Gut Microbiota Signatures in Tumor, Para-Cancerous, Normal Mucosa, and Feces in Colorectal Cancer Patients

Background: Association studies have linked microbiome alterations with colorectal cancer (CRC). However, differences in tumor, para-cancerous, normal mucosal, and fecal microbiota remain to be strengthened.Methods: We performed a study on the ecologically rich and taxonomically diverse of gut micro...

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Main Authors: Yanmin Li, Hong Cao, Bojian Fei, Qizhong Gao, Wanya Yi, Weifeng Han, Chuanqing Bao, Jianmin Xu, Wei Zhao, Feng Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-06-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2022.916961/full
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author Yanmin Li
Hong Cao
Bojian Fei
Qizhong Gao
Wanya Yi
Weifeng Han
Chuanqing Bao
Jianmin Xu
Wei Zhao
Feng Zhang
Feng Zhang
author_facet Yanmin Li
Hong Cao
Bojian Fei
Qizhong Gao
Wanya Yi
Weifeng Han
Chuanqing Bao
Jianmin Xu
Wei Zhao
Feng Zhang
Feng Zhang
author_sort Yanmin Li
collection DOAJ
description Background: Association studies have linked microbiome alterations with colorectal cancer (CRC). However, differences in tumor, para-cancerous, normal mucosal, and fecal microbiota remain to be strengthened.Methods: We performed a study on the ecologically rich and taxonomically diverse of gut microbiota using three types of colorectal mucosa (tumor mucosa, para-cancerous mucosa, normal mucosa) and feces from 98 CRC patients. Additionally, we profiled the microbiota in the fecal occult blood test (FOBT) positive and negative groups at different sampling sites.Results: We found striking variations between tumor mucosal microbiota and normal mucosal microbiota. However, there was no significant difference between tumor and para-cancerous mucosal microbiota, as well as between para-cancerous and normal mucosal microbiota, revealing that the para-cancerous mucosal microbiota was a transitional state between the tumor and normal mucosal microbiota. And the substantial shifts in the fecal microbiota compared to mucosal microbiota indicated the risk of using fecal microbiota to define mucosal microbiota. A strong correlation between FOBT positive and Fusobacterium was discovered, indicating this adherent-invasive genus was closely related to intestinal bleeding. Furthermore, we identified six key genera, including Fusobacterium, Gemella, Campylobacter, Peptostreptococcus, Alloprevotella, and Parvimonas, which appear to be consistently over-represented in tumor mucosa compared to normal mucosa and/or in mucosa compared to feces.Conclusion: Compositional alterations in the microbiota existed in three types of colorectal mucosa and feces in CRC patients. Six key genera may contribute to the topographic variances in the microbiota of tumor-bearing colorectum.
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spelling doaj.art-72d6704ef79d4b1cb213f2377cfbb8e52022-12-22T00:55:26ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-06-011010.3389/fcell.2022.916961916961Gut Microbiota Signatures in Tumor, Para-Cancerous, Normal Mucosa, and Feces in Colorectal Cancer PatientsYanmin Li0Hong Cao1Bojian Fei2Qizhong Gao3Wanya Yi4Weifeng Han5Chuanqing Bao6Jianmin Xu7Wei Zhao8Feng Zhang9Feng Zhang10State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaDepartment of Nutrition, Affiliated Hospital of Jiangnan University, Wuxi, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Jiangnan University, Wuxi, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Jiangnan University, Wuxi, ChinaDepartment of Nutrition, Affiliated Hospital of Jiangnan University, Wuxi, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Jiangnan University, Wuxi, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Jiangnan University, Wuxi, ChinaDepartment of Gastrointestinal Surgery, Affiliated Hospital of Jiangnan University, Wuxi, ChinaState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, ChinaDepartment of Nutrition, Affiliated Hospital of Jiangnan University, Wuxi, ChinaChinese Society of Nutritional Oncology, Beijing, ChinaBackground: Association studies have linked microbiome alterations with colorectal cancer (CRC). However, differences in tumor, para-cancerous, normal mucosal, and fecal microbiota remain to be strengthened.Methods: We performed a study on the ecologically rich and taxonomically diverse of gut microbiota using three types of colorectal mucosa (tumor mucosa, para-cancerous mucosa, normal mucosa) and feces from 98 CRC patients. Additionally, we profiled the microbiota in the fecal occult blood test (FOBT) positive and negative groups at different sampling sites.Results: We found striking variations between tumor mucosal microbiota and normal mucosal microbiota. However, there was no significant difference between tumor and para-cancerous mucosal microbiota, as well as between para-cancerous and normal mucosal microbiota, revealing that the para-cancerous mucosal microbiota was a transitional state between the tumor and normal mucosal microbiota. And the substantial shifts in the fecal microbiota compared to mucosal microbiota indicated the risk of using fecal microbiota to define mucosal microbiota. A strong correlation between FOBT positive and Fusobacterium was discovered, indicating this adherent-invasive genus was closely related to intestinal bleeding. Furthermore, we identified six key genera, including Fusobacterium, Gemella, Campylobacter, Peptostreptococcus, Alloprevotella, and Parvimonas, which appear to be consistently over-represented in tumor mucosa compared to normal mucosa and/or in mucosa compared to feces.Conclusion: Compositional alterations in the microbiota existed in three types of colorectal mucosa and feces in CRC patients. Six key genera may contribute to the topographic variances in the microbiota of tumor-bearing colorectum.https://www.frontiersin.org/articles/10.3389/fcell.2022.916961/fullcolorectal cancerFusobacterium16S rRNAgut microbiotafecal occult blood test
spellingShingle Yanmin Li
Hong Cao
Bojian Fei
Qizhong Gao
Wanya Yi
Weifeng Han
Chuanqing Bao
Jianmin Xu
Wei Zhao
Feng Zhang
Feng Zhang
Gut Microbiota Signatures in Tumor, Para-Cancerous, Normal Mucosa, and Feces in Colorectal Cancer Patients
Frontiers in Cell and Developmental Biology
colorectal cancer
Fusobacterium
16S rRNA
gut microbiota
fecal occult blood test
title Gut Microbiota Signatures in Tumor, Para-Cancerous, Normal Mucosa, and Feces in Colorectal Cancer Patients
title_full Gut Microbiota Signatures in Tumor, Para-Cancerous, Normal Mucosa, and Feces in Colorectal Cancer Patients
title_fullStr Gut Microbiota Signatures in Tumor, Para-Cancerous, Normal Mucosa, and Feces in Colorectal Cancer Patients
title_full_unstemmed Gut Microbiota Signatures in Tumor, Para-Cancerous, Normal Mucosa, and Feces in Colorectal Cancer Patients
title_short Gut Microbiota Signatures in Tumor, Para-Cancerous, Normal Mucosa, and Feces in Colorectal Cancer Patients
title_sort gut microbiota signatures in tumor para cancerous normal mucosa and feces in colorectal cancer patients
topic colorectal cancer
Fusobacterium
16S rRNA
gut microbiota
fecal occult blood test
url https://www.frontiersin.org/articles/10.3389/fcell.2022.916961/full
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