cortazar |
| author_facet | Dante Avilés-Montes David Osvaldo Salinas-Sánchez César Sotelo-Leyva Alejandro Zamilpa Franceli Itzel Batalla-Martinez Rodolfo Abarca-Vargas Juan Manuel Rivas-González Óscar Dorado Rodolfo Figueroa-Brito Vera L. Petricevich Dulce Lourdes Morales-Ferra Manasés González-Cortazar |
| author_sort | Dante Avilés-Montes |
| collection | DOAJ |
| description | Mental and neurological disorders are conditions that affect thoughts, emotions, behavior, and relationships. <i>Malpighia mexicana</i> A. Juss. is a plant used in Mexican traditional medicine for the treatment of such disorders. This work aimed to investigate the antidepressant, anxiolytic, sedative, hypnotic, and anticonvulsant effects of the acetonic extract (MmAE) of <i>M. mexicana</i> and its fractions (F3, F4-10, F14) using the forced swimming test (FST), elevated plus maze (EPM), open field test (OFT), pentobarbital-induced sleep test (PBTt), and pentylenetetrazol-induced seizure test (PTZt). MmAE, F3, F4-10, F14, and vehicle were administrated orally 24, 18, and 1 h prior to the evaluations. Imipramine (15 mg/kg, <i>p.o.</i>) was administrated 1 h prior to the evaluations as a positive control for the FST, while diazepam (1 mg/kg, <i>p.o.</i>) was administrated 1 h prior to the evaluations as a positive control for the EPM, OFT, PBTt, and PTZt. MmAE had an anxiolytic effect; MmAE and F3, F4-10, and F14 showed an antidepressant effect, sedative effect, hypnotic effect, and anticonvulsant effect. Using HPLC, we identified the compounds quercetin 3-<i>O</i>-rutinoside (<b>1</b>), kaempferol 3-<i>O</i>-glucoside (<b>2</b>), luteolin 7-<i>O</i>-glucoside (<b>3</b>), quercetin (<b>4</b>), and kaempferol (<b>5</b>) in MmAE and compounds (<b>1</b>), (<b>2</b>), and (<b>3</b>) in F14. Using GC-MS, we identified α-tocopherol, phytol, and β-amyrin in F3; β-tocopherol, phytol, β-sitosterol, and β-amyrin in F4-10; and α- tocopherol, phytol, β-sitosterol, and β-amyrin in F4-10. The neuropharmacological effects found in this work may be due to the presence of vitamins, phytosterols, terpenes, and flavonoids. This research requires further study to clarify the mechanisms of action of the identified compounds. |
| first_indexed | 2024-03-08T20:23:04Z |
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| issn | 0036-8709 2218-0532 |
| language | English |
| last_indexed | 2024-03-08T20:23:04Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
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| series | Scientia Pharmaceutica |
| spelling | doaj.art-72d440b27fcb4bae989b3277479d2eed2023-12-22T14:39:53ZengMDPI AGScientia Pharmaceutica0036-87092218-05322023-10-019144710.3390/scipharm91040047Neuropharmacological Activity of the Acetonic Extract of <i>Malpighia mexicana</i> A. Juss. and Its Phytochemical ProfileDante Avilés-Montes0David Osvaldo Salinas-Sánchez1César Sotelo-Leyva2Alejandro Zamilpa3Franceli Itzel Batalla-Martinez4Rodolfo Abarca-Vargas5Juan Manuel Rivas-González6Óscar Dorado7Rodolfo Figueroa-Brito8Vera L. Petricevich9Dulce Lourdes Morales-Ferra10Manasés González-Cortazar11Facultad de Ciencias Biológicas, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, MexicoCentro de Investigación en Biodiversidad y Conservación, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col. Chamilpa, Cuernavaca 62209, Morelos, MexicoFacultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria Sur, Chilpancingo 39000, Guerrero, MexicoCentro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, MexicoEscuela de Estudios Superiores del Jicarero, Universidad Autónoma del Estado de Morelos, Galeana-Tequesquitengo S/N, Col. El Jicarero, Jojutla 62909, Morelos, MexicoFacultad de Medicina, Universidad Autónoma del Estado de Morelos, Leñeros, Esquina Iztaccíhuatl S/N, Col. Volcanes, Cuernavaca 62350, Morelos, MexicoEscuela de Estudios Superiores del Jicarero, Universidad Autónoma del Estado de Morelos, Galeana-Tequesquitengo S/N, Col. El Jicarero, Jojutla 62909, Morelos, MexicoCentro de Educación Ambiental e Investigación Sierra de Huautla, Universidad Autónoma del Estado de Morelos, Cuernavaca 62209, Morelos, MexicoCentro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, Carretera Yautepec-Jojutla Km 6, AP 24, San Isidro, Yautepec 62731, Morelos, MexicoFacultad de Medicina, Universidad Autónoma del Estado de Morelos, Leñeros, Esquina Iztaccíhuatl S/N, Col. Volcanes, Cuernavaca 62350, Morelos, MexicoCentro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina No. 1, Col. Centro, Xochitepec 62790, Morelos, MexicoFacultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas S/N, Ciudad Universitaria Sur, Chilpancingo 39000, Guerrero, MexicoMental and neurological disorders are conditions that affect thoughts, emotions, behavior, and relationships. <i>Malpighia mexicana</i> A. Juss. is a plant used in Mexican traditional medicine for the treatment of such disorders. This work aimed to investigate the antidepressant, anxiolytic, sedative, hypnotic, and anticonvulsant effects of the acetonic extract (MmAE) of <i>M. mexicana</i> and its fractions (F3, F4-10, F14) using the forced swimming test (FST), elevated plus maze (EPM), open field test (OFT), pentobarbital-induced sleep test (PBTt), and pentylenetetrazol-induced seizure test (PTZt). MmAE, F3, F4-10, F14, and vehicle were administrated orally 24, 18, and 1 h prior to the evaluations. Imipramine (15 mg/kg, <i>p.o.</i>) was administrated 1 h prior to the evaluations as a positive control for the FST, while diazepam (1 mg/kg, <i>p.o.</i>) was administrated 1 h prior to the evaluations as a positive control for the EPM, OFT, PBTt, and PTZt. MmAE had an anxiolytic effect; MmAE and F3, F4-10, and F14 showed an antidepressant effect, sedative effect, hypnotic effect, and anticonvulsant effect. Using HPLC, we identified the compounds quercetin 3-<i>O</i>-rutinoside (<b>1</b>), kaempferol 3-<i>O</i>-glucoside (<b>2</b>), luteolin 7-<i>O</i>-glucoside (<b>3</b>), quercetin (<b>4</b>), and kaempferol (<b>5</b>) in MmAE and compounds (<b>1</b>), (<b>2</b>), and (<b>3</b>) in F14. Using GC-MS, we identified α-tocopherol, phytol, and β-amyrin in F3; β-tocopherol, phytol, β-sitosterol, and β-amyrin in F4-10; and α- tocopherol, phytol, β-sitosterol, and β-amyrin in F4-10. The neuropharmacological effects found in this work may be due to the presence of vitamins, phytosterols, terpenes, and flavonoids. This research requires further study to clarify the mechanisms of action of the identified compounds.https://www.mdpi.com/2218-0532/91/4/47antidepressantanxiolyticsedativehypnoticanticonvulsant<i>Malpighia mexicana</i> |
| spellingShingle | Dante Avilés-Montes David Osvaldo Salinas-Sánchez César Sotelo-Leyva Alejandro Zamilpa Franceli Itzel Batalla-Martinez Rodolfo Abarca-Vargas Juan Manuel Rivas-González Óscar Dorado Rodolfo Figueroa-Brito Vera L. Petricevich Dulce Lourdes Morales-Ferra Manasés González-Cortazar Neuropharmacological Activity of the Acetonic Extract of <i>Malpighia mexicana</i> A. Juss. and Its Phytochemical Profile Scientia Pharmaceutica antidepressant anxiolytic sedative hypnotic anticonvulsant <i>Malpighia mexicana</i> |
| title | Neuropharmacological Activity of the Acetonic Extract of <i>Malpighia mexicana</i> A. Juss. and Its Phytochemical Profile |
| title_full | Neuropharmacological Activity of the Acetonic Extract of <i>Malpighia mexicana</i> A. Juss. and Its Phytochemical Profile |
| title_fullStr | Neuropharmacological Activity of the Acetonic Extract of <i>Malpighia mexicana</i> A. Juss. and Its Phytochemical Profile |
| title_full_unstemmed | Neuropharmacological Activity of the Acetonic Extract of <i>Malpighia mexicana</i> A. Juss. and Its Phytochemical Profile |
| title_short | Neuropharmacological Activity of the Acetonic Extract of <i>Malpighia mexicana</i> A. Juss. and Its Phytochemical Profile |
| title_sort | neuropharmacological activity of the acetonic extract of i malpighia mexicana i a juss and its phytochemical profile |
| topic | antidepressant anxiolytic sedative hypnotic anticonvulsant <i>Malpighia mexicana</i> |
| url | https://www.mdpi.com/2218-0532/91/4/47 |
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