Bioactive Regeneration Potential of the Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-<span style="font-variant: small-caps">l</span>-Lactide-Co-Glycolide Biomaterial in Maxillofacial Reconstructive Surgery: An In Vivo Preliminary Study
Uncalcined/unsintered hydroxyapatite (HA) and poly-<span style="font-variant: small-caps;">l</span>-lactide-co-glycolide (u-HA/PLLA/PGA) are novel bioresorbable bioactive materials with bone regeneration characteristics and have been used to treat mandibular defects in a rat mo...
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MDPI AG
2021-05-01
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| author | Shinji Ishizuka Quang Ngoc Dong Huy Xuan Ngo Yunpeng Bai Jingjing Sha Erina Toda Tatsuo Okui Takahiro Kanno |
| author_facet | Shinji Ishizuka Quang Ngoc Dong Huy Xuan Ngo Yunpeng Bai Jingjing Sha Erina Toda Tatsuo Okui Takahiro Kanno |
| author_sort | Shinji Ishizuka |
| collection | DOAJ |
| description | Uncalcined/unsintered hydroxyapatite (HA) and poly-<span style="font-variant: small-caps;">l</span>-lactide-co-glycolide (u-HA/PLLA/PGA) are novel bioresorbable bioactive materials with bone regeneration characteristics and have been used to treat mandibular defects in a rat model. However, the bone regenerative interaction with the periosteum, the inflammatory response, and the degradation of this material have not been examined. In this study, we used a rat mandible model to compare the above features in u-HA/PLLA/PGA and uncalcined/unsintered HA and poly-<span style="font-variant: small-caps;">l</span>-lactic acid (u-HA/PLLA). We divided 11 male Sprague–Dawley rats into 3- and 16-week groups. In each group, we assessed the characteristics of a u-HA/PLLA/PGA sheet covering the right mandibular angle and a u-HA/PLLA sheet covering the left mandibular angle in three rats each, and one rat was used as a sham control. The remaining three rats in the 16-week group were used for a degradation assessment and received both sheets of material as in the material assessment subgroup. At 3 and 16 weeks after surgery, the rats were sacrificed, and mandible specimens were subjected to micro-computed tomography, histological analysis, and immunohistochemical staining. The results indicated that the interaction between the periosteum and u-HA/PLLA/PGA material produced significantly more new bone regeneration with a lower inflammatory response and a faster resorption rate compared to u-HA/PLLA alone. These findings may indicate that this new biomaterial has ideal potential in treating maxillofacial defects of the midface and orbital regions. |
| first_indexed | 2024-03-10T11:34:41Z |
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| id | doaj.art-72d7e241dbe04dfb811af3af3cf4400f |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T11:34:41Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-72d7e241dbe04dfb811af3af3cf4400f2023-11-21T18:57:22ZengMDPI AGMaterials1996-19442021-05-01149246110.3390/ma14092461Bioactive Regeneration Potential of the Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-<span style="font-variant: small-caps">l</span>-Lactide-Co-Glycolide Biomaterial in Maxillofacial Reconstructive Surgery: An In Vivo Preliminary StudyShinji Ishizuka0Quang Ngoc Dong1Huy Xuan Ngo2Yunpeng Bai3Jingjing Sha4Erina Toda5Tatsuo Okui6Takahiro Kanno7Department of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanUncalcined/unsintered hydroxyapatite (HA) and poly-<span style="font-variant: small-caps;">l</span>-lactide-co-glycolide (u-HA/PLLA/PGA) are novel bioresorbable bioactive materials with bone regeneration characteristics and have been used to treat mandibular defects in a rat model. However, the bone regenerative interaction with the periosteum, the inflammatory response, and the degradation of this material have not been examined. In this study, we used a rat mandible model to compare the above features in u-HA/PLLA/PGA and uncalcined/unsintered HA and poly-<span style="font-variant: small-caps;">l</span>-lactic acid (u-HA/PLLA). We divided 11 male Sprague–Dawley rats into 3- and 16-week groups. In each group, we assessed the characteristics of a u-HA/PLLA/PGA sheet covering the right mandibular angle and a u-HA/PLLA sheet covering the left mandibular angle in three rats each, and one rat was used as a sham control. The remaining three rats in the 16-week group were used for a degradation assessment and received both sheets of material as in the material assessment subgroup. At 3 and 16 weeks after surgery, the rats were sacrificed, and mandible specimens were subjected to micro-computed tomography, histological analysis, and immunohistochemical staining. The results indicated that the interaction between the periosteum and u-HA/PLLA/PGA material produced significantly more new bone regeneration with a lower inflammatory response and a faster resorption rate compared to u-HA/PLLA alone. These findings may indicate that this new biomaterial has ideal potential in treating maxillofacial defects of the midface and orbital regions.https://www.mdpi.com/1996-1944/14/9/2461bone regenerationRunx2osteocalcinCD68periostinosteoconductivity |
| spellingShingle | Shinji Ishizuka Quang Ngoc Dong Huy Xuan Ngo Yunpeng Bai Jingjing Sha Erina Toda Tatsuo Okui Takahiro Kanno Bioactive Regeneration Potential of the Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-<span style="font-variant: small-caps">l</span>-Lactide-Co-Glycolide Biomaterial in Maxillofacial Reconstructive Surgery: An In Vivo Preliminary Study Materials bone regeneration Runx2 osteocalcin CD68 periostin osteoconductivity |
| title | Bioactive Regeneration Potential of the Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-<span style="font-variant: small-caps">l</span>-Lactide-Co-Glycolide Biomaterial in Maxillofacial Reconstructive Surgery: An In Vivo Preliminary Study |
| title_full | Bioactive Regeneration Potential of the Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-<span style="font-variant: small-caps">l</span>-Lactide-Co-Glycolide Biomaterial in Maxillofacial Reconstructive Surgery: An In Vivo Preliminary Study |
| title_fullStr | Bioactive Regeneration Potential of the Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-<span style="font-variant: small-caps">l</span>-Lactide-Co-Glycolide Biomaterial in Maxillofacial Reconstructive Surgery: An In Vivo Preliminary Study |
| title_full_unstemmed | Bioactive Regeneration Potential of the Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-<span style="font-variant: small-caps">l</span>-Lactide-Co-Glycolide Biomaterial in Maxillofacial Reconstructive Surgery: An In Vivo Preliminary Study |
| title_short | Bioactive Regeneration Potential of the Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-<span style="font-variant: small-caps">l</span>-Lactide-Co-Glycolide Biomaterial in Maxillofacial Reconstructive Surgery: An In Vivo Preliminary Study |
| title_sort | bioactive regeneration potential of the newly developed uncalcined unsintered hydroxyapatite and poly span style font variant small caps l span lactide co glycolide biomaterial in maxillofacial reconstructive surgery an in vivo preliminary study |
| topic | bone regeneration Runx2 osteocalcin CD68 periostin osteoconductivity |
| url | https://www.mdpi.com/1996-1944/14/9/2461 |
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